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TET3 (NM_001287491) Human Tagged ORF Clone
CAT#: RC240211
- TrueORF®
TET3 (myc-DDK-tagged) - Human tet methylcytosine dioxygenase 3 (TET3)
ORF Plasmid: tGFP
"NM_001287491" in other vectors (2)
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Other products for "TET3"
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | TET3 |
| Synonyms | BEFAHRS; hCG_40738 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC240211 representing NM_001287491
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCAGTTTCAGGTGCCCCTGGCCGTCCAGCCGGACCTGCCAGGCCTTTATGACTTCCCTCAGCGCC AGGTGATGGTAGGGAGCTTCCCGGGGTCTGGGCTCTCCATGGCTGGGAGTGAGTCCCAACTCCGAGGGGG TGGAGATGGTCGAAAGAAACGGAAACGGTGTGGTACTTGTGAGCCCTGCCGGCGGCTGGAAAACTGTGGC GCTTGCACTAGCTGTACCAACCGCCGCACGCACCAGATCTGCAAACTGCGAAAATGTGAGGTGCTGAAGA AAAAAGTAGGGCTTCTCAAGGAGGTGGAAATAAAGGCTGGTGAAGGAGCCGGGCCGTGGGGACAAGGAGC GGCTGTCAAGACAGGCTCAGAGCTCAGCCCAGTTGATGGACCTGTTCCAGGTCAGATGGACTCAGGGCCA GTGTACCATGGGGACTCACGGCAGCTAAGCGCCTCAGGGGTGCCGGTCAATGGTGCTAGAGAGCCCGCTG GACCCAGTCTGCTGGGGACTGGGGGTCCTTGGCGGGTAGACCAAAAGCCCGACTGGGAGGCTGCCCCAGG CCCAGCTCATACTGCTCGCCTGGAAGATGCCCACGATCTGGTGGCCTTTTCGGCTGTGGCCGAAGCTGTG TCCTCTTATGGGGCCCTTAGCACCCGGCTCTATGAAACCTTCAACCGTGAGATGAGTCGTGAGGCTGGGA ACAACAGCAGGGGACCCCGGCCAGGGCCTGAGGGCTGCTCTGCTGGCAGCGAAGACCTTGACACACTGCA GACGGCCCTGGCCCTCGCGCGGCATGGTATGAAACCACCCAACTGCAACTGCGATGGCCCAGAATGCCCT GACTACCTCGAGTGGCTGGAGGGGAAGATCAAGTCTGTGGTCATGGAAGGAGGGGAGGAGCGGCCCAGGC TCCCAGGGCCTCTGCCTCCTGGTGAGGCCGGCCTCCCAGCACCAAGCACCAGGCCACTCCTCAGCTCAGA GGTGCCCCAGATCTCTCCCCAAGAGGGCCTGCCCCTGTCCCAGAGTGCCCTGAGCATTGCCAAGGAAAAA AACATCAGCTTGCAGACCGCCATTGCCATTGAGGCCCTCACACAGCTCTCCTCTGCCCTCCCGCAGCCTT CTCATTCCACCCCCCAGGCTTCTTGCCCCCTTCCTGAGGCCTTGTCACCTCCTGCCCCTTTCAGATCTCC CCAGTCTTACCTCCGGGCTCCCTCATGGCCTGTGGTTCCTCCTGAAGAGCACTCATCTTTTGCTCCTGAT AGCTCTGCCTTCCCTCCAGCAACTCCTAGAACTGAGTTCCCTGAAGCCTGGGGCACTGACACCCCTCCAG CAACGCCCCGGAGCTCCTGGCCCATGCCTCGCCCAAGCCCCGATCCCATGGCTGAACTGGAGCAGTTGTT GGGCAGCGCCAGTGATTACATCCAGTCAGTATTCAAGCGGCCTGAGGCCCTGCCTACCAAGCCCAAGGTC AAGGTGGAGGCACCCTCTTCCTCCCCGGCCCCGGCCCCATCCCCTGTACTTCAGAGGGAGGCTCCCACGC