THEG (NM_016585) Human Tagged ORF Clone

CAT#: RG205211

THEG (tGFP-tagged) - Human Theg homolog (mouse) (THEG), transcript variant 1

Product Images

Circular map for RG205211

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: THEG
• Synonyms: CT56; THEG1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205211 representing NM_016585
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGACAGCAGGCGAAGGTCACTCGGGAACCAGCCCAGCTCTGAGGCTGCGGGCAGGTCGGAAAGGG
  AGCAGGACGGCGACCCCCGTGGCCTCCAGAGCTCTGTGTACGAGAGCCGGCGGGTCACAGACCCCGAACG
  CCAGGACCTGGACAATGCAGAGCTGGGACCAGAAGACCCAGAAGAGGAGCTTCCCCCCGAGGAGGTGGCC
  GGGGAGGAGTTCCCGGAGACCCTGGATCCCAAAGAGGCACTTTCTGAGTTGGAGAGAGTCCTGGACAAGG
  ACTTGGAAGAGGACATTCCTGAAATCAGCCGGCTGTCCATCAGCCAGAAGCTCCCCAGCACCACCATGAC
  CAAAGCAAGGAAGAGGAGGAGGCGGAGGAGGCTCATGGAGCTGGCAGAGCCCAAGATAAACTGGCAAGTC
  CTGAAAGACAGGAAGGGACGCTGTGGTAAGGGGTATGCCTGGATCTCCCCATGTAAGATGAGCTTGCACT
  TCTGTCTCTGCTGGCCCTCTGTGTACTGGACCGAGCGGTTCCTTGAGGACACCACCCTCACCATCACAGT
  GCCCGCGGTGTCCCGCCGCGTGGAGGAACTGTCTCGGCCCAAGAGATTCTACCTGGAATATTACAACAAC
  AACAGGACGACTCCTGTCTGGCCCATTCCTCGGTCCTCCCTGGAATACAGAGCGTCGAGTCGCCTGAAGG
  AACTGGCCGCCCCGAAGATTCGTGATAACTTCTGGAGCATGCCCATGTCTGAGGTGTCCCAGGTATCCAG
  GGCAGCCCAAATGGCAGTCCCCAGCTCGCGGATCCTCCAGTTGTCAAAGCCGAAGGCCCCAGCCACCCTC
  TTGGAAGAGTGGGACCCCGTGCCAAAACCCAAGCCACATGTGTCAGACCATAACCGCCTCCTTCACTTGG
  CCAGGCCCAAAGCTCAGTCGGACAAGTGCGTTCCTGACCGAGATCCTCGCTGGGAGGTGCTGGATGTCAC
  CAAGAAGGTGGTGGCCAGCCCCCGGATCATCTCCCTGGCCAAGCCCAAAGTGCGCAAGGGCCTCAACGAG
  GGATACGACAGGCGTCCCCTCGCCTCTATGAGCTTGCCACCCCCAAAAGCATCACCAGAAAAGTGTGATC
  AACCCAGGCCTGGCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205211 representing NM_016585
  Red=Cloning site Green=Tags(s)
  
  MGDSRRRSLGNQPSSEAAGRSEREQDGDPRGLQSSVYESRRVTDPERQDLDNAELGPEDPEEELPPEEVA
  GEEFPETLDPKEALSELERVLDKDLEEDIPEISRLSISQKLPSTTMTKARKRRRRRRLMELAEPKINWQV
  LKDRKGRCGKGYAWISPCKMSLHFCLCWPSVYWTERFLEDTTLTITVPAVSRRVEELSRPKRFYLEYYNN
  NRTTPVWPIPRSSLEYRASSRLKELAAPKIRDNFWSMPMSEVSQVSRAAQMAVPSSRILQLSKPKAPATL
  LEEWDPVPKPKPHVSDHNRLLHLARPKAQSDKCVPDRDPRWEVLDVTKKVVASPRIISLAKPKVRKGLNE
  GYDRRPLASMSLPPPKASPEKCDQPRPGL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016585
• ORF Size: 1137 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016585.5
• RefSeq Size: 1337 bp
• RefSeq ORF: 1140 bp
• Locus ID: 51298
• UniProt ID: Q9P2T0
• Cytogenetics: 19p13.3
• Gene Summary: This gene is specifically expressed in the nucleus of haploid male germ cells. The orthologous gene in mice encodes a protein that may play a role in protein assembly through interactions with T-complex protein 1 subunit epsilon. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]