KIF20B (NM_001284259) Human Tagged ORF Clone

CAT#: RC240214

KIF20B (myc-DDK-tagged) - Human kinesin family member 20B (KIF20B), transcript variant 1

Product Images

Circular map for RC240214

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KIF20B
• Synonyms: CT90; KRMP1; MPHOSPH1; MPP-1; MPP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC240214 representing NM_001284259
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATCTAATTTTAATCAAGAGGGAGTACCTCGACCATCTTATGTTTTTAGTGCTGACCCAATTGCAA
  GGCCTTCAGAAATAAATTTCGATGGCATTAAGCTTGATCTGTCTCATGAATTTTCCTTAGTTGCTCCAAA
  TACTGAGGCAAACAGTTTCGAATCTAAAGATTATCTCCAGGTTTGTCTTCGAATAAGACCATTTACACAG
  TCAGAAAAAGAACTTGAGTCTGAGGGCTGTGTGCATATTCTGGATTCACAGACTGTTGTGCTGAAAGAGC
  CTCAATGCATCCTTGGTCGGTTAAGTGAAAAAAGCTCAGGGCAGATGGCACAGAAATTCAGTTTTTCCAA
  GGTTTTTGGCCCAGCAACTACACAGAAGGAATTCTTTCAGGGTTGCATTATGCAACCAGTAAAAGACCTC
  TTGAAAGGACAGAGTCGTCTGATTTTTACTTACGGGCTAACCAATTCAGGAAAAACATATACATTTCAAG
  GGACAGAAGAAAATATTGGCATTCTGCCTCGAACTTTGAATGTATTATTTGATAGTCTTCAAGAAAGACT
  GTATACAAAGATGAACCTTAAACCACATAGATCCAGAGAATACTTAAGGTTATCATCAGAACAAGAGAAA
  GAAGAAATTGCTAGCAAAAGTGCATTGCTTCGGCAAATTAAAGAGGTTACTGTGCATAATGATAGTGATG
  ATACTCTTTATGGAAGTTTAACTAACTCTTTGAATATCTCAGAGTTTGAAGAATCCATAAAAGATTATGA
  ACAAGCCAACTTGAATATGGCTAATAGTATAAAATTTTCTGTGTGGGTTTCTTTCTTTGAAATTTACAAT
  GAATATATTTATGACTTATTTGTTCCTGTATCATCTAAATTCCAAAAGAGAAAGATGCTGCGCCTTTCCC
  AAGACGTAAAGGGCTATTCTTTTATAAAAGATCTACAATGGATTCAAGTATCTGATTCCAAAGAAGCCTA
  TAGACTTTTAAAACTAGGAATAAAGCACCAGAGTGTTGCCTTCACAAAATTGAATAATGCTTCCAGTAGA
  AGTCACAGCATATTCACTGTTAAAATATTACAGATTGAAGATTCTGAAATGTCTCGTGTAATTCGAGTCA
  GTGAATTATCTTTATGTGATCTTGCTGGTTCAGAACGAACTATGAAGACACAGAATGAAGGTGAAAGGTT
  AAGAGAGACTGGGAATATCAACACTTCTTTATTGACTCTGGGAAAGTGTATTAACGTCTTGAAGAATAGT
  GAAAAGTCAAAGTTTCAACAGCATGTGCCTTTCCGGGAAAGTAAACTGACTCACTATTTTCAAAGTTTTT
  TTAATGGTAAAGGGAAAATTTGTATGATTGTCAATATCAGCCAATGTTATTTAGCCTATGATGAAACACT
  CAATGTATTGAAGTTCTCCGCCATTGCACAAAAAGTTTGTGTCCCAGACACTTTAAATTCCTCTCAAGAG
  AAATTATTTGGACCTGTCAAATCTTCTCAAGATGTATCACTAGACAGTAATTCAAACAGTAAAATATTAA
  ATGTAAAAAGAGCCACCATTTCATGGGAAAATAGTCTAGAAGATTTGATGGAAGACGAGGATTTGGTTGA
  GGAGCTAGAAAACGCTGAAGAAACTCAAAATGTGGAAACTAAACTTCTTGATGAAGATCTAGATAAAACA
  TTAGAGGAAAATAAGGCTTTCATTAGCCACGAGGAGAAAAGAAAACTGTTGGACTTAATAGAAGACTTGA
  AAAAAAAACTGATAAATGAAAAAAAGGAAAAATTAACCTTGGAATTTAAAATTCGAGAAGAAGTTACACA