CATCCTCGGAGCCCGACACCCACCAGAAGGCCCAGACCGCCCTGCAGCAGCACCTCCACCACAAGCGCAG CCTCTTCCTAGAACAGGTGCACGACACCTCCTTCCCTGCTCCTTCAGAGCCTTCTGCTCCTGGCTGGTGG CCCCCACCAAGTTCACCTGTCCCACGGCTTCCAGACAGACCACCCAAGGAGAAGAAGAAGAAGCTCCCAA CACCAGCTGGAGGTCCCGTGGGAACGGAGAAAGCTGCCCCTGGGATCAAGCCCAGTGTCCGAAAGCCCAT TCAGATCAAGAAGTCCAGGCCCCGGGAAGCACAGCCCCTCTTCCCACCTGTCCGACAGATTGTCCTGGAA GGGCTTAGGTCCCCAGCCTCCCAGGAAGTGCAGGCTCATCCACCGGCCCCTCTGCCTGCCTCACAGGGCT CTGCTGTGCCCCTGCCCCCAGAACCTTCTCTTGCGCTATTTGCACCTAGTCCCTCCAGGGACAGCCTGCT GCCCCCTACTCAGGAAATGAGGTCCCCCAGCCCCATGACAGCCTTGCAGCCAGGCTCCACTGGCCCTCTT CCCCCTGCCGATGACAAGCTGGAAGAGCTCATCCGGCAGTTTGAGGCTGAATTTGGAGATAGCTTTGGGC TTCCCGGCCCCCCTTCTGTGCCCATTCAGGACCCCGAGAACCAGCAAACATGTCTCCCAGCCCCTGAGAG CCCCTTTGCTACCCGTTCCCCCAAGCAAATCAAGATTGAGTCTTCGGGGGCTGTGACTGTGCTCTCAACC ACCTGCTTCCATTCAGAGGAGGGAGGACAGGAGGCCACACCCACCAAGGCTGAGAACCCACTCACACCCA CCCTCAGTGGCTTCTTGGAGTCACCTCTTAAGTACCTGGACACACCCACCAAGAGTCTGCTGGACACACC TGCCAAGAGAGCCCAGGCCGAGTTCCCCACCTGCGATTGCGTCGAACAAATAGTGGAGAAAGATGAAGGT CCATATTATACTCACTTGGGATCTGGCCCCACGGTCGCCTCTATCCGGGAACTCATGGAGGAGCGGTATG GAGAGAAGGGGAAAGCCATCCGGATCGAGAAGGTCATCTACACGGGGAAGGAGGGAAAGAGCTCCCGCGG TTGCCCCATTGCAAAGTGGGTGATCCGCAGGCACACGCTGGAGGAGAAGCTACTCTGCCTGGTGCGGCAC CGGGCAGGCCACCACTGCCAGAACGCTGTGATCGTCATCCTCATCCTGGCCTGGGAGGGCATTCCCCGTA GCCTCGGAGACACCCTCTACCAGGAGCTCACCGACACCCTCCGGAAGTATGGGAACCCCACCAGCCGGAG ATGCGGCCTCAACGATGACCGGACCTGCGCTTGCCAAGGCAAAGACCCCAACACCTGTGGTGCCTCCTTC TCCTTTGGTTGTTCCTGGAGCATGTACTTCAACGGCTGCAAGTATGCTCGGAGCAAGACACCTCGCAAGT TCCGCCTCGCAGGGGACAATCCCAAAGAGGAAGAAGTGCTCCGGAAGAGTTTCCAGGACCTGGCCACCGA AGTCGCTCCCCTGTACAAGCGACTGGCCCCTCAGGCCTATCAGAACCAGGTGACCAACGAGGAAATAGCG ATTGACTGCCGTCTGGGGCTGAAGGAAGGACGGCCCTTCGCGGGGGTCACGGCCTGCATGGACTTCTGTG CCCACGCCCACAAGGACCAGCATAACCTCTACAATGGGTGCACCGTGGTCTGCACCCTGACCAAGGAAGA CAATCGCTGCGTGGGCAAGATTCCCGAGGATGAGCAGCTGCATGTTCTCCCCCTGTACAAGATGGCCAAC ACGGATGAGTTTGGTAGCGAGGAGAACCAGAATGCAAAGGTGGGCAGCGGAGCCATCCAGGTGCTCACCG CCTTCCCCCGCGAGGTCCGACGCCTGCCCGAGCCTGCCAAGTCCTGCCGCCAGCGGCAGCTGGAAGCCAG AAAGGCAGCAGCCGAGAAGAAGAAGATTCAGAAGGAGAAGCTGAGCACTCCGGAGAAGATCAAGCAGGAG GCCCTGGAGCTGGCGGGCATTACGTCGGACCCAGGCCTGTCTCTGAAGGGTGGATTGTCCCAGCAAGGCC