CYFIP2 (NM_001291722) Human Tagged ORF Clone
CAT#: RC240089
- TrueORF®
CYFIP2 (myc-DDK-tagged) - Human cytoplasmic FMR1 interacting protein 2 (CYFIP2), transcript variant 5
ORF Plasmid: tGFP
"NM_001291722" in other vectors (2)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | CYFIP2 |
Synonyms | EIEE65; PIR121 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC240089 representing NM_001291722
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCACGCACGTCACCCTGGAAGATGCCCTGTCCAACGTGGACCTGCTTGAAGAGCTTCCCCTCCCCG ACCAGCAGCCATGCATCGAGCCTCCACCTTCCTCCATCATGTACCAGGCTAACTTTGACACAAACTTTGA GGACAGGAATGCATTTGTCACGGGCATTGCAAGGTACATTGAGCAGGCTACAGTCCACTCCAGCATGAAT GAGATGCTGGAGGAAGGACATGAGTATGCGGTCATGCTGTACACCTGGCGCAGCTGTTCCCGGGCCATTC CCCAGGTGAAATGCAACGAGCAGCCCAACCGAGTAGAGATCTATGAGAAGACAGTAGAGGTGCTGGAGCC GGAGGTCACCAAGCTCATGAAGTTCATGTATTTTCAGCGCAAGGCCATCGAGCGGTTCTGCAGCGAGGTG AAGCGGCTGTGCCATGCCGAGCGCAGGAAGGACTTTGTCTCTGAGGCCTACCTCCTGACCCTTGGCAAGT TCATCAACATGTTTGCTGTCCTGGATGAGCTAAAGAACATGAAGTGCAGCGTCAAGAATGACCACTCTGC CTACAAGAGGGCAGCACAGTTCCTGCGGAAGATGGCAGATCCCCAGTCTATCCAGGAGTCGCAGAACCTT TCCATGTTCCTGGCCAACCACAACAGGATCACCCAGTGTCTCCACCAGCAACTTGAAGTGATCCCAGGCT ATGAGGAGCTGCTGGCTGACATTGTCAACATCTGTGTGGATTACTACGAGAACAAGATGTACCTGACTCC CAGTGAGAAACATATGCTCCTCAAGGTGATGGGCTTTGGCCTCTACCTAATGGATGGAAATGTCAGTAAC ATTTACAAACTGGATGCCAAGAAGAGAATTAATCTTAGCAAAATTGATAAATTCTTTAAGCAGCTGCAGG TGGTGCCCCTTTTCGGCGACATGCAGATAGAGCTGGCCAGATACATTAAGACCAGTGCTCACTATGAAGA GAACAAGTCCAAGTGGACGTGCACCCAGAGCAGCATCAGCCCCCAGTACAATATCTGCGAGCAGATGGTT CAGATCCGGGATGACCACATCCGCTTCATCTCCGAGCTCGCTCGCTACAGCAACAGTGAGGTGGTGACGG GCTCAGGGCTGGACAGCCAGAAGTCAGACGAGGAGTATCGCGAGCTCTTCGACCTAGCCCTGCGGGGTCT GCAGCTTCTATCCAAGTGGAGCGCCCACGTCATGGAGGTGTACTCTTGGAAGCTGGTTCATCCCACAGAC AAGTTCTGCAACAAGGACTGTCCTGGCACCGCGGAGGAATATGAGAGAGCCACACGCTACAATTACACCA GTGAGGAAAAATTTGCCTTCGTTGAGGTGATCGCCATGATCAAAGGCCTGCAGGTGCTCATGGGCAGGAT GGAGAGCGTCTTCAACCAGGCCATCAGGAACACCATCTACGCGGCATTGCAGGACTTCGCCCAGGTGACG CTGCGTGAGCCCCTGCGGCAGGCGGTACGGAAGAAGAAGAATGTCCTCATCAGCGTCCTACAGGCAATTC GAAAGACCATCTGTGACTGGGAGGGAGGGCGAGAGCCCCCTAATGACCCATGCTTGAGAGGGGAGAAGGA