PHF1 (NM_024165) Human Tagged ORF Clone
CAT#: RG202906
- TrueORF®
PHF1 (tGFP-tagged) - Human PHD finger protein 1 (PHF1), transcript variant 2
ORF Plasmid: DDK
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
"NM_024165" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | PHF1 |
Synonyms | hPHF1; MTF2L2; PCL1; PHF2; TDRD19C |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG202906 representing NM_024165
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGGCGCGCCC ATGGCGCAGCCCCCCCGGCTGAGCCGCTCTGGTGCCTCCTCACTTTGGGACCCAGCTTCTCCTGCTCCCA CCTCTGGCCCCAGGCCTCGGCTTTGGGAGGGTCAAGATGTGCTGGCCAGATGGACTGATGGGCTGCTATA CTTGGGTACCATCAAAAAGGTGGACAGTGCTAGGGAGGTGTGTCTGGTCCAGTTTGAGGATGATTCGCAG TTTCTGGTTCTATGGAAAGACATTAGCCCTGCTGCCCTCCCTGGAGAGGAACTCCTCTGTTGTGTCTGTC GCTCTGAGACTGTGGTCCCTGGGAACCGGCTGGTCAGCTGTGAGAAGTGTCGCCATGCTTATCACCAGGA CTGCCATGTTCCCAGGGCTCCAGCCCCTGGAGAGGGAGAGGGCACATCCTGGGTATGCCGCCAGTGTGTC TTTGCGATCGCCACCAAGAGGGGAGGTGCCCTGAAGAAGGGCCCCTATGCCCGGGCCATGCTGGGTATGA AGCTTTCTCTGCCATATGGACTGAAGGGGCTGGACTGGGATGCTGGACATCTGAGCAACCGACAGCAGAG TTACTGTTACTGTGGTGGCCCTGGGGAGTGGAACCTGAAAATGCTGCAGTGCCGGAGCTGCCTGCAGTGG TTCCATGAGGCCTGCACCCAGTGTCTGAGCAAGCCCCTCCTCTATGGGGACAGGTTCTATGAATTTGAAT GCTGTGTGTGTCGCGGGGGCCCTGAGAAAGTCCGGAGACTACAGCTTCGCTGGGTGGATGTGGCCCATCT TGTCCTGTATCACCTCAGTGTTTGCTGTAAGAAGAAATACTTTGATTTTGATCGTGAGATCCTCCCCTTC ACTTCTGAGAATTGGGACAGTTTGCTCCTGGGGGAGCTTTCAGACACCCCCAAAGGAGAACGTTCTTCCA AGCTCCTCTCTGCTCTTAACAGCCACAAGGACCGTTTCATTTCAGGGAGAGAGATTAAGAAGAGGAAATG TTTGTTTGGTCTCCATGCTCGGATGCCTCCCCCTGTGGAGCCCCCTACTGGAGATGGAGCACTCACCAGC TTCCCTTCAGGGCAGGGCCCTGGGGGAGGGGTCTCACGTCCCCTGGGGAAGCGCCGGAGGCCGGAGCCAG AGCCCCTGAGGAGGAGGCAGAAGGGGAAAGTGGAGGAGCTGGGGCCACCCTCAGCAGTGCGCAATCAGCC CGAGCCCCAGGAGCAGAGGGAGCGGGCTCATCTGCAGAGGGCACTGCAGGCCTCAGTGTCTCCACCATCC CCCAGCCCTAACCAGAGTTACCAGGGCAGCAGCGGCTACAACTTCCGGCCCACAGATGCCCGCTGCCTGC CCAGCAGCCCCATCCGGATGTTTGCTTCCTTCCACCCTTCTGCCAGCACCGCAGGGACCTCTGGGGACAG TGGACCCCCAGACAGGTCACCCCTGGAACTTCACATTGGTTTCCCCACAGACATCCCTAAAAGTGCCCCC CACTCGATGACTGCCTCATCTTCCTCAGTTTCATCCCCATCCCCAGGTCTTCCTAGACGCTCAGCACCCC CTTCTCCCCTGTGCCGTAGTTTGTCTCCTGGGACTGGGGGAGGAGTCCGAGGTGGGGTTGGTTACCTGTC CCGAGGGGACCCTGTCCGGGTCCTTGCTCGGAGAGTACGGCCTGATGGCTCTGTGCAGTACCTGGTTGAG TGGGGAGGAGGGGGCATCTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG202906 representing NM_024165
Red=Cloning site Green=Tags(s) MAQPPRLSRSGASSLWDPASPAPTSGPRPRLWEGQDVLARWTDGLLYLGTIKKVDSAREVCLVQFEDDSQ FLVLWKDISPAALPGEELLCCVCRSETVVPGNRLVSCEKCRHAYHQDCHVPRAPAPGEGEGTSWVCRQCV FAIATKRGGALKKGPYARAMLGMKLSLPYGLKGLDWDAGHLSNRQQSYCYCGGPGEWNLKMLQCRSCLQW FHEACTQCLSKPLLYGDRFYEFECCVCRGGPEKVRRLQLRWVDVAHLVLYHLSVCCKKKYFDFDREILPF TSENWDSLLLGELSDTPKGERSSKLLSALNSHKDRFISGREIKKRKCLFGLHARMPPPVEPPTGDGALTS FPSGQGPGGGVSRPLGKRRRPEPEPLRRRQKGKVEELGPPSAVRNQPEPQEQRERAHLQRALQASVSPPS PSPNQSYQGSSGYNFRPTDARCLPSSPIRMFASFHPSASTAGTSGDSGPPDRSPLELHIGFPTDIPKSAP HSMTASSSSVSSPSPGLPRRSAPPSPLCRSLSPGTGGGVRGGVGYLSRGDPVRVLARRVRPDGSVQYLVE WGGGGIF TRTRPLE - GFP Tag - V |
Restriction Sites |
AscI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_024165 |
ORF Size | 1701 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024165.2 |
RefSeq Size | 2260 bp |
RefSeq ORF | 1704 bp |
Locus ID | 5252 |
UniProt ID | O43189 |
Cytogenetics | 6p21.32 |
Domains | PHD, TUDOR |
Protein Families | Druggable Genome, Transcription Factors |
Gene Summary | This gene encodes a Polycomb group protein. The protein is a component of a histone H3 lysine-27 (H3K27)-specific methyltransferase complex, and functions in transcriptional repression of homeotic genes. The protein is also recruited to double-strand breaks, and reduced protein levels results in X-ray sensitivity and increased homologous recombination. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202906 | PHF1 (Myc-DDK-tagged)-Human PHD finger protein 1 (PHF1), transcript variant 2 |
USD 528.00 |
|
RC202906L1 | Lenti ORF clone of Human PHD finger protein 1 (PHF1), transcript variant 2, Myc-DDK-tagged |
USD 828.00 |
|
RC202906L2 | Lenti ORF clone of Human PHD finger protein 1 (PHF1), transcript variant 2, mGFP tagged |
USD 828.00 |
|
RC202906L3 | Lenti ORF clone of Human PHD finger protein 1 (PHF1), transcript variant 2, Myc-DDK-tagged |
USD 828.00 |
|
RC202906L4 | Lenti ORF clone of Human PHD finger protein 1 (PHF1), transcript variant 2, mGFP tagged |
USD 828.00 |
|
SC112257 | PHF1 (untagged)-Human PHD finger protein 1 (PHF1), transcript variant 2 |
USD 529.00 |
|
SC320779 | PHF1 (untagged)-Human PHD finger protein 1 (PHF1), transcript variant 2 |
USD 529.00 |
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