  GGAGTTTACTCAGTATTGGGCTCAACGGGAAGCTGACTTTAAGGAGACTCTGCTTCAAGAACGAGAGATA
  TTAGAAGAAAATGCTGAACGTCGTTTGGCTATCTTCAAGGATTTGGTTGGTAAATGTGACACTCGAGAAG
  AAGCAGCGAAAGACATTTGTGCCACAAAAGTTGAAACTGAAGAAACACATAATTATGTAGGATTTGAAGA
  TATTATTGATTCTCTTCAAGATAATGTTGCTGATATTAAGAAACAGGCTGAAATTGCTCACTTATATATT
  GCATCTCTTCCTGACCCCCAGGAAGCTACTGCTTGTTTAGAACTAAAGTTTAATCAAATTAAAGCTGAAT
  TAGCTAAAACCAAAGGAGAATTAATCAAAACCAAAGAAGAGTTAAAAAAGAGAGAAAATGAATCAGATTC
  ATTGATTCAAGAGCTTGAGACATCTAATAAGAAAATAATTACACAGAATCAAAGAATTAAAGAATTGATA
  AATATAATTGATCAAAAAGAAGATACTATCAACGAATTTCAGAACCTAAAGTCTCATATGGAAAACACAT
  TTAAATGCAATGACAAGGCTGATACATCTTCTTTAATAATAAACAATAAATTGATTTGTAATGAAACAGT
  TGAAGTACCTAAGGACAGCAAATCTAAAATCTGTTCAGAAAGAAAAAGAGTAAATGAAAATGAACTTCAG
  CAAGATGAACCACCAGCAAAGAAAGGGTCTATCCATGTTAGTTCAGCTATCACTGAAGACCAAAAGAAAA
  GTGAAGAAGTGCGACCGAACATTGCAGAAATTGAAGACATCAGAGTTTTACAAGAAAATAATGAAGGACT
  GAGAGCATTTTTACTCACTATTGAGAATGAACTTAAAAATGAAAAGGAAGAAAAAGCAGAATTAAATAAA
  CAGATTGTTCATTTTCAGCAGGAACTTTCTCTTTCTGAAAAAAAGAATTTAACTTTAAGTAAAGAGGTCC
  AACAAATTCAGTCAAATTATGATATTGCAATTGCTGAATTACATGTGCAGAAAAGTAAAAATCAAGAACA
  GGAGGAAAAGATCATGAAATTGTCAAATGAGATAGAAACTGCTACAAGAAGCATTACAAATAATGTTTCA
  CAAATAAAATTAATGCACACGAAAATAGACGAACTACGTACTCTTGATTCAGTTTCTCAGATTTCAAACA
  TAGATTTGCTCAATCTCAGGGATCTGTCAAATGGTTCTGAGGAGGATAATTTGCCAAATACACAGTTAGA
  CCTTTTAGGTAATGATTATTTGGTAAGTAAGCAAGTTAAAGAATATCGAATTCAAGAACCCAATAGGGAA
  AATTCTTTCCACTCTAGTATTGAAGCTATTTGGGAAGAATGTAAAGAGATTGTGAAGGCCTCTTCCAAAA
  AAAGTCATCAGATTGAGGAACTGGAACAACAAATTGAAAAATTGCAGGCAGAAGTAAAAGGCTATAAGGA
  TGAAAACAATAGACTAAAGGAGAAGGAGCATAAAAACCAAGATGACCTACTAAAAGAAAAAGAAACTCTT
  ATACAGCAGCTGAAAGAAGAATTGCAAGAAAAAAATGTTACTCTTGATGTTCAAATACAGCATGTAGTTG
  AAGGAAAGAGAGCGCTTTCAGAACTTACACAAGGTGTTACTTGCTATAAGGCAAAAATAAAGGAACTTGA
  AACAATTTTAGAGACTCAGAAAGTTGAATGTAGTCATTCAGCCAAGTTAGAACAAGACATTTTGGAAAAG
  GAATCTATCATCTTAAAGCTAGAAAGAAATTTGAAGGAATTTCAAGAACATCTTCAGGATTCTGTCAAAA
  ACACCAAAGATTTAAATGTAAAGGAACTCAAGCTGAAAGAAGAAATCACACAGTTAACAAATAATTTGCA
  AGATATGAAACATTTACTTCAATTAAAAGAAGAAGAAGAAGAAACCAACAGGCAAGAAACAGAAAAATTG
  AAAGAGGAACTCTCTGCAAGCTCTGCTCGTACCCAGAATCTGAAAGCAGATCTTCAGAGGAAGGAAGAAG
  ATTATGCTGACCTGAAAGAGAAACTGACTGATGCCAAAAAGCAGATTAAGCAAGTACAGAAAGAGGTATC
  TGTAATGCGTGATGAGGATAAATTACTGAGGATTAAAATTAATGAACTGGAGAAAAAGAAAAACCAGTGT
