SA2 (STAG2) (NM_001282418) Human Tagged ORF Clone
CAT#: RC240063
- TrueORF®
STAG2 (myc-DDK-tagged) - Human stromal antigen 2 (STAG2), transcript variant 5
ORF Plasmid: tGFP
"NM_001282418" in other vectors (2)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | SA2 |
Synonyms | bA517O1.1; HPE13; MKMS; NEDXCF; SA-2; SA2; SCC3B |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC240063 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATAGCAGCTCCAGAAATACCAACTGATTTTAATCTACTACAGGAGTCAGAAACACATTTTTCTTCTG ACACAGATTTTGAAGATATCGAAGGAAAAAACCAAAAGCAAGGCAAAGGCAAAACTTGTAAAAAAGGCAA AAAGGGCCCAGCAGAAAAGGGCAAAGGTGGAAATGGAGGAGGAAAACCTCCTTCTGGTCCAAACCGAATG AATGGTCATCACCAACAGAATGGAGTGGAAAACATGATGTTGTTTGAAGTTGTTAAAATGGGCAAGAGTG CTATGCAGTCGGTGGTAGATGATTGGATAGAATCATACAAGCATGACCGAGATATAGCACTTCTTGACCT TATCAACTTTTTTATTCAGTGTTCAGGCTGTAAAGGAGTTGTCACAGCAGAAATGTTTAGACATATGCAG AACTCTGAGATAATTCGAAAAATGACTGAAGAATTCGATGAGGATAGTGGAGATTATCCACTTACCATGG CTGGTCCTCAGTGGAAGAAGTTCAAATCCAGTTTTTGTGAATTCATTGGCGTGTTAGTACGGCAATGTCA ATATAGTATCATATATGATGAGTATATGATGGATACAGTCATTTCACTTCTTACAGGATTGTCTGACTCA CAAGTCAGAGCATTTCGACATACAAGCACCCTGGCAGCTATGAAGTTGATGACAGCTTTGGTGAATGTGG CACTAAATCTTAGCATTAATATGGATAATACACAAAGACAATATGAAGCAGAACGGAATAAAATGATTGG AAAACGAGCCAATGAGAGGCTAGAACTCCTGCTACAAAAGCGGAAAGAGCTTCAGGAAAATCAAGATGAA ATAGAAAATATGATGAATGCAATATTTAAAGGAGTGTTTGTACATAGATACCGTGATGCGATAGCTGAAA TTCGAGCTATTTGCATTGAAGAGATTGGCATTTGGATGAAGATGTATAGTGATGCCTTTCTTAATGACAG TTATTTAAAATATGTTGGTTGGACTATGCATGATAAGCAAGGTGAAGTAAGACTCAAATGTCTTACTGCT CTACAAGGGCTTTATTATAACAAAGAGCTTAATTCCAAACTGGAACTTTTTACCAGTCGGTTCAAGGATA GAATTGTGTCTATGACCCTTGACAAAGAATATGATGTTGCAGTACAAGCAATAAAATTACTCACTCTTGT TTTACAGAGTAGTGAAGAAGTTCTCACTGCAGAAGATTGTGAAAATGTCTATCATCTGGTTTATTCAGCT CACCGGCCAGTAGCAGTAGCAGCTGGAGAATTTCTCTACAAAAAGCTCTTCAGTCGTAGAGATCCAGAGG AGGATGGAATGATGAAAAGAAGAGGAAGACAAGGTCCAAATGCCAACCTTGTTAAGACATTGGTTTTTTT CTTTCTAGAAAGTGAGTTACATGAGCATGCAGCATACCTTGTGGATAGCATGTGGGACTGTGCTACTGAG CTGCTGAAAGACTGGGAATGTATGAATAGCTTGTTACTGGAAGAGCCACTTAGTGGAGAGGAAGCACTAA CAGATAGGCAAGAGAGTGCTCTGATTGAAATAATGCTTTGTACCATTAGACAAGCGGCTGAATGTCATCC