CDK4 (NM_000075) Human Tagged ORF Clone
CAT#: RG201156
- TrueORF®
CDK4 (tGFP-tagged) - Human cyclin-dependent kinase 4 (CDK4)
ORF Plasmid: DDK
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
"NM_000075" in other vectors (9)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | CDK4 |
Synonyms | CMM3; PSK-J3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000075 |
ORF Size | 909 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000075.4 |
RefSeq Size | 1474 bp |
RefSeq ORF | 912 bp |
Locus ID | 1019 |
UniProt ID | P11802 |
Cytogenetics | 12q14.1 |
Domains | pkinase, TyrKc, S_TKc |
Protein Families | Druggable Genome, Protein Kinase |
Protein Pathways | Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Small cell lung cancer, T cell receptor signaling pathway, Tight junction |
Gene Summary | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201156 | CDK4 (Myc-DDK-tagged)-Human cyclin-dependent kinase 4 (CDK4) |
USD 450.00 |
|
RC201156L1 | Lenti ORF clone of Human cyclin-dependent kinase 4 (CDK4), Myc-DDK-tagged |
USD 750.00 |
|
RC201156L2 | Lenti ORF clone of Human cyclin-dependent kinase 4 (CDK4), mGFP tagged |
USD 750.00 |
|
RC201156L3 | Lenti ORF clone of Human cyclin-dependent kinase 4 (CDK4), Myc-DDK-tagged |
USD 750.00 |
|
RC201156L4 | Lenti ORF clone of Human cyclin-dependent kinase 4 (CDK4), mGFP tagged |
USD 750.00 |
|
SC112998 | CDK4 (untagged)-Human cyclin-dependent kinase 4 (CDK4) |
USD 450.00 |
|
SC323451 | CDK4 (untagged)-Kinase deficient mutant (K35M) of Human cyclin-dependent kinase 4 (CDK4) |
USD 450.00 |
|
SC323460 | CDK4 (untagged)-Kinase deficient mutant (K35M) of Human cyclin-dependent kinase 4 (CDK4) |
USD 450.00 |
|
SC323699 | CDK4 (untagged)-Kinase deficient mutant (K35M) of Human cyclin-dependent kinase 4 (CDK4) |
USD 450.00 |
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