  TCTCAGGAATTAGATATGAAACAGCGAACCATTCAGCAACTCAAGGAGCAGTTAAATAATCAGAAAGTGG
  AAGAAGCTATACAACAGTATGAGAGAGCATGCAAAGATCTAAATGTTAAAGAGAAAATAATTGAAGACAT
  GCGAATGACACTAGAAGAACAGGAACAAACTCAGGTAGAACAGGATCAAGTGCTTGAGGCTAAATTAGAG
  GAAGTTGAAAGGCTGGCCACAGAATTGGAAAAATGGAAGGAAAAATGCAATGATTTGGAAACCAAAAACA
  ATCAAAGGTCAAATAAAGAACATGAGAACAACACAGATGTGCTTGGAAAGCTCACTAATCTTCAAGATGA
  GTTACAGGAGTCTGAACAGAAATATAATGCTGATAGAAAGAAATGGTTAGAAGAAAAAATGATGCTTATC
  ACTCAAGCGAAAGAAGCAGAGAATATACGAAATAAAGAGATGAAAAAATATGCTGAGGACAGGGAGCGTT
  TTTTTAAGCAACAGAATGAAATGGAAATACTGACAGCCCAGCTGACAGAGAAAGATAGTGACCTTCAAAA
  GTGGCGAGAAGAACGAGATCAACTGGTTGCAGCTTTAGAAATACAGCTAAAAGCACTGATATCCAGTAAT
  GTACAGAAAGATAATGAAATTGAACAACTAAAAAGGATCATATCAGAGACTTCTAAAATAGAAACACAAA
  TCATGGATATCAAGCCCAAACGTATTAGTTCAGCAGATCCTGACAAACTTCAAACTGAACCTCTATCGAC
  AAGTTTTGAAATTTCCAGAAATAAAATAGAGGATGGATCTGTAGTCCTTGACTCTTGTGAAGTGTCAACA
  GAAAATGATCAAAGCACTCGATTTCCAAAACCTGAGTTAGAGATTCAATTTACACCTTTACAGCCAAACA
  AAATGGCAGTGAAACACCCTGGTTGTACCACACCAGTGACAGTTAAGATTCCCAAGGCTCGGAAGAGGAA
  GAGTAATGAAATGGAGGAGGACTTGGTGAAATGTGAAAATAAGAAGAATGCTACACCCAGAACTAATTTG
  AAATTTCCTATTTCAGATGATAGAAATTCTTCTGTCAAAAAGGAACAAAAGGTTGCCATACGTCCATCAT
  CTAAGAAAACATATTCTTTACGGAGTCAGGCATCCATAATTGGTGTAAACCTGGCCACTAAGAAAAAAGA
  AGGAACACTACAGAAATTTGGAGACTTCTTACAACATTCTCCCTCAATTCTTCAATCAAAAGCAAAGAAG
  ATAATTGAAACAATGAGCTCTTCAAAGCTCTCAAATGTAGAAGCAAGTAAAGAAAATGTGTCTCAACCAA
  AACGAGCCAAACGGAAATTATACACAAGTGAAATTTCATCTCCTATTGATATATCAGGCCAAGTGATTTT
  AATGGACCAGAAAATGAAGGAGAGTGATCACCAGATTATCAAACGACGACTTCGAACAAAAACAGCCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC240214 representing NM_001284259
  Red=Cloning site Green=Tags(s)
  
  MESNFNQEGVPRPSYVFSADPIARPSEINFDGIKLDLSHEFSLVAPNTEANSFESKDYLQVCLRIRPFTQ
  SEKELESEGCVHILDSQTVVLKEPQCILGRLSEKSSGQMAQKFSFSKVFGPATTQKEFFQGCIMQPVKDL
  LKGQSRLIFTYGLTNSGKTYTFQGTEENIGILPRTLNVLFDSLQERLYTKMNLKPHRSREYLRLSSEQEK
  EEIASKSALLRQIKEVTVHNDSDDTLYGSLTNSLNISEFEESIKDYEQANLNMANSIKFSVWVSFFEIYN
  EYIYDLFVPVSSKFQKRKMLRLSQDVKGYSFIKDLQWIQVSDSKEAYRLLKLGIKHQSVAFTKLNNASSR
  SHSIFTVKILQIEDSEMSRVIRVSELSLCDLAGSERTMKTQNEGERLRETGNINTSLLTLGKCINVLKNS
  EKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCYLAYDETLNVLKFSAIAQKVCVPDTLNSSQE
  KLFGPVKSSQDVSLDSNSNSKILNVKRATISWENSLEDLMEDEDLVEELENAEETQNVETKLLDEDLDKT
  LEENKAFISHEEKRKLLDLIEDLKKKLINEKKEKLTLEFKIREEVTQEFTQYWAQREADFKETLLQEREI
