SPG7 (NM_003119) Human Tagged ORF Clone

CAT#: RG206340

SPG7 (tGFP-tagged) - Human spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Images

Circular map for RG206340

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SPG7
• Synonyms: CAR; CMAR; PGN; SPG5C
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206340 representing NM_003119
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTGCTGCTGCTGCTGCTCCGTGCCCTCCGCCGGGGTCCAGGCCCGGGTCCTCGGCCGCTGTGGG
  GCCCAGGCCCGGCCTGGAGTCCAGGGTTCCCCGCCAGGCCCGGGAGGGGGCGGCCGTACATGGCCAGCAG
  GCCTCCGGGGGACCTCGCCGAGGCTGGAGGCCGAGCTCTGCAGAGCTTACAATTGAGACTGCTAACCCCT
  ACCTTTGAAGGGATCAACGGATTGTTGTTGAAACAACATTTAGTTCAGAATCCAGTCAGACTCTGGCAAC
  TTTTAGGTGGTACTTTCTATTTTAACACCTCAAGGTTGAAGCAGAAGAATAAGGAGAAGGATAAGTCGAA
  GGGGAAGGCGCCTGAAGAGGACGAAGAGGAGAGGAGACGCCGTGAGCGGGACGACCAGATGTACCGAGAG
  CGGCTGCGCACCTTGCTGGTCATCGCGGTTGTCATGAGCCTCCTGAATGCTCTCAGCACCAGCGGAGGCA
  GCATTTCCTGGAACGACTTTGTCCACGAGATGCTGGCCAAGGGCGAGGTGCAGCGCGTCCAGGTGGTGCC
  TGAGAGCGACGTGGTGGAAGTCTACCTGCACCCTGGAGCCGTGGTGTTTGGGCGGCCTCGGCTAGCCTTG
  ATGTACCGAATGCAGGTTGCAAATATTGACAAGTTTGAAGAGAAGCTTCGAGCAGCTGAAGATGAGCTGA
  ATATCGAGGCCAAGGACAGGATCCCAGTTTCCTACAAGCGAACAGGATTCTTTGGAAATGCCCTGTACTC
  TGTGGGGATGACGGCAGTGGGCCTGGCCATCCTGTGGTATGTTTTCCGTCTGGCCGGGATGACTGGAAGG
  GAAGGTGGATTCAGTGCTTTTAATCAGCTTAAAATGGCTCGTTTCACCATTGTGGATGGGAAGATGGGGA
  AAGGAGTCAGCTTCAAAGACGTGGCAGGAATGCACGAAGCCAAACTGGAAGTCCGCGAGTTTGTGGATTA
  TCTGAAGAGCCCAGAACGCTTCCTCCAGCTTGGCGCCAAGGTCCCAAAGGGCGCACTGCTGCTCGGCCCC
  CCCGGCTGTGGGAAGACGCTGCTGGCCAAGGCGGTGGCCACGGAGGCTCAGGTGCCCTTCCTGGCGATGG
  CCGGCGCAGAGTTCGTGGAGGTCATTGGAGGCCTCGGCGCTGCCCGTGTGCGGAGCCTCTTTAAGGAAGC
  CCGAGCCCGGGCCCCCTGCATCGTCTACATCGATGAGATCGACGCGGTGGGCAAGAAGCGCTCCACCACC
  ATGTCCGGCTTCTCCAACACGGAGGAGGAGCAGACGCTCAACCAGCTTCTGGTAGAAATGGATGGAATGG
  GTACCACAGACCATGTCATCGTCCTGGCGTCCACGAACCGAGCTGACATTTTGGACGGTGCTCTGATGAG
  GCCAGGCCGACTGGACCGGCACGTCTTCATTGATCTCCCCACGCTGCAGGAGAGGCGGGAGATTTTTGAG
  CAGCACCTGAAGAGCCTGAAGCTGACCCAGTCCAGCACCTTTTACTCCCAGCGTCTGGCAGAGCTGACAC
  CAGGATTCAGTGGGGCTGACATCGCCAACATCTGCAATGAGGCTGCGCTGCACGCGGCGCGGGAGGGACA
  CACTTCCGTGCACACTCTCAACTTCGAGTACGCCGTGGAGCGCGTCCTCGCAGGGACTGCCAAAAAGAGT
  AAGATCCTGTCCAAGGAAGAACAGAAAGTGGTTGCGTTTCATGAGTCGGGCCACGCCTTGGTGGGCTGGA
  TGCTGGAGCACACGGAGGCCGTGATGAAGGTCTCCATAACCCCTCGGACAAACGCCGCCCTGGGCTTTGC
  TCAGATGCTCCCCAGAGACCAGCACCTCTTCACCAAGGAGCAGCTGTTTGAGCGGATGTGCATGGCCCTG
  GGAGGACGGGCCTCGGAAGCACTGTCCTTCAACGAGGTCACTTCTGGGGCACAGGACGACCTGAGGAAGG
  TCACCCGCATCGCCTACTCCATGGTGAAGCAGTTTGGGATGGCACCTGGCATCGGGCCCATCTCCTTCCC
  TGAGGCGCAGGAGGGCCTCATGGGCATCGGGCGGCGCCCCTTCAGCCAAGGCCTGCAGCAGATGATGGAC
  CATGAAGCAAGACTGCTGGTGGCCAAGGCCTACAGACACACCGAGAAGGTGCTGCAGGACAACCTGGACA
  AGTTGCAGGCGCTGGCAAACGCCCTTCTGGAAAAGGAAGTGATAAACTATGAGGACATTGAGGCTCTCAT
  TGGCCCGCCGCCCCATGGGCCGAAGAAAATGATCGCACCGCAGAGGTGGATCGACGCCCAGAGGGAGAAA
  CAGGACTTGGGCGAGGAGGAGACCGAAGAGACCCAGCAGCCTCCACTTGGAGGCGAAGAGCCGACTTGGC
  CCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206340 representing NM_003119
  Red=Cloning site Green=Tags(s)
  
