SNF2H (SMARCA5) (NM_003601) Human Tagged ORF Clone
CAT#: RG203775
- TrueORF®
SMARCA5 (tGFP-tagged) - Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 (SMARCA5)
ORF Plasmid: DDK
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
"NM_003601" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | SNF2H |
Synonyms | hISWI; hSNF2H; ISWI; SNF2H; WCRF135 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG203775 representing NM_003601
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTCCGCGGCCGAGCCTCCGCCACCCCCGCCTCCCGAGAGCGCGCCTTCCAAGCCCGCAGCCTCGA TCGCCAGCGGCGGGAGCAACAGCAGCAACAAAGGCGGCCCCGAAGGCGTCGCGGCGCAGGCGGTTGCGTC TGCGGCCAGCGCTGGTCCCGCAGACGCCGAGATGGAGGAAATATTTGATGATGCGTCACCTGGAAAGCAA AAGGAAATCCAAGAACCAGATCCTACCTATGAAGAAAAAATGCAAACTGACCGGGCAAATAGATTCGAGT ATTTATTAAAGCAGACAGAACTTTTTGCACATTTCATTCAACCTGCTGCTCAGAAGACTCCAACTTCACC TTTGAAGATGAAACCAGGGCGCCCACGAATAAAAAAAGATGAGAAGCAGAACTTACTATCCGTTGGCGAT TACCGACACCGTAGAACAGAGCAAGAGGAGGATGAAGAGCTATTAACAGAAAGCTCCAAAGCAACCAATG TTTGCACTCGATTTGAAGACTCTCCATCGTATGTAAAATGGGGTAAACTGAGAGATTATCAGGTCCGAGG ATTAAACTGGCTCATTTCTTTGTATGAGAATGGCATCAATGGTATCCTTGCAGATGAAATGGGCCTAGGA AAGACTCTTCAAACAATTTCTCTTCTTGGGTACATGAAACATTATAGAAACATTCCTGGGCCTCATATGG TTTTGGTTCCTAAGTCTACATTACACAACTGGATGAGTGAATTCAAGAGATGGGTACCAACACTTAGATC TGTTTGTTTGATAGGAGATAAAGAACAAAGAGCTGCTTTTGTCAGAGACGTTTTATTACCGGGAGAATGG GATGTATGTGTAACATCTTATGAAATGCTTATTAAAGAGAAGTCTGTGTTCAAAAAATTTAATTGGAGAT ACTTAGTAATAGATGAAGCTCACAGGATCAAAAATGAAAAATCTAAGTTGTCAGAAATAGTGAGGGAATT CAAGACTACAAATAGACTATTATTAACTGGAACACCTCTTCAGAACAACTTGCATGAGCTGTGGTCACTT CTTAACTTTCTGTTGCCAGATGTGTTTAATTCAGCAGATGACTTTGATTCCTGGTTTGATACAAACAACT GCCTTGGGGATCAAAAACTAGTTGAGAGGCTTCATATGGTTTTGCGTCCATTCCTCCTTCGTCGAATTAA GGCTGATGTTGAAAAGAGTTTGCCTCCAAAGAAGGAAGTAAAAATCTATGTGGGCCTCAGCAAAATGCAA AGGGAATGGTATACTCGGATATTAATGAAGGATATAGATATACTCAACTCAGCAGGCAAGATGGACAAAA TGAGGTTATTGAACATCCTAATGCAGTTGAGAAAATGTTGTAATCATCCATATCTCTTTGATGGAGCAGA ACCTGGTCCACCTTATACAACAGATATGCATCTAGTAACCAACAGTGGCAAAATGGTGGTTTTAGACAAG CTGCTCCCTAAGTTAAAAGAACAAGGTTCACGAGTACTAATCTTCAGTCAAATGACAAGGGTATTGGACA TTTTGGAAGATTATTGCATGTGGAGAAATTATGAGTACTGCAGGTTGGATGGTCAGACACCCCATGATGA GAGACAAGACTCCATCAATGCATACAATGAACCAAACAGCACAAAGTTTGTTTTCATGTTAAGCACGCGT GCTGGTGGTCTTGGCATCAATCTTGCGACTGCTGATGTAGTAATTTTGTATGATTCTGATTGGAATCCCC AAGTAGATCTTCAGGCTATGGACCGAGCACATAGAATTGGGCAGACTAAGACAGTCAGAGTGTTCCGCTT TATAACTGATAACACTGTAGAAGAAAGAATAGTAGAACGTGCTGAGATGAAACTCAGACTGGATTCAATA GTCATTCAACAAGGGAGGCTTGTGGATCAGAATCTGAACAAAATTGGGAAAGATGAAATGCTTCAAATGA TTAGACATGGAGCAACACATGTGTTTGCTTCAAAGGAAAGTGAGATCACTGATGAAGATATCGATGGTAT TTTGGAAAGAGGTGCAAAGAAGACTGCAGAGATGAATGAAAAGCTCTCCAAGATGGGCGAAAGTTCACTT AGAAACTTTACAATGGATACAGAGTCAAGTGTTTATAACTTCGAAGGAGAAGACTATAGAGAAAAACAAA AGATTGCATTCACAGAGTGGATTGAACCACCTAAACGAGAAAGAAAAGCCAACTATGCCGTTGATGCATA TTTCAGGGAAGCTCTTCGTGTTAGTGAACCTAAAGCACCCAAGGCTCCTCGACCTCCAAAACAACCCAAT GTTCAGGATTTCCAGTTCTTTCCTCCACGTTTATTTGAATTACTGGAAAAAGAAATTCTGTTTTACAGAA AAACTATTGGGTACAAGGTACCTCGAAATCCTGAGCTGCCTAACGCAGCACAGGCACAAAAAGAAGAACA GCTTAAAATTGATGAAGCTGAATCCCTTAATGATGAAGAGTTAGAGGAAAAAGAGAAGCTTCTAACACAG