TGAAGCCCTCCCTCAAGGTGGAGCCGCAGAACCACTTCAGCTCCTTCAAGTACAGCGGCAACGCGGTGGT GGAGAGCTACTCGGTGCTGGGCAACTGCCGGCCCTCCGACCCTTACAGCATGAACAGCGTGTACTCCTAC CACTCCTACTATGCACAGCCCAGCCTGACCTCCGTCAATGGCTTCCACTCCAAGTACGCTCTCCCGTCTT TTAGCTACTATGGCTTTCCATCCAGCAACCCCGTCTTCCCCTCTCAGTTCCTGGGTCCTGGTGCCTGGGG GCACAGTGGCAGCAGTGGCAGTTTTGAGAAGAAGCCAGACCTCCACGCTCTGCACAACAGCCTGAGCCCG GCCTACGGTGGTGCTGAGTTTGCCGAGCTGCCCAGCCAGGCTGTTCCCACAGACGCCCACCACCCCACTC CTCACCACCAGCAGCCTGCGTACCCAGGCCCCAAGGAGTATCTGCTTCCCAAGGCCCCCCTACTCCACTC AGTGTCCAGGGACCCCTCCCCCTTTGCCCAGAGCTCCAACTGCTACAACAGATCCATCAAGCAAGAGCCA GTAGACCCGCTGACCCAGGCTGAGCCTGTGCCCAGAGACGCTGGCAAGATGGGCAAGACACCTCTGTCCG AGGTGTCTCAGAATGGAGGACCCAGTCACCTTTGGGGACAGTACTCAGGAGGCCCAAGCATGTCCCCCAA GAGGACTAACGGTGTGGGTGGCAGCTGGGGTGTGTTCTCGTCTGGGGAGAGTCCTGCCATCGTCCCTGAC AAGCTCAGTTCCTTTGGGGCCAGCTGCCTGGCCCCTTCCCACTTCACAGATGGCCAGTGGGGGCTGTTCC CCGGTGAGGGGCAGCAGGCAGCTTCCCACTCTGGAGGACGGCTGCGAGGCAAACCGTGGAGCCCCTGCAA GTTTGGGAACAGCACCTCGGCCTTGGCTGGGCCCAGCCTGACTGAGAAGCCGTGGGCGCTGGGGGCAGGG GATTTCAACTCGGCCCTGAAAGGTAGTCCTGGGTTCCAAGACAAGCTGTGGAACCCCATGAAAGGAGAGG AGGGCAGGATTCCAGCCGCAGGGGCCAGCCAGCTGGACAGGGCCTGGCAGTCCTTTGGTCTGCCCCTGGG ATCCAGCGAGAAGCTGTTTGGGGCTCTGAAGTCAGAGGAGAAGCTGTGGGACCCCTTCAGCCTGGAGGAG GGGCCGGCTGAGGAGCCCCCCAGCAAGGGAGCGGTGAAGGAGGAGAAGGGCGGTGGTGGTGCGGAGGAGG AAGAGGAGGAGCTGTGGTCGGACAGTGAACACAACTTCCTGGACGAGAACATCGGCGGCGTGGCCGTGGC CCCAGCCCACGGCTCCATCCTCATCGAGTGTGCCCGGCGGGAGCTGCACGCCACCACGCCGCTTAAGAAG CCCAACCGCTGCCACCCCACCCGCATCTCGCTGGTCTTCTACCAGCACAAGAACCTCAACCAGCCCAACC ACGGGCTGGCCCTCTGGGAAGCCAAGATGAAGCAGCTGGCGGAGAGGGCACGGGCACGGCAGGAGGAGGC TGCCCGGCTGGGCCTGGGCCAGCAGGAGGCCAAGCTCTACGGGAAGAAGCGCAAGTGGGGGGGCACTGTG GTTGCTGAGCCCCAGCAGAAAGAGAAGAAGGGGGTCGTCCCCACCCGGCAGGCACTGGCTGTGCCCACAG ACTCGGCGGTCACCGTGTCCTCCTATGCCTACACGAAGGTCACTGGCCCCTACAGCCGCTGGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC240211 representing NM_001287491