CCCCAAAGGTGGATTTGATATCAAGGTGCCCCGGCGTGCTGTGGGGCCATCCAGCACACAGGCCTGCCAG TGGTCCCCGAGGGCTTTGTTTCACCCCACTGGTGGCACACAGGGCCGAAGAGGCTGCCGATCCCTGCTGT ACATGGTGCGGACCATGCTTGAATCACTCATTGCAGACAAAAGCGGCTCCAAGAAGACCCTGAGGAGCAG CCTGGATGGACCCATTGTCCTCGCCATAGAGGACTTTCACAAACAGTCCTTCTTCTTCACACATCTGCTC AACATCAGTGAAGCCCTGCAGCAGTGTTGTGACCTCTCCCAGCTCTGGTTCCGAGAATTCTTCCTGGAGT TAACCATGGGCCGACGAATCCAGTTCCCCATCGAGATGTCCATGCCCTGGATTCTAACGGACCATATCCT GGAAACCAAAGAACCTTCCATGATGGAGTATGTCCTCTACCCTCTGGATCTGTACAACGACAGCGCCTAC TATGCTCTGACCAAGTTTAAAAAGCAGTTCCTGTACGATGAGATAGAAGCTGAGGTGAACCTGTGTTTTG ATCAGTTTGTCTACAAGCTGGCAGACCAGATCTTTGCTTACTACAAAGCCATGGCTGGCAGTGTCCTGTT GGATAAACGTTTTCGAGCTGAGTGTAAGAATTATGGCGTCATCATTCCGTATCCACCGTCCAATCGCTAT GAAACACTGCTGAAGCAGAGACACGTCCAGCTGTTGGGTAGATCAATTGACTTGAACAGACTCATTACCC AGCGCATCTCTGCCGCCATGTATAAATCCTTGGACCAAGCTATCAGCCGCTTTGAGAGTGAGGACCTGAC CTCCATTGTGGAGCTGGAGTGGCTGCTGGAGATTAACCGGCTCACGCATCGGCTGCTCTGTAAGCATATG ACGCTGGACAGCTTCGATGCCATGTTCCGAGAGGCCAATCACAATGTGTCCGCCCCCTATGGCCGTATCA CCCTGCATGTCTTCTGGGAACTGAACTTTGACTTTCTCCCCAACTACTGCTACAATGGGTCCACTAACCG TTTTGTGCGGACTGCCATTCCTTTCACCCAAGAACCACAACGAGACAAACCTGCCAACGTCCAGCCTTAT TACCTCTATGGATCCAAGCCTCTCAACATTGCCTACAGCCACATCTACAGCTCCTACAGGAATTTCGTGG GGCCACCTCATTTCAAGACTATCTGCAGACTCCTGGGTTATCAGGGCATCGCTGTGGTCATGGAGGAACT GCTAAAGATTGTGAAGAGCTTGCTCCAAGGAACCATTCTCCAGTATGTGAAAACACTGATAGAGGTGATG CCCAAGATATGCCGCTTGCCCCGACATGAGTATGGCTCCCCAGGGATCCTGGAGTTCTTCCACCACCAGC TGAAGGACATCATTGAGTACGCAGAGCTCAAAACAGACGTGTTCCAGAGCCTGAGGGAAGTGGGCAATGC CATCCTCTTCTGCCTCCTCATAGAGCAAGCTCTGTCTCAGGAGGAGGTCTGCGATTTGCTCCATGCCGCA CCCTTCCAAAACATCTTGCCTAGAGTCTACATCAAAGAGGGGGAGCGCCTGGAGGTCCGGATGAAACGTC TGGAAGCCAAGTATGCCCCGCTCCACCTGGTCCCTCTGATCGAGCGGCTGGGGACCCCTCAGCAAATCGC CATTGCTCGCGAGGGTGACCTCCTGACCAAGGAGCGGCTGTGCTGTGGCCTGTCCATGTTCGAGGTCATC CTGACCCGCATTCGGAGCTACCTGCAGGACCCCATCTGGCGGGGCCCACCGCCCACCAATGGCGTCATGC ACGTCGATGAGTGTGTGGAGTTCCACCGGCTGTGGAGCGCCATGCAGTTCGTGTACTGCATCCCTGTGGG AACCAACGAGTTCACAGCTGAGCAGTGTTTCGGCGATGGCTTGAACTGGGCTGGTTGCTCCATCATTGTC CTGCTGGGCCAGCAGCGTCGCTTTGACCTGTTCGACTTCTGTTACCACCTGCTAAAAGTGCAGAGGCAGG ACGGGAAGGATGAAATCATTAAGAATGTGCCCCTGAAGAAGATGGCCGACCGGATCAGGAAGTATCAGAT CTTGAACAATGAGGTTTTTGCCATCCTGAACAAATACATGAAGTCCGTGGAGACAGACAGTTCCACTGTG GAGCATGTGCGCTGCTTCCAGCCACCCATCCACCAGTCCTTGGCCACCACTTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC240089 representing NM_001291722