GGATTTACCAATTGGAATAAGAGAGATTTTAACCAGTTTATCAAAGCTAATGAGAAGTGGGGTCGTGATG ATATTGAAAATATAGCAAGAGAAGTAGAAGGCAAAACTCCAGAAGAAGTCATTGAATATTCAGCTGTGTT TTGGGAAAGGTGCAACGAGCTCCAGGACATAGAGAAGATTATGGCTCAGATTGAAAGGGGAGAGGCGAGA ATTCAAAGAAGAATAAGCATCAAGAAAGCACTTGACACAAAGATTGGACGGTACAAAGCACCTTTTCATC AGCTGAGAATATCATATGGTACTAACAAAGGAAAAAACTATACTGAAGAAGAAGATCGTTTTCTGATTTG TATGCTTCACAAACTTGGATTTGACAAAGAAAATGTTTATGATGAATTGCGACAGTGTATTCGCAACTCT CCTCAGTTCAGATTTGACTGGTTTCTTAAGTCCAGAACTGCAATGGAGCTCCAGAGGAGATGTAATACCT TAATTACTTTGATTGAAAGAGAAAACATGGAACTAGAAGAAAAGGAGAAGGCAGAGAAAAAGAAACGAGG ACCAAAGCCTTCAACACAGAAACGTAAAATGGATGGCGCACCTGATGGTCGAGGAAGAAAAAAGAAGCTG AAACTA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203775 representing NM_003601
Red=Cloning site Green=Tags(s) MSSAAEPPPPPPPESAPSKPAASIASGGSNSSNKGGPEGVAAQAVASAASAGPADAEMEEIFDDASPGKQ KEIQEPDPTYEEKMQTDRANRFEYLLKQTELFAHFIQPAAQKTPTSPLKMKPGRPRIKKDEKQNLLSVGD YRHRRTEQEEDEELLTESSKATNVCTRFEDSPSYVKWGKLRDYQVRGLNWLISLYENGINGILADEMGLG KTLQTISLLGYMKHYRNIPGPHMVLVPKSTLHNWMSEFKRWVPTLRSVCLIGDKEQRAAFVRDVLLPGEW DVCVTSYEMLIKEKSVFKKFNWRYLVIDEAHRIKNEKSKLSEIVREFKTTNRLLLTGTPLQNNLHELWSL LNFLLPDVFNSADDFDSWFDTNNCLGDQKLVERLHMVLRPFLLRRIKADVEKSLPPKKEVKIYVGLSKMQ REWYTRILMKDIDILNSAGKMDKMRLLNILMQLRKCCNHPYLFDGAEPGPPYTTDMHLVTNSGKMVVLDK LLPKLKEQGSRVLIFSQMTRVLDILEDYCMWRNYEYCRLDGQTPHDERQDSINAYNEPNSTKFVFMLSTR AGGLGINLATADVVILYDSDWNPQVDLQAMDRAHRIGQTKTVRVFRFITDNTVEERIVERAEMKLRLDSI VIQQGRLVDQNLNKIGKDEMLQMIRHGATHVFASKESEITDEDIDGILERGAKKTAEMNEKLSKMGESSL RNFTMDTESSVYNFEGEDYREKQKIAFTEWIEPPKRERKANYAVDAYFREALRVSEPKAPKAPRPPKQPN VQDFQFFPPRLFELLEKEILFYRKTIGYKVPRNPELPNAAQAQKEEQLKIDEAESLNDEELEEKEKLLTQ GFTNWNKRDFNQFIKANEKWGRDDIENIAREVEGKTPEEVIEYSAVFWERCNELQDIEKIMAQIERGEAR IQRRISIKKALDTKIGRYKAPFHQLRISYGTNKGKNYTEEEDRFLICMLHKLGFDKENVYDELRQCIRNS PQFRFDWFLKSRTAMELQRRCNTLITLIERENMELEEKEKAEKKKRGPKPSTQKRKMDGAPDGRGRKKKL KL SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_003601 |
ORF Size | 3156 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003601.3 |
RefSeq Size | 3866 bp |
RefSeq ORF | 3159 bp |
Locus ID | 8467 |
UniProt ID | O60264 |
Cytogenetics | 4q31.21 |
Domains | SNF2_N, myb_DNA-binding, DEAD, helicase_C |
Protein Families | Transcription Factors |
Gene Summary | The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203775 | SMARCA5 (Myc-DDK-tagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 (SMARCA5) |
USD 916.00 |
|
RC203775L1 | Lenti ORF clone of Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 (SMARCA5), Myc-DDK-tagged |
USD 1,216.00 |
|
RC203775L2 | Lenti ORF clone of Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 (SMARCA5), mGFP tagged |
USD 1,216.00 |
|
RC203775L3 | Lenti ORF clone of Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 (SMARCA5), Myc-DDK-tagged |
USD 1,216.00 |
|
RC203775L4 | Lenti ORF clone of Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 (SMARCA5), mGFP tagged |
USD 1,216.00 |
|
SC126702 | SMARCA5 (untagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 (SMARCA5) |
USD 917.00 |
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