ALG13 (NM_018466) Human Tagged ORF Clone
CAT#: RG202762
- TrueORF®
ALG13 (tGFP-tagged) - Human asparagine-linked glycosylation 13 homolog (S. cerevisiae) (ALG13), transcript variant 2
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_018466" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | ALG13 |
Synonyms | CDG1S; CXorf45; DEE36; EIEE36; GLT28D1; MDS031; TDRD13; YGL047W |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG202762 representing NM_018466
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTGCGTGTTTGTTACCGTAGGGACCACCAGCTTTGACGACCTCATTGCGTGTGTGTCGGCGCCCG ACAGTCTGCAAAAAATCGAGAGCCTTGGTTACAACCGACTTATCCTGCAAATTGGTAGAGGAACGGTGGT ACCTGAACCCTTCAGTACTGAGTCGTTTACTCTGGATGTTTACAGGTACAAGGATTCCTTGAAAGAAGAC ATTCAGAAAGCAGATCTTGTTATTAGTCACGCAGGTGCAGGAAGCTGTTTGGAGACTCTGGAAAAAGGAA AGCCACTCGTAGTGGTTATAAACGAAAAGTTGATGAACAATCATCAGCTGGAACTGGCAAAGCAGCTACA CAAAGAGGGTCATCTCTTCTATTGTACCTGCAGCACGCTTCCTGGGCTGTTACAGTCAATGGACTTATCA ACACTGAAATGTTATCCTCCTGGCCAGCCAGAAAAATTTTCTGCATTTTTGGATAAAGTTGTTGGATTAC AAAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG202762 representing NM_018466
Red=Cloning site Green=Tags(s) MKCVFVTVGTTSFDDLIACVSAPDSLQKIESLGYNRLILQIGRGTVVPEPFSTESFTLDVYRYKDSLKED IQKADLVISHAGAGSCLETLEKGKPLVVVINEKLMNNHQLELAKQLHKEGHLFYCTCSTLPGLLQSMDLS TLKCYPPGQPEKFSAFLDKVVGLQK TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_018466 |
ORF Size | 495 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018466.6 |
RefSeq Size | 1358 bp |
RefSeq ORF | 498 bp |
Locus ID | 79868 |
UniProt ID | Q9NP73 |
Cytogenetics | Xq23 |
Domains | Glyco_tran_28_C |
Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
Gene Summary | The protein encoded by this gene is a subunit of a bipartite UDP-N-acetylglucosamine transferase. It heterodimerizes with asparagine-linked glycosylation 14 homolog to form a functional UDP-GlcNAc glycosyltransferase that catalyzes the second sugar addition of the highly conserved oligosaccharide precursor in endoplasmic reticulum N-linked glycosylation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202762 | ALG13 (Myc-DDK-tagged)-Human asparagine-linked glycosylation 13 homolog (S. cerevisiae) (ALG13), transcript variant 2 |
USD 150.00 |
|
RC202762L3 | Lenti ORF clone of Human asparagine-linked glycosylation 13 homolog (S. cerevisiae) (ALG13), transcript variant 2, Myc-DDK-tagged |
USD 450.00 |
|
RC202762L4 | Lenti ORF clone of Human asparagine-linked glycosylation 13 homolog (S. cerevisiae) (ALG13), transcript variant 2, mGFP tagged |
USD 450.00 |
|
SC324410 | ALG13 (untagged)-Human asparagine-linked glycosylation 13 homolog (S. cerevisiae) (ALG13), transcript variant 2 |
USD 150.00 |
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