NUP153 (NM_001278210) Human Tagged ORF Clone

CAT#: RC240142

NUP153 (myc-DDK-tagged) - Human nucleoporin 153kDa (NUP153), transcript variant 3

Product Images

Circular map for RC240142

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NUP153
• Synonyms: HNUP153; N153
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC240142 representing NM_001278210
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCGGGAGCCGGAGGAGTCGGAGGGGGCGGTGGCGGCAAGATCCGGACGCGGCGTTGCCACCAGG
  GGCCAATTAAGCCTTACCAGCAGGGGCGACAACAGCATCAGGGCATTCTTAGCAGGGTTACAGAATCTGT
  TAAGAATATTGTGCCAGGGTGGCTACAAAGATACTTCAACAAGAATGAAGATGTATGCAGCTGTTCAACA
  GACACAAGCGAGGTTCCACGCTGGCCAGAAAATAAAGAGGACCATCTGGTATATGCCGATGAGGAGAGCT
  CTAATATTACTGATGGGAGAATCACACCTGAGCCAGCAGTCAGTAATACAGAAGAACCTTCAACAACTAG
  TACTGCTTCAAATTATCCAGATGTGTTAACAAGGCCTTCTCTTCATCGGAGCCATCTGAATTTTTCCATG
  TTGGAATCCCCTGCATTACACTGTCAGCCATCTACATCCTCGGCATTCCCAATTGGCAGTTCGGGATTTT
  CCCTTGTAAAGGAAATTAAAGATTCTACCTCTCAGCATGATGATGATAACATCTCAACTACCAGTGGTTT
  TTCTTCAAGAGCTTCTGATAAAGATATAACTGTTTCAAAGAACACTTCATTGCCACCTCTGTGGTCCCCA
  GAAGCTGAACGTTCTCACTCACTCTCACAGCACACTGCCACCAGCTCAAAAAAACCAGCATTCAACTTGT
  CTGCCTTTGGAACACTTTCCCCTTCACTTGGGAATTCTTCAATCCTTAAAACCAGTCAGCTTGGAGATTC
  TCCTTTTTATCCTGGAAAAACAACATACGGTGGGGCAGCAGCTGCTGTAAGACAGTCTAAACTACGAAAT
  ACACCTTATCAGGCACCAGTTAGAAGACAAATGAAAGCTAAGCAACTCAGTGCACAATCTTACGGTGTGA
  CCAGTTCAACAGCTCGGCGAATATTGCAGTCTTTAGAGAAGATGTCAAGCCCTTTAGCGGATGCAAAAAG
  AATTCCATCCATTGTTTCTTCTCCTCTGAATTCTCCTCTTGATAGGAGTGGGATAGATATCACAGATTTT
  CAGGCCAAAAGAGAAAAGGTGGATTCTCAATATCCTCCTGTTCAGAGACTTATGACCCCAAAGCCAGTTT
  CCATAGCAACAAATCGAAGTGTTTATTTTAAACCATCTCTGACTCCTTCTGGTGAATTCAGGAAGACTAA
  TCAAAGAATAGATAACAAGTGCAGTACTGGATATGAAAAAAATATGACACCCGGACAAAATAGAGAACAA
  CGAGAAAGTGGCTTTTCATATCCAAATTTCAGTTTGCCTGCAGCCAATGGTTTATCTTCTGGAGTAGGTG
  GTGGAGGTGGCAAGATGAGACGAGAAAGAACACGCTTTGTTGCTTCTAAACCTCTGGAGGAGGAGGAAAT
  GGAAGTTCCAGTATTACCGAAAATCTCTCTACCGATCACCAGTTCTTCACTGCCTACCTTTAATTTTAGT
  TCCCCTGAGATCACAACTTCCTCTCCATCACCCATCAATTCGTCTCAAGCATTAACAAACAAGGTACAAA
  TGACCTCTCCGAGCAGCACTGGCAGTCCCATGTTTAAATTTTCATCTCCAATCGTAAAATCTACTGAGGC
  AAATGTACTACCTCCATCATCTATTGGATTTACATTTAGTGTGCCTGTTGCAAAAACAGCAGAACTTTCT
  GGTTCTAGTAGTACTTTAGAACCAATTATAAGTAGTTCAGGTTTCGCATCGCCGAAGATAGATTCTGTTG
  CTGCTCAGCCCACCGCAACAAGCCCAGTAGTTTATACAAGACCAGCAATAAGTAGCTTTTCTTCTAGTGG
  AATTGGGTTTGGGGAGAGTTTAAAAGCTGGGTCATCATGGCAGTGTGATACATGTCTACTCCAGAACAAA
  GTTACAGACAACAAATGCATAGCCTGTCAAGCAGCAAAATTGTCACCCAGAGATACTGCTAAACAGACTG
  GAATTGAAACACCAAATAAAAGTGGCAAAACAACTCTTTCTGCATCAGGGACAGGCTTTGGAGACAAATT