TCCCGTGGGAAGAGGGACAGGAAAAAGGGTGCTTACAGCAAAGGAGAAGAAGACACAGTTGGATGATAGG ACAAAAATCACTGAGCTTTTTGCCGTGGCCCTTCCTCAGTTATTAGCAAAATACTCTGTAGATGCAGAAA AGGTGACTAACTTGTTGCAGTTGCCTCAGTACTTTGATTTGGAAATATATACCACTGGACGATTAGAAAA GCATTTGGATGCCTTATTGCGACAGATCCGGAATATTGTAGAGAAGCACACAGATACAGATGTTTTGGAA GCATGTTCTAAAACTTACCATGCACTCTGTAATGAAGAGTTCACAATCTTCAACAGAGTAGATATTTCAA GAAGTCAACTGATAGATGAATTGGCAGATAAATTTAACCGGCTTCTTGAAGATTTTCTGCAAGAGGGTGA AGAACCTGATGAAGATGATGCATATCAGGTATTGTCAACATTGAAGAGGATCACTGCTTTTCATAATGCC CATGACCTTTCAAAGTGGGATTTATTTGCTTGTAATTACAAACTCTTGAAAACTGGAATCGAAAATGGAG ACATGCCTGAGCAGATTGTTATTCACGCACTGCAGTGTACTCACTATGTAATCCTTTGGCAACTTGCTAA GATAACTGAAAGCAGCTCTACAAAGGAGGACTTGCTGCGTTTAAAGAAACAAATGAGAGTATTTTGTCAG ATATGTCAACATTACCTGACCAACGTGAATACTACTGTTAAGGAACAGGCCTTCACTATTCTGTGTGATA TTTTGATGATCTTCAGCCATCAGATTATGTCAGGAGGGCGTGACATGTTAGAGCCATTAGTGTATACCCC TGATTCTTCATTGCAGTCTGAGTTGCTCAGCTTTATTTTGGATCATGTCTTCATTGAACAGGATGATGAT AATAATAGTGCAGATGGTCAGCAAGAGGATGAAGCCAGTAAAATTGAAGCTCTGCACAAGAGAAGAAATT TACTTGCAGCATTTTGTAAGCTAATTGTATATACTGTGGTGGAGATGAATACAGCTGCAGATATCTTCAA ACAGTATATGAAGTATTATAATGACTATGGAGATATCATCAAAGAAACAATGAGTAAAACAAGGCAGATA GACAAAATTCAGTGTGCTAAGACCCTTATTCTCAGTCTGCAACAGCTTTTTAATGAAATGATACAAGAAA ATGGCTATAATTTTGATAGATCATCCTCTACATTTAGTGGCATAAAAGAACTTGCTCGACGTTTTGCTTT AACTTTTGGACTTGATCAGTTGAAAACAAGAGAAGCCATTGCCATGCTACACAAAGATGGCATAGAATTT GCTTTTAAAGAGCCTAATCCGCAAGGGGAGAGCCATCCACCTTTAAATTTGGCATTTCTTGATATTCTGA GTGAATTTTCTTCTAAACTACTTCGACAAGACAAAAGAACAGTGTATGTTTACTTGGAAAAGTTCATGAC CTTTCAGATGTCACTCCGAAGAGAGGATGTGTGGCTTCCACTGATGTCTTACCGAAATTCTTTGCTAGCT GGTGGTGATGATGACACCATGTCAGTCATTAGTGGAATCAGCAGCCGGGGGTCAACAGTACGGAGTAAAA AATCAAAACCATCTACAGGAAAACGGAAAGTGGTTGAGGGCATGCAGCTTTCACTCACTGAAGAAAGTAG TAGTAGTGACAGTATGTGGTTAAGCAGAGAACAAACACTGCACACCCCTGTTATGATGCAGACACCACAA CTCACCTCCACTATTATGAGAGAGCCCAAAAGATTACGGCCTGAGGATAGCTTCATGAGTGTTTATCCAA TGCAGACTGAACATCATCAAACACCTCTTGATTATAATCGGCGTGGCACAAGCCTAATGGAAGATGATGA AGAGCCAATTGTGGAAGATGTTATGATGTCCTCAGAAGGGAGGATTGAGGATCTTAATGAGGGAATGGAT TTTGACACCATGGATATAGATTTGCCACCATCAAAGAACAGACGAGAGAGAACAGAACTGAAGCCTGATT TCTTTGATCCAGCTTCAATTATGGATGAATCAGTTCTTGGAGTGTCAATGTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC240063 protein sequence