  MAVLLLLLRALRRGPGPGPRPLWGPGPAWSPGFPARPGRGRPYMASRPPGDLAEAGGRALQSLQLRLLTP
  TFEGINGLLLKQHLVQNPVRLWQLLGGTFYFNTSRLKQKNKEKDKSKGKAPEEDEEERRRRERDDQMYRE
  RLRTLLVIAVVMSLLNALSTSGGSISWNDFVHEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLAL
  MYRMQVANIDKFEEKLRAAEDELNIEAKDRIPVSYKRTGFFGNALYSVGMTAVGLAILWYVFRLAGMTGR
  EGGFSAFNQLKMARFTIVDGKMGKGVSFKDVAGMHEAKLEVREFVDYLKSPERFLQLGAKVPKGALLLGP
  PGCGKTLLAKAVATEAQVPFLAMAGAEFVEVIGGLGAARVRSLFKEARARAPCIVYIDEIDAVGKKRSTT
  MSGFSNTEEEQTLNQLLVEMDGMGTTDHVIVLASTNRADILDGALMRPGRLDRHVFIDLPTLQERREIFE
  QHLKSLKLTQSSTFYSQRLAELTPGFSGADIANICNEAALHAAREGHTSVHTLNFEYAVERVLAGTAKKS
  KILSKEEQKVVAFHESGHALVGWMLEHTEAVMKVSITPRTNAALGFAQMLPRDQHLFTKEQLFERMCMAL
  GGRASEALSFNEVTSGAQDDLRKVTRIAYSMVKQFGMAPGIGPISFPEAQEGLMGIGRRPFSQGLQQMMD
  HEARLLVAKAYRHTEKVLQDNLDKLQALANALLEKEVINYEDIEALIGPPPHGPKKMIAPQRWIDAQREK
  QDLGEEETEETQQPPLGGEEPTWPK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003119
• ORF Size: 2385 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003119.2, NP_003110.1
• RefSeq Size: 3096 bp
• RefSeq ORF: 2388 bp
• Locus ID: 6687
• UniProt ID: Q9UQ90
• Cytogenetics: 16q24.3
• Domains: Peptidase_M41, AAA, AAA
• Protein Families: Protease, Transmembrane
• Gene Summary: This gene encodes a mitochondrial metalloprotease protein that is a member of the AAA family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Mutations in this gene cause autosomal recessive spastic paraplegia 7. Two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2014]