  TAAACCAGTGATAGGCACTTGGGATTGTGATACCTGTTTAGTGCAAAATAAACCTGAAGCAATAAAATGT
  GTAGCCTGTGAAACACCGAAACCTGGAACTTGTGTGAAGCGAGCCCTTACATTGACAGTGGTTTCGGAAA
  GTGCTGAGACTATGACTGCTTCATCTTCCAGCTGCACTGTAACCACTGGTACCTTAGGATTTGGAGATAA
  ATTCAAAAGGCCCATTGGATCTTGGGAGTGTTCAGTATGCTGTGTTTCTAATAATGCAGAAGACAATAAG
  TGTGTGTCCTGTATGTCTGAGAAACCAGGAAGTTCAGTACCTGCTTCAAGTAGCAGCACTGTACCTGTCT
  CTCTGCCTTCTGGAGGCTCTCTAGGATTGGAAAAGTTCAAGAAACCCGAGGGAAGCTGGGACTGTGAATT
  GTGCCTAGTGCAGAATAAGGCAGACTCTACCAAATGTTTGGCATGTGAAAGTGCAAAGCCAGGCACAAAA
  TCTGGGTTTAAAGGCTTTGACACATCTTCCTCATCTTCGAACTCAGCAGCCTCCTCATCCTTCAAATTTG
  GTGTCTCATCATCCTCTTCTGGGCCTTCTCAGACTTTAACAAGCACTGGAAATTTTAAATTTGGAGATCA
  GGGAGGATTCAAAATAGGTGTGTCATCCGATTCTGGGTCTATAAACCCCATGAGTGAAGGCTTTAAATTT
  TCTAAACCAATAGGAGATTTTAAATTTGGAGTTTCATCTGAATCTAAGCCCGAAGAAGTTAAAAAAGATA
  GTAAGAATGATAATTTTAAGTTTGGACTTTCTTCTGGTTTAAGCAACCCAGTTTCTTTAACTCCATTTCA
  ATTTGGGGTATCTAATCTTGGACAGGAAGAAAAGAAAGAGGAACTGCCCAAATCTTCCTCTGCAGGTTTT
  AGCTTTGGTACAGGTGTTATTAACTCCACCCCTGCTCCTGCTAACACCATAGTGACCTCTGAGAACAAGA
  GCAGCTTCAACCTTGGAACCATAGAAACCAAGAGTGCTTCAGTGGCTCCTTTCACATGTAAGACATCAGA
  AGCTAAAAAAGAAGAAATGCCTGCCACCAAAGGAGGATTCTCTTTTGGCAACGTGGAGCCTGCCTCTCTG
  CCATCTGCCTCAGTGTTTGTTTTGGGAAGGACAGAAGAGAAACAGCAAGAGCCTGTCACTTCTACTTCCC
  TAGTTTTTGGGAAGAAAGCTGACAATGAAGAGCCAAAGTGTCAACCAGTGTTTTCCTTTGGGAATTCAGA
  GCAAACCAAAGATGAGAATTCTTCAAAGTCCACATTTAGTTTTAGTATGACAAAACCATCTGAGAAGGAA
  TCTGAACAGCCAGCAAAAGCCACTTTTGCCTTTGGAGCTCAAACTAGTACTACAGCTGATCAAGGTGCAG
  CAAAGCCAGTTTTTAGTTTCTTGAACAACAGTTCCTCTAGTTCAAGTACACCAGCCACTTCTGCTGGTGG
  TGGCATATTTGGTAGTTCCACCTCTTCCTCCAATCCACCTGTGGCTACCTTTGTGTTTGGACAGTCCAGC
  AATCCTGTGAGCAGCTCTGCCTTTGGTAACACTGCTGAATCCAGCACCTCTCAGTCTTTGCTATTTTCTC
  AAGATAGCAAACTAGCAACCACATCCAGCACAGGTACAGCTGTCACCCCATTTGTCTTTGGTCCAGGAGC
  CAGCAGTAATAATACTACCACCTCTGGTTTCGGCTTTGGAGCCACAACCACATCTAGCTCTGCAGGATCC
  TCCTTTGTATTTGGAACTGGACCCTCAGCACCATCTGCCAGTCCAGCATTTGGTGCTAACCAGACCCCAA
  CATTTGGACAAAGTCAAGGTGCCAGCCAGCCCAATCCCCCAGGCTTTGGATCTATATCATCTTCCACAGC
  ATTATTTCCCACTGGTTCTCAGCCTGCACCACCTACTTTTGGGACAGTGTCAAGCAGTAGCCAGCCCCCT
  GTGTTTGGACAGCAACCTAGTCAGTCTGCATTTGGCTCTGGAACAACTCCTAATTCTAGTTCGGCTTTCC
  AGTTTGGCAGCAGCACTACAAATTTCAACTTCACAAACAACAGTCCATCAGGAGTGTTCACATTTGGTGC
  AAATTCTAGCACACCTGCAGCCTCAGCCCAGCCTTCAGGCTCGGGGGGCTTTCCATTTAACCAGTCTCCA
  GCAGCATTTACAGTGGGGTCAAATGGGAAAAATGTGTTCTCTTCTTCTGGAACTTCATTCTCTGGTCGCA
  AGATAAAGACTGCTGTTAGACGCAGGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC240142 representing NM_001278210
  Red=Cloning site Green=Tags(s)
  
