MCC (NM_002387) Human Tagged ORF Clone

CAT#: RG202890

MCC (tGFP-tagged) - Human mutated in colorectal cancers (MCC), transcript variant 2

Product Images

Circular map for RG202890

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MCC
• Synonyms: MCC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202890 representing NM_002387
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTCCGGAGTTGCCATGAAATATGGAAACGACTCCTCGGCCGAGCTGAGTGAGCTCCATTCAGCAG
  CCCTGGCATCACTAAAGGGAGATATAGTGGAACTTAATAAACGTCTCCAGCAAACAGAGAGGGAACGGGA
  CCTTCTGGAAAAGAAATTGGCCAAGGCACAGTGCGAGCAGTCCCACCTCATGAGAGAGCATGAGGATGTC
  CAGGAGCGAACGACGCTTCGCTATGAGGAACGCATCACAGAGCTCCACAGCGTCATTGCGGAGCTCAACA
  AGAAGATAGACCGTCTGCAAGGCACCACCATCAGGGAGGAAGATGAGTACTCAGAACTGCGATCAGAACT
  CAGCCAGAGCCAACACGAGGTCAACGAGGACTCTCGAAGCATGGACCAAGACCAGACCTCTGTCTCTATC
  CCCGAAAACCAGTCTACCATGGTTACTGCTGACATGGACAACTGCAGTGACCTGAACTCAGAACTGCAGA
  GGGTGCTGACAGGGCTGGAGAATGTTGTCTGCGGCAGGAAGAAGAGCAGCTGCAGCCTCTCCGTGGCCGA
  GGTGGACAGGCACATTGAGCAGCTCACCACAGCCAGCGAGCACTGTGACCTGGCTATTAAGACAGTCGAG
  GAGATTGAGGGGGTGCTTGGCCGGGACCTGTATCCCAACCTGGCTGAAGAGAGGTCTCGGTGGGAGAAGG
  AGCTGGCTGGGCTGAGGGAAGAGAATGAGAGCCTGACTGCCATGCTGTGCAGCAAAGAGGAAGAACTGAA
  CCGGACTAAGGCCACCATGAATGCCATCCGGGAAGAGCGGGACCGGCTCCGGAGGAGGGTCAGAGAGCTT
  CAAACTCGACTACAGAGCGTGCAGGCCACAGGTCCCTCCAGCCCTGGCCGCCTCACTTCCACCAACCGCC
  CGATTAACCCCAGCACTGGGGAGCTGAGCACAAGCAGCAGCAGCAATGACATTCCCATCGCCAAGATTGC
  TGAGAGGGTGAAGCTATCAAAGACAAGGTCCGAATCGTCATCATCTGATCGGCCAGTCCTGGGCTCAGAA
  ATCAGTAGCATAGGGGTATCCAGCAGTGTGGCTGAACACCTGGCCCACTCACTTCAGGACTGCTCCAATA
  TCCAAGAGATTTTCCAAACACTCTACTCACACGGATCTGCCATCTCAGAAAGCAAGATTAGAGAGTTTGA
  GGTGGAAACAGAACGGCTGAATAGCCGGATTGAGCACCTCAAATCCCAAAATGACCTCCTGACCATAACC
  TTGGAGGAATGTAAAAGCAATGCCGAGAGGATGAGCATGCTGGTGGGAAAATACGAATCCAATGCCACAG
  CGCTGAGGCTGGCCTTGCAGTACAGCGAGCAGTGCATCGAAGCCTACGAACTCCTCCTGGCGCTGGCAGA
  GAGTGAGCAGAGCCTCATCCTGGGGCAGTTCCGAGCGGCGGGCGTGGGGTCCTCCCCTGGAGACCAGTCG
  GGGGATGAAAACATCACTCAGATGCTCAAGCGAGCTCATGACTGCCGGAAGACAGCTGAGAATGCTGCCA
  AGGCCCTGCTCATGAAGCTGGACGGCAGCTGTGGGGGAGCCTTTGCCGTGGCCGGCTGCAGCGTGCAGCC
  CTGGGAGAGCCTTTCCTCCAACAGCCACACCAGCACAACCAGCTCCACAGCCAGTAGTTGCGACACCGAG
  TTCACTAAAGAAGACGAGCAGAGGCTGAAGGATTATATCCAGCAGCTCAAGAATGACAGGGCTGCGGTCA
  AGCTGACCATGCTGGAGCTGGAAAGCATCCACATCGATCCTCTCAGCTATGACGTCAAGCCTCGGGGAGA
  CAGCCAGAGGCTGGATCTGGAAAACGCAGTGCTTATGCAGGAGCTCATGGCCATGAAGGAGGAGATGGCC
  GAGTTGAAGGCCCAGCTCTACCTACTGGAGAAAGAGAAGAAGGCCCTGGAGCTGAAGCTGAGCACGCGGG
  AGGCCCAGGAGCAGGCCTACCTGGTGCACATTGAGCACCTGAAGTCCGAGGTGGAGGAGCAGAAGGAGCA
  GCGGATGCGATCCCTCAGCTCCACCAGCAGCGGCAGCAAAGATAAACCTGGCAAGGAGTGTGCTGATGCT
  GCCTCCCCAGCTCTGTCCCTAGCTGAACTCAGGACAACGTGCAGCGAGAATGAGCTGGCTGCGGAGTTCA
  CCAACGCCATTCGTCGAGAAAAGAAGTTGAAGGCCAGAGTTCAAGAGCTGGTGAGTGCCTTGGAGAGACT
  CACCAAGAGCAGTGAAATCCGACATCAGCAATCTGCAGAGTTCGTGAATGATCTAAAGCGGGCCAACAGC
  AACCTGGTGGCTGCCTATGAGAAAGCAAAGAAAAAGCATCAAAACAAACTGAAGAAGTTAGAGTCGCAGA
  TGATGGCCATGGTGGAGAGACATGAGACCCAAGTGAGGATGCTCAAGCAAAGAATAGCTCTGCTAGAGGA
  GGAGAACTCCAGGCCACACACCAATGAAACTTCGCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202890 representing NM_002387
  Red=Cloning site Green=Tags(s)
  
