TPTE (NM_199259) Human Tagged ORF Clone

CAT#: RG206018

TPTE (tGFP-tagged) - Human transmembrane phosphatase with tensin homology (TPTE), transcript variant 2

Product Images

Circular map for RG206018

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TPTE
• Synonyms: CT44; PTEN2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206018 representing NM_199259
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGAAAGTCCTGATCCGACTGACCTGGCGGGAGTCATCATTGAGCTCGGCCCCAATGACAGTCCAC
  AGACAAGTGAATTTAAAGGAGCAACCGAGGAGGCACCTGCGAAAGAAAGTGTGTTAGCACGACTTTCCAA
  GTTTGAAGTTGAAGATGCTGAAAATGTTGCTTCATATGACAGCAAGATTAAGAAAATTGTGCATTCAATT
  GTATCATCCTTTGCATTTGGACTATTTGGAGTTTTCCTGGTCTTACTGGATGTCACTCTCATCCTTGCCG
  ACCTAATTTTCACTGACAGCAAACTTTATATTCCTTTGGAGTATCGTTCTATTTCTCTAGCTATTGCCTT
  ATTTTTTCTCATGGATGTTCTTCTTCGAGTATTTGTAGAAAGGAGACAGCAGTATTTTTCTGACTTATTT
  AACATTTTAGATACTGCCATTATTGTGATTCTTCTGCTGGTTGATGTCGTTTACATTTTTTTTGACATTA
  AGTTGCTTAGGAATATTCCCAGATGGACACATTTACTTCGACTTCTACGACTTATTATTCTGTTAAGAAT
  TTTTCATCTGTTTCATCAAAAAAGACAACTTGAAAAGCTGATAAGAAGGCGGGTTTCAGAAAACAAAAGG
  CGATACACAAGGGATGGATTTGACCTAGACCTCACTTACGTTACAGAACGTATTATTGCTATGTCATTTC
  CATCTTCTGGAAGGCAGTCTTTCTATAGAAATCCAATCAAGGAAGTTGTGCGGTTTCTAGATAAGAAACA
  CCGAAACCACTATCGAGTCTACAATCTATGCAGTGAAAGAGCTTACGATCCTAAGCACTTCCATAATAGG
  GTCGTTAGAATCATGATTGATGATCATAATGTCCCCACTCTACATCAGATGGTGGTTTTCACCAAGGAAG
  TAAATGAGTGGATGGCTCAAGATCTTGAAAACATCGTAGCGATTCACTGTAAAGGAGGCACAGATAGAAC
  AGGAACTATGGTTTGTGCCTTCCTTATTGCCTCTGAAATATGTTCAACTGCAAAGGAAAGCCTGTATTAT
  TTTGGAGAAAGGCGAACAGATAAAACCCACAGCGAAAAATTTCAGGGAGTAGAAACTCCTTCTCAGAAGA
  GATATGTTGCATATTTTGCACAAGTGAAACATCTCTACAACTGGAATCTCCCTCCAAGACGGATACTCTT
  TATAAAACACTTCATTATTTATTCGATTCCTCGTTATGTACGTGATCTAAAAATCCAAATAGAAATGGAG
  AAAAAGGTTGTCTTTTCCACTATTTCATTAGGAAAATGTTCGGTACTTGATAACATTACAACAGACAAAA
  TATTAATTGATGTATTCGACGGTCCACCTCTGTATGATGATGTGAAAGTGCAGTTTTTCTATTCGAATCT
  TCCTACATACTATGACAATTGCTCATTTTACTTCTGGTTGCACACATCTTTTATTGAAAATAACAGGCTT
  TATCTACCAAAAAATGAATTGGATAATCTACATAAACAAAAAGCACGGAGAATTTATCCATCAGATTTTG
  CCGTGGAGATACTTTTTGGCGAGAAAATGACTTCCAGTGATGTTGTAGCTGGATCCGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206018 representing NM_199259
  Red=Cloning site Green=Tags(s)
  
  MNESPDPTDLAGVIIELGPNDSPQTSEFKGATEEAPAKESVLARLSKFEVEDAENVASYDSKIKKIVHSI
  VSSFAFGLFGVFLVLLDVTLILADLIFTDSKLYIPLEYRSISLAIALFFLMDVLLRVFVERRQQYFSDLF
  NILDTAIIVILLLVDVVYIFFDIKLLRNIPRWTHLLRLLRLIILLRIFHLFHQKRQLEKLIRRRVSENKR
  RYTRDGFDLDLTYVTERIIAMSFPSSGRQSFYRNPIKEVVRFLDKKHRNHYRVYNLCSERAYDPKHFHNR
  VVRIMIDDHNVPTLHQMVVFTKEVNEWMAQDLENIVAIHCKGGTDRTGTMVCAFLIASEICSTAKESLYY
  FGERRTDKTHSEKFQGVETPSQKRYVAYFAQVKHLYNWNLPPRRILFIKHFIIYSIPRYVRDLKIQIEME
  KKVVFSTISLGKCSVLDNITTDKILIDVFDGPPLYDDVKVQFFYSNLPTYYDNCSFYFWLHTSFIENNRL
  YLPKNELDNLHKQKARRIYPSDFAVEILFGEKMTSSDVVAGSD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199259
• ORF Size: 1599 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199259.2, NP_954868.1
• RefSeq Size: 2145 bp
• RefSeq ORF: 1602 bp
• Locus ID: 7179
• UniProt ID: P56180
• Cytogenetics: 21p11.2
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a PTEN-related tyrosine phosphatase which may play a role in the signal transduction pathways of the endocrine or spermatogenic function of the testis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]