Neurotensin Receptor 2 (NTSR2) (NM_012344) Human Tagged ORF Clone

CAT#: RG205343

NTSR2 (tGFP-tagged) - Human neurotensin receptor 2 (NTSR2)

Product Images

Circular map for RG205343

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Neurotensin Receptor 2
• Synonyms: NTR2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205343 representing NM_012344
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACCAGCAGCCCGCGGCCCCCGCGGCCCAGCTCCAACCCGGGGCTGAGCCTGGACGCCCGGCTGG
  GCGTGGACACTCGCCTCTGGGCCAAGGTGCTGTTCACCGCGCTCTACGCACTCATCTGGGCGCTGGGCGC
  GGCGGGCAATGCGCTGTCCGTGCACGTGGTGCTGAAGGCGCGGGCCGGGCGAGCGGGGCGCCTGCGCCAC
  CACGTGCTCAGCCTGGCGCTCGCGGGCCTGCTGCTGCTGCTGGTCGGCGTGCCGGTGGAGCTCTACAGCT
  TCGTGTGGTTCCACTACCCCTGGGTCTTCGGCGACCTGGGCTGCCGCGGCTACTACTTCGTGCACGAGCT
  GTGCGCCTACGCCACGGTGCTGAGCGTGGCAGGCCTGAGCGCCGAGCGCTGCCTAGCCGTGTGCCAGCCC
  CTGCGTGCCCGCAGCCTGCTGACGCCACGCCGGACCCGGTGGCTGGTGGCGCTCTCGTGGGCCGCCTCGC
  TCGGCCTCGCCATGCCCATGGCCGTCATCATGGGGCAGAAGCACGAACTCGAGACGGCGGACGGGGAGCC
  GGAGCCCGCCTCGCGAGTGTGCACGGTGCTGGTGAGCCGCACCGCGCTCCAAGTCTTTATCCAGGTGAAT
  GTGCTGGTGTCCTTCGTGCTCCCCTTGGCACTAACTGCTTTCCTGAATGGGGTCACAGTGAGCCACCTGC
  TGGCCCTCTGCTCCCAAGTGCCGTCCACTTCTACCCCGGGCAGCTCCACCCCCAGCCGCCTGGAGCTGCT
  GAGTGAGGAGGGTCTCCTCAGCTTCATCGTATGGAAGAAGACCTTTATCCAGGGAGGCCAGGTCAGCCTG
  GTGAGACATAAAGACGTGCGCCGGATCCGCAGCCTCCAGCGCAGCGTCCAGGTTCTCAGAGCCATCGTGG
  TCATGTATGTCATCTGCTGGCTGCCGTACCATGCCCGCAGGCTCATGTACTGCTACGTACCTGATGACGC
  GTGGACTGACCCACTGTACAATTTCTACCACTACTTCTACATGGTGACCAACACACTTTTCTACGTCAGC
  TCAGCTGTGACTCCTCTTCTCTACAACGCCGTGTCCTCCTCCTTCAGAAGACTCTTCCTGGAAGCCGTCA
  GCTCCCTGTGTGGAGAGCACCACCCCATGAAGCGGTTACCCCCGAAGCCCCAGAGTCCCACCCTAATGGA
  TACAGCTTCAGGCTTTGGGGATCCCCCAGAAACCCGGACC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205343 representing NM_012344
  Red=Cloning site Green=Tags(s)
  
  METSSPRPPRPSSNPGLSLDARLGVDTRLWAKVLFTALYALIWALGAAGNALSVHVVLKARAGRAGRLRH
  HVLSLALAGLLLLLVGVPVELYSFVWFHYPWVFGDLGCRGYYFVHELCAYATVLSVAGLSAERCLAVCQP
  LRARSLLTPRRTRWLVALSWAASLGLAMPMAVIMGQKHELETADGEPEPASRVCTVLVSRTALQVFIQVN
  VLVSFVLPLALTAFLNGVTVSHLLALCSQVPSTSTPGSSTPSRLELLSEEGLLSFIVWKKTFIQGGQVSL
  VRHKDVRRIRSLQRSVQVLRAIVVMYVICWLPYHARRLMYCYVPDDAWTDPLYNFYHYFYMVTNTLFYVS
  SAVTPLLYNAVSSSFRRLFLEAVSSLCGEHHPMKRLPPKPQSPTLMDTASGFGDPPETRT
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012344
• ORF Size: 1230 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012344.2, NP_036476.1
• RefSeq Size: 1700 bp
• RefSeq ORF: 1233 bp
• Locus ID: 23620
• UniProt ID: O95665
• Cytogenetics: 2p25.1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: The protein encoded by this gene belongs to the G protein-coupled receptor family that activate a phosphatidylinositol-calcium second messenger system. Binding and pharmacological studies demonstrate that this receptor binds neurotensin as well as several other ligands already described for neurotensin NT1 receptor. However, unlike NT1 receptor, this gene recognizes, with high affinity, levocabastine, a histamine H1 receptor antagonist previously shown to compete with neurotensin for low-affinity binding sites in brain. These activities suggest that this receptor may be of physiological importance and that a natural agonist for the receptor may exist. [provided by RefSeq, Jul 2008]