Red=Cloning site Green=Tags(s) MSQFQVPLAVQPDLPGLYDFPQRQVMVGSFPGSGLSMAGSESQLRGGGDGRKKRKRCGTCEPCRRLENCG ACTSCTNRRTHQICKLRKCEVLKKKVGLLKEVEIKAGEGAGPWGQGAAVKTGSELSPVDGPVPGQMDSGP VYHGDSRQLSASGVPVNGAREPAGPSLLGTGGPWRVDQKPDWEAAPGPAHTARLEDAHDLVAFSAVAEAV SSYGALSTRLYETFNREMSREAGNNSRGPRPGPEGCSAGSEDLDTLQTALALARHGMKPPNCNCDGPECP DYLEWLEGKIKSVVMEGGEERPRLPGPLPPGEAGLPAPSTRPLLSSEVPQISPQEGLPLSQSALSIAKEK NISLQTAIAIEALTQLSSALPQPSHSTPQASCPLPEALSPPAPFRSPQSYLRAPSWPVVPPEEHSSFAPD SSAFPPATPRTEFPEAWGTDTPPATPRSSWPMPRPSPDPMAELEQLLGSASDYIQSVFKRPEALPTKPKV KVEAPSSSPAPAPSPVLQREAPTPSSEPDTHQKAQTALQQHLHHKRSLFLEQVHDTSFPAPSEPSAPGWW PPPSSPVPRLPDRPPKEKKKKLPTPAGGPVGTEKAAPGIKPSVRKPIQIKKSRPREAQPLFPPVRQIVLE GLRSPASQEVQAHPPAPLPASQGSAVPLPPEPSLALFAPSPSRDSLLPPTQEMRSPSPMTALQPGSTGPL PPADDKLEELIRQFEAEFGDSFGLPGPPSVPIQDPENQQTCLPAPESPFATRSPKQIKIESSGAVTVLST TCFHSEEGGQEATPTKAENPLTPTLSGFLESPLKYLDTPTKSLLDTPAKRAQAEFPTCDCVEQIVEKDEG PYYTHLGSGPTVASIRELMEERYGEKGKAIRIEKVIYTGKEGKSSRGCPIAKWVIRRHTLEEKLLCLVRH RAGHHCQNAVIVILILAWEGIPRSLGDTLYQELTDTLRKYGNPTSRRCGLNDDRTCACQGKDPNTCGASF SFGCSWSMYFNGCKYARSKTPRKFRLAGDNPKEEEVLRKSFQDLATEVAPLYKRLAPQAYQNQVTNEEIA IDCRLGLKEGRPFAGVTACMDFCAHAHKDQHNLYNGCTVVCTLTKEDNRCVGKIPEDEQLHVLPLYKMAN TDEFGSEENQNAKVGSGAIQVLTAFPREVRRLPEPAKSCRQRQLEARKAAAEKKKIQKEKLSTPEKIKQE ALELAGITSDPGLSLKGGLSQQGLKPSLKVEPQNHFSSFKYSGNAVVESYSVLGNCRPSDPYSMNSVYSY HSYYAQPSLTSVNGFHSKYALPSFSYYGFPSSNPVFPSQFLGPGAWGHSGSSGSFEKKPDLHALHNSLSP AYGGAEFAELPSQAVPTDAHHPTPHHQQPAYPGPKEYLLPKAPLLHSVSRDPSPFAQSSNCYNRSIKQEP VDPLTQAEPVPRDAGKMGKTPLSEVSQNGGPSHLWGQYSGGPSMSPKRTNGVGGSWGVFSSGESPAIVPD KLSSFGASCLAPSHFTDGQWGLFPGEGQQAASHSGGRLRGKPWSPCKFGNSTSALAGPSLTEKPWALGAG DFNSALKGSPGFQDKLWNPMKGEEGRIPAAGASQLDRAWQSFGLPLGSSEKLFGALKSEEKLWDPFSLEE GPAEEPPSKGAVKEEKGGGGAEEEEEELWSDSEHNFLDENIGGVAVAPAHGSILIECARRELHATTPLKK PNRCHPTRISLVFYQHKNLNQPNHGLALWEAKMKQLAERARARQEEAARLGLGQQEAKLYGKKRKWGGTV VAEPQQKEKKGVVPTRQALAVPTDSAVTVSSYAYTKVTGPYSRWI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001287491 |
| ORF Size | 5385 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001287491.2 |
| RefSeq Size | 11405 bp |
| RefSeq ORF | 5388 bp |
| Locus ID | 200424 |
| UniProt ID | O43151 |
| Cytogenetics | 2p13.1 |
| Protein Families | Transcription Factors |
| MW | 194.2 kDa |
| Gene Summary | Members of the ten-eleven translocation (TET) gene family, including TET3, play a role in the DNA methylation process (Langemeijer et al., 2009 [PubMed 19923888]).[supplied by OMIM, Nov 2010] |
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