CCK4 (PTK7) (NM_152882) Human Tagged ORF Clone
CAT#: RC600066
- TrueORF®
PTK7 (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens PTK7 protein tyrosine kinase 7, transcript variant PTK7-4, Signal peptide (1-30) plus EC domain (31-648)
"NM_152882" in other vectors (5)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | DDK-His |
Symbol | CCK4 |
Synonyms | CCK-4; CCK4 |
Vector | pCMV6-XL5-DDK-His |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>RC600066 representing leader sequence plus the extracellular domain region of NM_152882
Red=Cloning site Blue=ORF Green=Tags(s) GTAATACGACTCACTATAGGGCGGCCGCGAATTCGTCGACTGGATCTGGTACCGAGGAGATCCGCCGCCG CGATCGCC ATGGGAGCTGCGCGGGGATCCCCGGCCAGACCCCGCCGGTTGCCTCTGCTCAGCGTCCTGCTGCTGCCGC TGCTGGGCGGTACCCAGACAGCCATTGTCTTCATCAAGCAGCCGTCCTCCCAGGATGCACTGCAGGGGCG CCGGGCGCTGCTTCGCTGTGAGGTTGAGGCTCCGGGCCCGGTACATGTGTACTGGCTGCTCGATGGGGCC CCTGTCCAGGACACGGAGCGGCGTTTCGCCCAGGGCAGCAGCCTGAGCTTTGCAGCTGTGGACCGGCTGC AGGACTCTGGCACCTTCCAGTGTGTGGCTCGGGATGATGTCACTGGAGAAGAAGCCCGCAGTGCCAACGC CTCCTTCAACATCAAATGGATTGAGGCAGGTCCTGTGGTCCTGAAGCATCCAGCCTCGGAAGCTGAGATC CAGCCACAGACCCAGGTCACACTTCGTTGCCACATTGATGGGCACCCTCGGCCCACCTACCAATGGTTCC GAGATGGGACCCCCCTTTCTGATGGTCAGAGCAACCACACAGTCAGCAGCAAGGAGCGGAACCTGACGCT CCGGCCAGCTGGTCCTGAGCATAGTGGGCTGTATTCCTGCTGCGCCCACAGTGCTTTTGGCCAGGCTTGC AGCAGCCAGAACTTCACCTTGAGCATTGCTGATGAAAGCTTTGCCAGGGTGGTGCTGGCACCCCAGGACG TGGTAGTAGCGAGGTATGAGGAGGCCATGTTCCATTGCCAGTTCTCAGCCCAGCCACCCCCGAGCCTGCA GTGGCTCTTTGAGGATGAGACTCCCATCACTAACCGCAGTCGCCCCCCACACCTCCGCAGAGCCACAGTG TTTGCCAACGGGTCTCTGCTGCTGACCCAGGTCCGGCCACGCAATGCAGGGATCTACCGCTGCATTGGCC AGGGGCAGAGGGGCCCACCCATCATCCTGGAAGCCACACTTCACCTAGCAGAGATTGAAGACATGCCGCT ATTTGAGCCACGGGTGTTTACAGCTGGCAGCGAGGAGCGTGTGACCTGCCTTCCCCCCAAGGGTCTGCCA GAGCCCAGCGTGTGGTGGGAGCACGCGGGAGTCCGGCTGCCCACCCATGGCAGGGTCTACCAGAAGGGCC ACGAGCTGGTGTTGGCCAATATTGCTGAAAGTGATGCTGGTGTCTACACCTGCCACGCGGCCAACCTGGC TGGTCAGCGGAGACAGGATGTCAACATCACTGTGGCCACTGTGCCCTCCTGGCTGAAGAAGCCCCAAGAC AGCCAGCTGGAGGAGGGCAAACCCGGCTACTTGGATTGCCTGACCCAGGCCACACCAAAACCTACAGTTG TCTGGTACAGAAACCAGATGCTCATCTCAGAGGACTCACGGTTCGAGGTCTTCAAGAATGGGACCTTGCG CATCAACAGCGTGGAGGTGTATGATGGGACATGGTACCGTTGTATGAGCAGCACCCCAGCCGGCAGCATC GAGGCGCAAGCCCGTGTCCAAGTGCTGGAAAAGCTCAAGTTCACACCACCACCCCAGCCACAGCAGTGCA TGGAGTTTGACAAGGAGGCCACGGTGCCCTGTTCAGCCACAGGCCGAGAGAAGCCCACTATTAAGTGGGA ACGGGCAGATGGGAGCAGCCTCCCAGAGTGGGTGACAGACAACGCTGGGACCCTGCATTTTGCCCGGGTG ACTCGAGATGACGCTGGCAACTACACTTGCATTGCCTCCAACGGGCCGCAGGGCCAGATTCGTGCCCATG TCCAGCTCACTGTGGCAGTTTTTATCACCTTCAAAGTGGAACCAGAGCGTACGACTGTGTACCAGGGCCA CACAGCCCTACTGCAGTGCGAGGCCCAGGGGGACCCCAAGCCGCTGATTCAGTGGAAAGACAAGCCTGTG CCGGAGGAGTCGGAGGGCCCTGGCAGCCCTCCCCCCTACAAGATGATCCAGACC ACGCGTTCAGGCGACTACAAGGATGACGACGATAAGGGATCTCATCATCACCATCACCATTAATGAGATC TGGTACCGATATCAAGCTTGTCGACTCTAGA >RC600066 representing signal peptide plus the extracellular domain region of NM_152882
