TEKT3 (NM_031898) Human Tagged ORF Clone

CAT#: RG206395

TEKT3 (tGFP-tagged) - Human tektin 3 (TEKT3)

Product Images

Circular map for RG206395

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TEKT3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206395 representing NM_031898
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACGTGTAGGTTGTACTTTAACGACAACTTACGCCCACCCTAGACCAACACCAACCAACTTTCTAC
  CAGCCATCAGTACCATGGCCTCAAGCTACAGGGACCGCTTTCCCCACTCCAATTTGACCCATAGCCTGAG
  CCTTCCTTGGAGACCCAGCACATACTACAAAGTCGCCTCCAATTCCCCAAGCGTGGCCCCGTACTGCACC
  AGATCACAGAGGGTGTCCGAGAATACCATGCTTCCCTTTGTTTCCAACAGAACCACTTTCTTCACAAGAT
  ACACACCGGATGACTGGTACAGGTCCAATTTAACCAACTATCAAGAGTCCAACACTTCCCGACATAATTC
  GGAGAAACTAAGAGTGGATACATCTCGCCTGATTCAAGACAAATATCAACAAACAAGAAAAACTCAGGCA
  GACACAACCCAAAATCTGGGAGAACGTGTCAATGACATAGGGTTTTGGAAATCTGAAATCATTCATGAGT
  TGGATGAAATGATTGGAGAGACAAATGCACTTACTGATGTGAAGAAAAGACTGGAGCGGGCTTTGATGGA
  GACTGAAGCCCCTCTTCAGGTAGCCCGAGAATGTCTATTTCATCGAGAAAAGAGAATGGGAATCGACCTA
  GTTCACGATGAAGTTGAAGCACAACTGCTGACGGAAGTTGATACTATTCTGTGTTGTCAAGAAAGAATGA
  AGCTACATTTGGATAAGGCTATTGCCCAACTTGCAGCCAACAGAGCGTCCCAGCATGAGCTGGAAAAGGA
  CCTGAGTGACAAACAGACGGCTTACCGGATCAACGACAAATGCCACCACCTGCGCAACACATCAGACGGT
  GTCGGCTACTTCCGCGGAGTGGAGAGGGTCGATGCAACTGTCTCAGTGCCTGAGTCCTGGGCCAAATTTA
  CAGATGACAATATTCTCCGCTCCCAGAGTGAACGGGCAGCTTCCGCTAAGCTAAGAGACGACATTGAAAA
  CCTCTTGGTTGTGACTGCCAATGAGATGTGGAATCAGTTCAACAAAGTGAACTTGTCTTTCACCAATCGC
  ATTGCTGAGACTGCAGATGCTAAGAATAAGATTCAGACGCACTTAGCAAAGACCCTGCAGGAGATTTTCC
  AGACTGAAATGACCATAGAATCCATCAAGAAGGCCATCAAGGACAAGACTGCCTTCCTGAAGGTGGCTCA
  GACCAGACTGGATGAGCGCACAAGACGGCCGAACATTGAGTTGTGCCGAGACATGGCTCAGCTACGCCTT
  GTTAACGAGGTACACGAGGTTGACGACACCATCCAGACCCTGCAGCAGCGCCTGAGGGATGCAGAGGACA
  CCCTGCAGTCGCTGGTCCACATCAAAGCCACCCTCGAGTATGACCTGGCTGTCAAAGCCAATTCCCTGTA
  CATCGACCAGGAAAAATGCATGAGCATGCGCAAGAGCTACCCCAACACCCTCCGGCTGGTCGGCTTCTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206395 representing NM_031898
  Red=Cloning site Green=Tags(s)
  
  MERVGCTLTTTYAHPRPTPTNFLPAISTMASSYRDRFPHSNLTHSLSLPWRPSTYYKVASNSPSVAPYCT
  RSQRVSENTMLPFVSNRTTFFTRYTPDDWYRSNLTNYQESNTSRHNSEKLRVDTSRLIQDKYQQTRKTQA
  DTTQNLGERVNDIGFWKSEIIHELDEMIGETNALTDVKKRLERALMETEAPLQVARECLFHREKRMGIDL
  VHDEVEAQLLTEVDTILCCQERMKLHLDKAIAQLAANRASQHELEKDLSDKQTAYRINDKCHHLRNTSDG
  VGYFRGVERVDATVSVPESWAKFTDDNILRSQSERAASAKLRDDIENLLVVTANEMWNQFNKVNLSFTNR
  IAETADAKNKIQTHLAKTLQEIFQTEMTIESIKKAIKDKTAFLKVAQTRLDERTRRPNIELCRDMAQLRL
  VNEVHEVDDTIQTLQQRLRDAEDTLQSLVHIKATLEYDLAVKANSLYIDQEKCMSMRKSYPNTLRLVGFC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031898
• ORF Size: 1470 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031898.1, NP_114104.1
• RefSeq Size: 1707 bp
• RefSeq ORF: 1473 bp
• Locus ID: 64518
• UniProt ID: Q9BXF9
• Cytogenetics: 17p12
• Gene Summary: This gene product belongs to the tektin family of proteins. Tektins comprise a family of filament-forming proteins that are coassembled with tubulins to form ciliary and flagellar microtubules. The exact function of this gene is not known. [provided by RefSeq, Jul 2008]