PHETA1 (NM_144671) Human Tagged ORF Clone
CAT#: RG206625
- TrueORF®
FAM109A (tGFP-tagged) - Human family with sequence similarity 109, member A (FAM109A), transcript variant 2
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_144671" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | PHETA1 |
Synonyms | FAM109A; IPIP27A; SES1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG206625 representing NM_144671
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCTGAACGAGCGCAGCCTGGCCTTCTACGCCACCTGTGACGCCCCGGTGGACAATGCAGGCTTCC TGTACAAGAAGGGTGGGCGGCACGCGGCCTACCACCGGCGCTGGTTCGTGCTGCGCGGGAACATGCTCTT CTACTTCGAGGACGCTGCCAGCCGTGAGCCCGTGGGCGTCATCATCCTGGAGGGCTGCACTGTGGAGCTG GTGGAGGCCGCCGAGGAGTTCGCCTTCGCTGTGCGCTTTGCGGGGACCCGGGCGCGCACCTACGTGCTGG CCGCTGAGAGTCAGGATGCCATGGAGGGCTGGGTCAAGGCCCTGTCGCGTGCCAGCTTCGACTACCTGCG GCTGGTGGTGCGCGAGCTGGAGCAGCAGCTGGCGGCTGTACGTGGCGGGGGTGGCATGGCCCTGCCCCAG CCCCAGCCCCAGTCCCTGCCCTTGCCCCCGTCCCTGCCCTCTGCCCTGGCCCCAGTCCCATCCCTGCCTT CTGCCCCAGCCCCGGTCCCAGCCCTGCCCCTGCCCCGCCGGCCCAGTGCCCTCCCGCCCAAGGAGAATGG CTGCGCTGTCTGGAGCACTGAGGCCACCTTCAGGCCTGGACCCGAGCCCCCTCCACCACCGCCTCGCCGC CGGGCCTCGGCACCCCACGGGCCCCTGGACATGGCCCCCTTCGCCCGGCTGCACGAGTGCTATGGCCAGG AGATCCGGGCCCTGCGTGGCCAGTGGCTCAGCAGCCGGGTCCAGCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG206625 representing NM_144671
Red=Cloning site Green=Tags(s) MKLNERSLAFYATCDAPVDNAGFLYKKGGRHAAYHRRWFVLRGNMLFYFEDAASREPVGVIILEGCTVEL VEAAEEFAFAVRFAGTRARTYVLAAESQDAMEGWVKALSRASFDYLRLVVRELEQQLAAVRGGGGMALPQ PQPQSLPLPPSLPSALAPVPSLPSAPAPVPALPLPRRPSALPPKENGCAVWSTEATFRPGPEPPPPPPRR RASAPHGPLDMAPFARLHECYGQEIRALRGQWLSSRVQP TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_144671 |
ORF Size | 747 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_144671.5 |
RefSeq Size | 3054 bp |
RefSeq ORF | 750 bp |
Locus ID | 144717 |
UniProt ID | Q8N4B1 |
Cytogenetics | 12q24.12 |
Gene Summary | This gene encodes a protein that localizes to the endosome and interacts with the enzyme, inositol polyphosphate 5-phosphatase OCRL-1. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206625 | FAM109A (Myc-DDK-tagged)-Human family with sequence similarity 109, member A (FAM109A), transcript variant 2 |
USD 300.00 |
|
RC206625L3 | Lenti ORF clone of Human family with sequence similarity 109, member A (FAM109A), transcript variant 2, Myc-DDK-tagged |
USD 600.00 |
|
RC206625L4 | Lenti ORF clone of Human family with sequence similarity 109, member A (FAM109A), transcript variant 2, mGFP tagged |
USD 600.00 |
|
SC123183 | FAM109A (untagged)-Human family with sequence similarity 109, member A (FAM109A), transcript variant 2 |
USD 300.00 |
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