GNB5 (NM_006578) Human Tagged ORF Clone
CAT#: RG204134
- TrueORF®
GNB5 (tGFP-tagged) - Human guanine nucleotide binding protein (G protein), beta 5 (GNB5), transcript variant 1
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_006578" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | GNB5 |
Synonyms | GB5; gbeta5; IDDCA; LADCI |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG204134 representing NM_006578
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAACCGAGGGGCTGCACGAGAACGAGACGCTGGCGTCGCTGAAGAGCGAGGCCGAGAGCCTCAAGG GCAAGCTGGAGGAGGAGCGAGCCAAGCTGCACGATGTGGAGCTGCACCAGGTGGCGGAGCGGGTGGAGGC CCTGGGGCAGTTTGTCATGAAGACCAGAAGGACCCTCAAAGGCCACGGGAACAAAGTCCTGTGCATGGAC TGGTGCAAAGATAAGAGGAGGATCGTGAGCTCGTCACAGGATGGGAAGGTGATCGTGTGGGATTCCTTCA CCACAAACAAGGAGCACGCGGTCACCATGCCCTGCACGTGGGTGATGGCATGTGCTTATGCCCCATCGGG ATGTGCCATTGCTTGTGGTGGTTTGGATAATAAGTGTTCTGTGTACCCCTTGACGTTTGACAAAAATGAA AACATGGCTGCCAAAAAGAAGTCTGTTGCTATGCACACCAACTACCTGTCGGCCTGCAGCTTCACCAACT CTGACATGCAGATCCTGACAGCGAGCGGCGATGGCACATGTGCCCTGTGGGACGTGGAGAGCGGGCAGCT GCTGCAGAGCTTCCACGGACATGGGGCTGACGTCCTCTGCTTGGACCTGGCCCCCTCAGAAACTGGAAAC ACCTTCGTGTCTGGGGGATGTGACAAGAAAGCCATGGTGTGGGACATGCGCTCCGGCCAGTGCGTGCAGG CCTTTGAAACACATGAATCTGACATCAACAGTGTCCGGTACTACCCCAGTGGAGATGCCTTTGCTTCAGG GTCAGATGACGCTACGTGTCGCCTCTATGACCTGCGGGCAGATAGGGAGGTTGCCATCTATTCCAAAGAA AGCATCATATTTGGAGCATCCAGCGTGGACTTCTCCCTCAGTGGTCGCCTGCTGTTTGCTGGATACAATG ATTACACTATCAACGTCTGGGATGTTCTCAAAGGGTCCCGGGTCTCCATCCTGTTTGGACATGAAAACCG CGTTAGCACTCTACGAGTTTCCCCCGATGGGACTGCTTTCTGCTCTGGATCATGGGATCATACCCTCAGA GTCTGGGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204134 representing NM_006578
Red=Cloning site Green=Tags(s) MATEGLHENETLASLKSEAESLKGKLEEERAKLHDVELHQVAERVEALGQFVMKTRRTLKGHGNKVLCMD WCKDKRRIVSSSQDGKVIVWDSFTTNKEHAVTMPCTWVMACAYAPSGCAIACGGLDNKCSVYPLTFDKNE NMAAKKKSVAMHTNYLSACSFTNSDMQILTASGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGN TFVSGGCDKKAMVWDMRSGQCVQAFETHESDINSVRYYPSGDAFASGSDDATCRLYDLRADREVAIYSKE SIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGSRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLR VWA TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_006578 |
ORF Size | 1059 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_006578.4 |
RefSeq Size | 2979 bp |
RefSeq ORF | 1062 bp |
Locus ID | 10681 |
UniProt ID | O14775 |
Cytogenetics | 15q21.2 |
Domains | WD40 |
Protein Families | Druggable Genome |
Protein Pathways | Chemokine signaling pathway |
Gene Summary | Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternatively spliced transcript variants encoding different isoforms exist. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204134 | GNB5 (Myc-DDK-tagged)-Human guanine nucleotide binding protein (G protein), beta 5 (GNB5), transcript variant 1 |
USD 457.00 |
|
RC204134L3 | Lenti ORF clone of Human guanine nucleotide binding protein (G protein), beta 5 (GNB5), transcript variant 1, Myc-DDK-tagged |
USD 757.00 |
|
RC204134L4 | Lenti ORF clone of Human guanine nucleotide binding protein (G protein), beta 5 (GNB5), transcript variant 1, mGFP tagged |
USD 757.00 |
|
SC321446 | GNB5 (untagged)-Human guanine nucleotide binding protein (G protein), beta 5 (GNB5), transcript variant 1 |
USD 457.00 |
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