ARRDC4 (NM_183376) Human Tagged ORF Clone

CAT#: RG205534

ARRDC4 (tGFP-tagged) - Human arrestin domain containing 4 (ARRDC4)

Product Images

Circular map for RG205534

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ARRDC4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205534 representing NM_183376
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGGGGAGGCTGGGTGCGCGGCGGCCGTGGGTGCCGAGGGCCGCGTGAAGAGCCTGGGTCTGGTGT
  TCGAGGACGAGCGCAAGGGCTGCTATTCCAGCGGCGAGACAGTGGCCGGGCACGTGCTGCTGGAGGCGTC
  CGAGCCGGTGGCCCTGCGCGCGCTGCGCCTGGAGGCCCAGGGGCGCGCCACCGCCGCCTGGGGCCCGAGC
  ACCTGCCCCCGCGCCTCGGCCAGCACCGCGGCCCTGGCTGTCTTCTCGGAGGTGGAGTACCTGAACGTGC
  GCCTCAGCCTGCGGGAGCCCCCGGCCGGTGAAGGCATCATTTTATTACAGCCTGGAAAACATGAATTTCC
  ATTTCGCTTTCAACTTCCATCTGAACCTTTGGTCACCTCGTTTACTGGGAAATATGGAAGCATTCAGTAC
  TGTGTGCGGGCAGTGTTGGAACGACCCAAGGTACCTGATCAGAGTGTAAAGCGGGAACTCCAGGTTGTTA
  GTCATGTCGATGTCAACACACCAGCATTATTAACCCCTGTATTGAAAACTCAAGAGAAAATGGTTGGCTG
  TTGGTTTTTCACTTCTGGTCCAGTCTCGCTGAGTGCCAAAATTGAAAGAAAGGGATACTGTAATGGAGAA
  GCTATTCCAATCTATGCAGAAATAGAAAATTGTTCCTCTCGTCTGATTGTTCCAAAGGCTGCTATTTTCC
  AAACGCAGACATATTTGGCTAGTGGAAAAACAAAGACCATTCGACACATGGTCGCCAATGTGCGAGGAAA
  CCACATCGCTTCTGGGAGCACAGACACATGGAATGGGAAAACGCTAAAAATCCCACCTGTTACTCCATCC
  ATCCTGGATTGCTGCATTATCAGAGTGGACTATTCCTTAGCTGTATACATTCACATTCCTGGTGCTAAAA
  AATTGATGCTCGAACTGCCATTAGTGATCGGTACAATTCCATATAATGGTTTTGGCAGCAGAAACTCCAG
  CATTGCCAGCCAGTTCAGTATGGATATGAGCTGGTTGACACTGACCCTGCCAGAGCAGCCTGAAGCACCA
  CCAAATTATGCAGATGTGGTATCAGAGGAAGAATTCTCTAGACACATTCCTCCTTACCCTCAACCCCCTA
  ACTGTGAGGGAGAAGTGTGCTGTCCTGTGTTTGCCTGTATACAAGAATTCCGGTTTCAACCCCCACCTCT
  TTATTCAGAGGTTGACCCACATCCTAGCGACGTAGAAGAGAGCCAGCCTGTTTCCTTCATTCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205534 representing NM_183376
  Red=Cloning site Green=Tags(s)
  
  MGGEAGCAAAVGAEGRVKSLGLVFEDERKGCYSSGETVAGHVLLEASEPVALRALRLEAQGRATAAWGPS
  TCPRASASTAALAVFSEVEYLNVRLSLREPPAGEGIILLQPGKHEFPFRFQLPSEPLVTSFTGKYGSIQY
  CVRAVLERPKVPDQSVKRELQVVSHVDVNTPALLTPVLKTQEKMVGCWFFTSGPVSLSAKIERKGYCNGE
  AIPIYAEIENCSSRLIVPKAAIFQTQTYLASGKTKTIRHMVANVRGNHIASGSTDTWNGKTLKIPPVTPS
  ILDCCIIRVDYSLAVYIHIPGAKKLMLELPLVIGTIPYNGFGSRNSSIASQFSMDMSWLTLTLPEQPEAP
  PNYADVVSEEEFSRHIPPYPQPPNCEGEVCCPVFACIQEFRFQPPPLYSEVDPHPSDVEESQPVSFIL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_183376
• ORF Size: 1254 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183376.3
• RefSeq Size: 4074 bp
• RefSeq ORF: 1257 bp
• Locus ID: 91947
• UniProt ID: Q8NCT1
• Cytogenetics: 15q26.2
• Gene Summary: Functions as an adapter recruiting ubiquitin-protein ligases to their specific substrates (By similarity). Plays a role in endocytosis of activated G protein-coupled receptors (GPCRs) (Probable). Through an ubiquitination-dependent mechanism plays also a role in the incorporation of SLC11A2 into extracellular vesicles (By similarity). May play a role in glucose uptake (PubMed:19605364).[UniProtKB/Swiss-Prot Function]