  MASGAGGVGGGGGGKIRTRRCHQGPIKPYQQGRQQHQGILSRVTESVKNIVPGWLQRYFNKNEDVCSCST
  DTSEVPRWPENKEDHLVYADEESSNITDGRITPEPAVSNTEEPSTTSTASNYPDVLTRPSLHRSHLNFSM
  LESPALHCQPSTSSAFPIGSSGFSLVKEIKDSTSQHDDDNISTTSGFSSRASDKDITVSKNTSLPPLWSP
  EAERSHSLSQHTATSSKKPAFNLSAFGTLSPSLGNSSILKTSQLGDSPFYPGKTTYGGAAAAVRQSKLRN
  TPYQAPVRRQMKAKQLSAQSYGVTSSTARRILQSLEKMSSPLADAKRIPSIVSSPLNSPLDRSGIDITDF
  QAKREKVDSQYPPVQRLMTPKPVSIATNRSVYFKPSLTPSGEFRKTNQRIDNKCSTGYEKNMTPGQNREQ
  RESGFSYPNFSLPAANGLSSGVGGGGGKMRRERTRFVASKPLEEEEMEVPVLPKISLPITSSSLPTFNFS
  SPEITTSSPSPINSSQALTNKVQMTSPSSTGSPMFKFSSPIVKSTEANVLPPSSIGFTFSVPVAKTAELS
  GSSSTLEPIISSSGFASPKIDSVAAQPTATSPVVYTRPAISSFSSSGIGFGESLKAGSSWQCDTCLLQNK
  VTDNKCIACQAAKLSPRDTAKQTGIETPNKSGKTTLSASGTGFGDKFKPVIGTWDCDTCLVQNKPEAIKC
  VACETPKPGTCVKRALTLTVVSESAETMTASSSSCTVTTGTLGFGDKFKRPIGSWECSVCCVSNNAEDNK
  CVSCMSEKPGSSVPASSSSTVPVSLPSGGSLGLEKFKKPEGSWDCELCLVQNKADSTKCLACESAKPGTK
  SGFKGFDTSSSSSNSAASSSFKFGVSSSSSGPSQTLTSTGNFKFGDQGGFKIGVSSDSGSINPMSEGFKF
  SKPIGDFKFGVSSESKPEEVKKDSKNDNFKFGLSSGLSNPVSLTPFQFGVSNLGQEEKKEELPKSSSAGF
  SFGTGVINSTPAPANTIVTSENKSSFNLGTIETKSASVAPFTCKTSEAKKEEMPATKGGFSFGNVEPASL
  PSASVFVLGRTEEKQQEPVTSTSLVFGKKADNEEPKCQPVFSFGNSEQTKDENSSKSTFSFSMTKPSEKE
  SEQPAKATFAFGAQTSTTADQGAAKPVFSFLNNSSSSSSTPATSAGGGIFGSSTSSSNPPVATFVFGQSS
  NPVSSSAFGNTAESSTSQSLLFSQDSKLATTSSTGTAVTPFVFGPGASSNNTTTSGFGFGATTTSSSAGS
  SFVFGTGPSAPSASPAFGANQTPTFGQSQGASQPNPPGFGSISSSTALFPTGSQPAPPTFGTVSSSSQPP
  VFGQQPSQSAFGSGTTPNSSSAFQFGSSTTNFNFTNNSPSGVFTFGANSSTPAASAQPSGSGGFPFNQSP
  AAFTVGSNGKNVFSSSGTSFSGRKIKTAVRRRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278210
• ORF Size: 4299 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278210.1, NP_001265139.1
• RefSeq Size: 5811 bp
• RefSeq ORF: 4302 bp
• Locus ID: 9972
• UniProt ID: P49790
• Cytogenetics: 6p22.3
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• MW: 149.8 kDa
• Gene Summary: Nuclear pore complexes regulate the transport of macromolecules between the nucleus and cytoplasm. They are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are glycoproteins found in nuclear pores and contain characteristic pentapeptide XFXFG repeats as well as O-linked N-acetylglucosamine residues oriented towards the cytoplasm. The protein encoded by this gene has three distinct domains: a N-terminal region containing a pore targeting and an RNA-binding domain domain, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]