Red=Cloning site Green=Tags(s) MIAAPEIPTDFNLLQESETHFSSDTDFEDIEGKNQKQGKGKTCKKGKKGPAEKGKGGNGGGKPPSGPNRM NGHHQQNGVENMMLFEVVKMGKSAMQSVVDDWIESYKHDRDIALLDLINFFIQCSGCKGVVTAEMFRHMQ NSEIIRKMTEEFDEDSGDYPLTMAGPQWKKFKSSFCEFIGVLVRQCQYSIIYDEYMMDTVISLLTGLSDS QVRAFRHTSTLAAMKLMTALVNVALNLSINMDNTQRQYEAERNKMIGKRANERLELLLQKRKELQENQDE IENMMNAIFKGVFVHRYRDAIAEIRAICIEEIGIWMKMYSDAFLNDSYLKYVGWTMHDKQGEVRLKCLTA LQGLYYNKELNSKLELFTSRFKDRIVSMTLDKEYDVAVQAIKLLTLVLQSSEEVLTAEDCENVYHLVYSA HRPVAVAAGEFLYKKLFSRRDPEEDGMMKRRGRQGPNANLVKTLVFFFLESELHEHAAYLVDSMWDCATE LLKDWECMNSLLLEEPLSGEEALTDRQESALIEIMLCTIRQAAECHPPVGRGTGKRVLTAKEKKTQLDDR TKITELFAVALPQLLAKYSVDAEKVTNLLQLPQYFDLEIYTTGRLEKHLDALLRQIRNIVEKHTDTDVLE ACSKTYHALCNEEFTIFNRVDISRSQLIDELADKFNRLLEDFLQEGEEPDEDDAYQVLSTLKRITAFHNA HDLSKWDLFACNYKLLKTGIENGDMPEQIVIHALQCTHYVILWQLAKITESSSTKEDLLRLKKQMRVFCQ ICQHYLTNVNTTVKEQAFTILCDILMIFSHQIMSGGRDMLEPLVYTPDSSLQSELLSFILDHVFIEQDDD NNSADGQQEDEASKIEALHKRRNLLAAFCKLIVYTVVEMNTAADIFKQYMKYYNDYGDIIKETMSKTRQI DKIQCAKTLILSLQQLFNEMIQENGYNFDRSSSTFSGIKELARRFALTFGLDQLKTREAIAMLHKDGIEF AFKEPNPQGESHPPLNLAFLDILSEFSSKLLRQDKRTVYVYLEKFMTFQMSLRREDVWLPLMSYRNSLLA GGDDDTMSVISGISSRGSTVRSKKSKPSTGKRKVVEGMQLSLTEESSSSDSMWLSREQTLHTPVMMQTPQ LTSTIMREPKRLRPEDSFMSVYPMQTEHHQTPLDYNRRGTSLMEDDEEPIVEDVMMSSEGRIEDLNEGMD FDTMDIDLPPSKNRRERTELKPDFFDPASIMDESVLGVSMF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001282418 |
ORF Size | 3693 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001282418.2 |
RefSeq Size | 6047 bp |
RefSeq ORF | 3696 bp |
Locus ID | 10735 |
UniProt ID | Q8N3U4 |
Cytogenetics | Xq25 |
Protein Pathways | Cell cycle |
MW | 141.3 kDa |
Gene Summary | The protein encoded by this gene is a subunit of the cohesin complex, which regulates the separation of sister chromatids during cell division. Targeted inactivation of this gene results in chromatid cohesion defects and aneuploidy, suggesting that genetic disruption of cohesin is a cause of aneuploidy in human cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013] |
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