Red=Cloning sites Green= DDK and 6XHIS Tags MGAARGSPARPRRLPLLSVLLLPLLGGTQTAIVFIKQPSSQDALQGRRALLRCEVEAPGPVHVYWLLDGA PVQDTERRFAQGSSLSFAAVDRLQDSGTFQCVARDDVTGEEARSANASFNIKWIEAGPVVLKHPASEAEI QPQTQVTLRCHIDGHPRPTYQWFRDGTPLSDGQSNHTVSSKERNLTLRPAGPEHSGLYSCCAHSAFGQAC SSQNFTLSIADESFARVVLAPQDVVVARYEEAMFHCQFSAQPPPSLQWLFEDETPITNRSRPPHLRRATV FANGSLLLTQVRPRNAGIYRCIGQGQRGPPIILEATLHLAEIEDMPLFEPRVFTAGSEERVTCLPPKGLP EPSVWWEHAGVRLPTHGRVYQKGHELVLANIAESDAGVYTCHAANLAGQRRQDVNITVATVPSWLKKPQD SQLEEGKPGYLDCLTQATPKPTVVWYRNQMLISEDSRFEVFKNGTLRINSVEVYDGTWYRCMSSTPAGSI EAQARVQVLEKLKFTPPPQPQQCMEFDKEATVPCSATGREKPTIKWERADGSSLPEWVTDNAGTLHFARV TRDDAGNYTCIASNGPQGQIRAHVQLTVAVFITFKVEPERTTVYQGHTALLQCEAQGDPKPLIQWKDKPV PEESEGPGSPPPYKMIQT TRSGTRSGDYKDDDDKGSHHHHHH |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_152882 |
ORF Size | 1944 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the extra cellular domain of the protein with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_152882.3, NP_690621.1 |
RefSeq Size | 4105 bp |
RefSeq ORF | 3045 bp |
Locus ID | 5754 |
UniProt ID | Q13308 |
Cytogenetics | 6p21.1 |
Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
MW | 71.6 kDa |
Gene Summary | This gene encodes a member of the receptor protein tyrosine kinase family of proteins that transduce extracellular signals across the cell membrane. The encoded protein lacks detectable catalytic tyrosine kinase activity, is involved in the Wnt signaling pathway and plays a role in multiple cellular processes including polarity and adhesion. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223467 | PTK7 (Myc-DDK-tagged)-Human PTK7 protein tyrosine kinase 7 (PTK7), transcript variant PTK7-4 |
USD 971.00 |
|
RC223467L3 | Lenti-ORF clone of PTK7 (Myc-DDK-tagged)-Human PTK7 protein tyrosine kinase 7 (PTK7), transcript variant PTK7-4 |
USD 1,271.00 |
|
RC223467L4 | Lenti-ORF clone of PTK7 (mGFP-tagged)-Human PTK7 protein tyrosine kinase 7 (PTK7), transcript variant PTK7-4 |
USD 1,271.00 |
|
RG223467 | PTK7 (tGFP-tagged) - Human PTK7 protein tyrosine kinase 7 (PTK7), transcript variant PTK7-4 |
USD 1,171.00 |
|
SC309551 | PTK7 (untagged)-Human PTK7 protein tyrosine kinase 7 (PTK7), transcript variant PTK7-4 |
USD 972.00 |
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