Red=Cloning site Green=Tags(s) MTTHVTLEDALSNVDLLEELPLPDQQPCIEPPPSSIMYQANFDTNFEDRNAFVTGIARYIEQATVHSSMN EMLEEGHEYAVMLYTWRSCSRAIPQVKCNEQPNRVEIYEKTVEVLEPEVTKLMKFMYFQRKAIERFCSEV KRLCHAERRKDFVSEAYLLTLGKFINMFAVLDELKNMKCSVKNDHSAYKRAAQFLRKMADPQSIQESQNL SMFLANHNRITQCLHQQLEVIPGYEELLADIVNICVDYYENKMYLTPSEKHMLLKVMGFGLYLMDGNVSN IYKLDAKKRINLSKIDKFFKQLQVVPLFGDMQIELARYIKTSAHYEENKSKWTCTQSSISPQYNICEQMV QIRDDHIRFISELARYSNSEVVTGSGLDSQKSDEEYRELFDLALRGLQLLSKWSAHVMEVYSWKLVHPTD KFCNKDCPGTAEEYERATRYNYTSEEKFAFVEVIAMIKGLQVLMGRMESVFNQAIRNTIYAALQDFAQVT LREPLRQAVRKKKNVLISVLQAIRKTICDWEGGREPPNDPCLRGEKDPKGGFDIKVPRRAVGPSSTQACQ WSPRALFHPTGGTQGRRGCRSLLYMVRTMLESLIADKSGSKKTLRSSLDGPIVLAIEDFHKQSFFFTHLL NISEALQQCCDLSQLWFREFFLELTMGRRIQFPIEMSMPWILTDHILETKEPSMMEYVLYPLDLYNDSAY YALTKFKKQFLYDEIEAEVNLCFDQFVYKLADQIFAYYKAMAGSVLLDKRFRAECKNYGVIIPYPPSNRY ETLLKQRHVQLLGRSIDLNRLITQRISAAMYKSLDQAISRFESEDLTSIVELEWLLEINRLTHRLLCKHM TLDSFDAMFREANHNVSAPYGRITLHVFWELNFDFLPNYCYNGSTNRFVRTAIPFTQEPQRDKPANVQPY YLYGSKPLNIAYSHIYSSYRNFVGPPHFKTICRLLGYQGIAVVMEELLKIVKSLLQGTILQYVKTLIEVM PKICRLPRHEYGSPGILEFFHHQLKDIIEYAELKTDVFQSLREVGNAILFCLLIEQALSQEEVCDLLHAA PFQNILPRVYIKEGERLEVRMKRLEAKYAPLHLVPLIERLGTPQQIAIAREGDLLTKERLCCGLSMFEVI LTRIRSYLQDPIWRGPPPTNGVMHVDECVEFHRLWSAMQFVYCIPVGTNEFTAEQCFGDGLNWAGCSIIV LLGQQRRFDLFDFCYHLLKVQRQDGKDEIIKNVPLKKMADRIRKYQILNNEVFAILNKYMKSVETDSSTV EHVRCFQPPIHQSLATTC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001291722 |
ORF Size | 3834 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001291722.2 |
RefSeq Size | 6574 bp |
RefSeq ORF | 3837 bp |
Locus ID | 26999 |
UniProt ID | Q96F07 |
Cytogenetics | 5q33.3 |
Protein Pathways | Regulation of actin cytoskeleton |
MW | 148.8 kDa |
Gene Summary | Involved in T-cell adhesion and p53/TP53-dependent induction of apoptosis. Does not bind RNA. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity).[UniProtKB/Swiss-Prot Function] |
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