PACC1 (NM_018252) Human Tagged ORF Clone

CAT#: RG202838

TMEM206 (tGFP-tagged) - Human transmembrane protein 206 (TMEM206), transcript variant 2

Product Images

Circular map for RG202838

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PACC1
• Synonyms: ASOR; C1orf75; hPAC; hTMEM206; PAC; PAORAC; TMEM206
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202838 representing NM_018252
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCGGCAGGAGCGCTCCACATCCTACCAGGAGCTGAGTGAGGAGTTGGTCCAGGTGGTTGAGAGCT
  CAGAGCTGGCAGACGAGCAGGACAAGGAGACGGTCAGAGTCCAAGGTCCGGGTATCTTACCAGGCCTGGA
  CAGCGAGTCCGCCTCCAGCAGCATCCGCTTCAGCAAGGCCTGCCTGAAGAACGTCTTCTCGGTCCTACTC
  ATCTTCATCTACCTGCTGCTCATGGCTGTGGCCGTCTTCCTGGTCTACCGGACCATCACAGACTTTCGTG
  AGAAACTCAAGCACCCTGTCATGTCTGTGTCTTACAAGGAAGTGGATCGCTATGATGCCCCAGGTATTGC
  CTTGTACCCCGGTCAGGCCCAGTTGCTCAGCTGTAAGCACCATTACGAGGTCATTCCTCCTCTGACAAGC
  CCTGGCCAGCCGGGTGACATGAATTGCACCACCCAGAGGATCAACTACACGGACCCCTTCTCCAATCAGA
  CTGTGAAATCTGCCCTGATTGTCCAGGGGCCCCGGGAAGTGAAAAAGCGGGAGCTGGTCTTCCTCCAGTT
  CCGCCTGAACAAGAGTAGTGAGGACTTCAGCGCCATTGATTACCTCCTCTTCTCTTCTTTCCAGGAGTTC
  CTGCAAAGCCCAAACAGGGTAGGCTTCATGCAGGCCTGTGAGAGTGCCTGTTCCAGCTGGAAGTTCTCTG
  GGGGCTTCCGCACCTGGGTCAAGATGTCACTGGTAAAGACCAAGGAGGAGGATGGGCGGGAAGCAGTGGA
  GTTCCGGCAGGAGACAAGTGTGGTTAACTACATTGACCAGAGGCCAGCTGCCAAAAAAAGTGCTCAATTG
  TTTTTTGTGGTCTTTGAATGGAAAGATCCTTTCATCCAGAAAGTCCAAGATATAGTCACTGCCAATCCTT
  GGAACACAATTGCTCTTCTCTGTGGCGCCTTCTTGGCATTATTTAAAGCAGCAGAGTTTGCCAAACTGAG
  TATAAAATGGATGATCAAAATTAGAAAGAGATACCTTAAAAGAAGAGGTCAGGCAACGAGCCACATAAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202838 representing NM_018252
  Red=Cloning site Green=Tags(s)
  
  MIRQERSTSYQELSEELVQVVESSELADEQDKETVRVQGPGILPGLDSESASSSIRFSKACLKNVFSVLL
  IFIYLLLMAVAVFLVYRTITDFREKLKHPVMSVSYKEVDRYDAPGIALYPGQAQLLSCKHHYEVIPPLTS
  PGQPGDMNCTTQRINYTDPFSNQTVKSALIVQGPREVKKRELVFLQFRLNKSSEDFSAIDYLLFSSFQEF
  LQSPNRVGFMQACESACSSWKFSGGFRTWVKMSLVKTKEEDGREAVEFRQETSVVNYIDQRPAAKKSAQL
  FFVVFEWKDPFIQKVQDIVTANPWNTIALLCGAFLALFKAAEFAKLSIKWMIKIRKRYLKRRGQATSHIS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018252
• ORF Size: 1050 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018252.1, NP_060722.1
• RefSeq Size: 1978 bp
• RefSeq ORF: 1053 bp
• Locus ID: 55248
• UniProt ID: Q9H813
• Cytogenetics: 1q32.3
• Protein Families: Transmembrane
• Gene Summary: Proton-activated chloride channel that mediates import of chloride ion in response to extracellular acidic pH (PubMed:31023925). Involved in acidosis-induced cell death by mediating chloride influx and subsequent cell swelling (PubMed:31023925).[UniProtKB/Swiss-Prot Function]