  LEENAERRLAIFKDLVGKCDTREEAAKDICATKVETEETHNYVGFEDIIDSLQDNVADIKKQAEIAHLYI
  ASLPDPQEATACLELKFNQIKAELAKTKGELIKTKEELKKRENESDSLIQELETSNKKIITQNQRIKELI
  NIIDQKEDTINEFQNLKSHMENTFKCNDKADTSSLIINNKLICNETVEVPKDSKSKICSERKRVNENELQ
  QDEPPAKKGSIHVSSAITEDQKKSEEVRPNIAEIEDIRVLQENNEGLRAFLLTIENELKNEKEEKAELNK
  QIVHFQQELSLSEKKNLTLSKEVQQIQSNYDIAIAELHVQKSKNQEQEEKIMKLSNEIETATRSITNNVS
  QIKLMHTKIDELRTLDSVSQISNIDLLNLRDLSNGSEEDNLPNTQLDLLGNDYLVSKQVKEYRIQEPNRE
  NSFHSSIEAIWEECKEIVKASSKKSHQIEELEQQIEKLQAEVKGYKDENNRLKEKEHKNQDDLLKEKETL
  IQQLKEELQEKNVTLDVQIQHVVEGKRALSELTQGVTCYKAKIKELETILETQKVECSHSAKLEQDILEK
  ESIILKLERNLKEFQEHLQDSVKNTKDLNVKELKLKEEITQLTNNLQDMKHLLQLKEEEEETNRQETEKL
  KEELSASSARTQNLKADLQRKEEDYADLKEKLTDAKKQIKQVQKEVSVMRDEDKLLRIKINELEKKKNQC
  SQELDMKQRTIQQLKEQLNNQKVEEAIQQYERACKDLNVKEKIIEDMRMTLEEQEQTQVEQDQVLEAKLE
  EVERLATELEKWKEKCNDLETKNNQRSNKEHENNTDVLGKLTNLQDELQESEQKYNADRKKWLEEKMMLI
  TQAKEAENIRNKEMKKYAEDRERFFKQQNEMEILTAQLTEKDSDLQKWREERDQLVAALEIQLKALISSN
  VQKDNEIEQLKRIISETSKIETQIMDIKPKRISSADPDKLQTEPLSTSFEISRNKIEDGSVVLDSCEVST
  ENDQSTRFPKPELEIQFTPLQPNKMAVKHPGCTTPVTVKIPKARKRKSNEMEEDLVKCENKKNATPRTNL
  KFPISDDRNSSVKKEQKVAIRPSSKKTYSLRSQASIIGVNLATKKKEGTLQKFGDFLQHSPSILQSKAKK
  IIETMSSSKLSNVEASKENVSQPKRAKRKLYTSEISSPIDISGQVILMDQKMKESDHQIIKRRLRTKTAK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001284259
• ORF Size: 5460 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001284259.2
• RefSeq Size: 6459 bp
• RefSeq ORF: 5463 bp
• Locus ID: 9585
• UniProt ID: Q96Q89
• Cytogenetics: 10q23.31
• Protein Families: Druggable Genome
• MW: 211.1 kDa
• Gene Summary: Plus-end-directed motor enzyme that is required for completion of cytokinesis (PubMed:11470801, PubMed:12740395). Required for proper midbody organization and abscission in polarized cortical stem cells. Plays a role in the regulation of neuronal polarization by mediating the transport of specific cargos. Participates in the mobilization of SHTN1 and in the accumulation of PIP3 in the growth cone of primary hippocampal neurons in a tubulin and actin-dependent manner. In the developing telencephalon, cooperates with SHTN1 to promote both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex. Involved in cerebral cortex growth (By similarity). Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression (PubMed:17409436).[UniProtKB/Swiss-Prot Function]