  MNSGVAMKYGNDSSAELSELHSAALASLKGDIVELNKRLQQTERERDLLEKKLAKAQCEQSHLMREHEDV
  QERTTLRYEERITELHSVIAELNKKIDRLQGTTIREEDEYSELRSELSQSQHEVNEDSRSMDQDQTSVSI
  PENQSTMVTADMDNCSDLNSELQRVLTGLENVVCGRKKSSCSLSVAEVDRHIEQLTTASEHCDLAIKTVE
  EIEGVLGRDLYPNLAEERSRWEKELAGLREENESLTAMLCSKEEELNRTKATMNAIREERDRLRRRVREL
  QTRLQSVQATGPSSPGRLTSTNRPINPSTGELSTSSSSNDIPIAKIAERVKLSKTRSESSSSDRPVLGSE
  ISSIGVSSSVAEHLAHSLQDCSNIQEIFQTLYSHGSAISESKIREFEVETERLNSRIEHLKSQNDLLTIT
  LEECKSNAERMSMLVGKYESNATALRLALQYSEQCIEAYELLLALAESEQSLILGQFRAAGVGSSPGDQS
  GDENITQMLKRAHDCRKTAENAAKALLMKLDGSCGGAFAVAGCSVQPWESLSSNSHTSTTSSTASSCDTE
  FTKEDEQRLKDYIQQLKNDRAAVKLTMLELESIHIDPLSYDVKPRGDSQRLDLENAVLMQELMAMKEEMA
  ELKAQLYLLEKEKKALELKLSTREAQEQAYLVHIEHLKSEVEEQKEQRMRSLSSTSSGSKDKPGKECADA
  ASPALSLAELRTTCSENELAAEFTNAIRREKKLKARVQELVSALERLTKSSEIRHQQSAEFVNDLKRANS
  NLVAAYEKAKKKHQNKLKKLESQMMAMVERHETQVRMLKQRIALLEEENSRPHTNETSL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002387
• ORF Size: 2487 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002387.1, NP_002378.1
• RefSeq Size: 4181 bp
• RefSeq ORF: 2490 bp
• Locus ID: 4163
• UniProt ID: P23508
• Cytogenetics: 5q22.2
• Protein Families: Druggable Genome
• Gene Summary: This gene is a candidate colorectal tumor suppressor gene that is thought to negatively regulate cell cycle progression. The orthologous gene in the mouse expresses a phosphoprotein associated with the plasma membrane and membrane organelles, and overexpression of the mouse protein inhibits entry into S phase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]