CYFIP2 (NM_001291721) Human Tagged ORF Clone
CAT#: RC240058
- TrueORF®
CYFIP2 (myc-DDK-tagged) - Human cytoplasmic FMR1 interacting protein 2 (CYFIP2), transcript variant 4
ORF Plasmid: tGFP
"NM_001291721" in other vectors (2)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | CYFIP2 |
Synonyms | EIEE65; PIR121 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC240058 representing NM_001291721
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCACGCACGTCACCCTGGAAGATGCCCTGTCCAACGTGGACCTGCTTGAAGAGCTTCCCCTCCCCG ACCAGCAGCCATGCATCGAGCCTCCACCTTCCTCCATCATGTACCAGGCTAACTTTGACACAAACTTTGA GGACAGGAATGCATTTGTCACGGGCATTGCAAGGTACATTGAGCAGGCTACAGTCCACTCCAGCATGGTG AAATGCAACGAGCAGCCCAACCGAGTAGAGATCTATGAGAAGACAGTAGAGGTGCTGGAGCCGGAGGTCA CCAAGCTCATGAAGTTCATGTATTTTCAGCGCAAGGCCATCGAGCGGTTCTGCAGCGAGGTGAAGCGGCT GTGCCATGCCGAGCGCAGGAAGGACTTTGTCTCTGAGGCCTACCTCCTGACCCTTGGCAAGTTCATCAAC ATGTTTGCTGTCCTGGATGAGCTAAAGAACATGAAGTGCAGCGTCAAGAATGACCACTCTGCCTACAAGA GGGCAGCACAGTTCCTGCGGAAGATGGCAGATCCCCAGTCTATCCAGGAGTCGCAGAACCTTTCCATGTT CCTGGCCAACCACAACAGGATCACCCAGTGTCTCCACCAGCAACTTGAAGTGATCCCAGGCTATGAGGAG CTGCTGGCTGACATTGTCAACATCTGTGTGGATTACTACGAGAACAAGATGTACCTGACTCCCAGTGAGA AACATATGCTCCTCAAGGTGATGGGCTTTGGCCTCTACCTAATGGATGGAAATGTCAGTAACATTTACAA ACTGGATGCCAAGAAGAGAATTAATCTTAGCAAAATTGATAAATTCTTTAAGCAGCTGCAGGTGGTGCCC CTTTTCGGCGACATGCAGATAGAGCTGGCCAGATACATTAAGACCAGTGCTCACTATGAAGAGAACAAGT CCAAGTGGACGTGCACCCAGAGCAGCATCAGCCCCCAGTACAATATCTGCGAGCAGATGGTTCAGATCCG GGATGACCACATCCGCTTCATCTCCGAGCTCGCTCGCTACAGCAACAGTGAGGTGGTGACGGGCTCAGGG CTGGACAGCCAGAAGTCAGACGAGGAGTATCGCGAGCTCTTCGACCTAGCCCTGCGGGGTCTGCAGCTTC TATCCAAGTGGAGCGCCCACGTCATGGAGGTGTACTCTTGGAAGCTGGTTCATCCCACAGACAAGTTCTG CAACAAGGACTGTCCTGGCACCGCGGAGGAATATGAGAGAGCCACACGCTACAATTACACCAGTGAGGAA AAATTTGCCTTCGTTGAGGTGATCGCCATGATCAAAGGCCTGCAGGTGCTCATGGGCAGGATGGAGAGCG TCTTCAACCAGGCCATCAGGAACACCATCTACGCGGCATTGCAGGACTTCGCCCAGGTGACGCTGCGTGA GCCCCTGCGGCAGGCGGTACGGAAGAAGAAGAATGTCCTCATCAGCGTCCTACAGGCAATTCGAAAGACC ATCTGTGACTGGGAGGGAGGGCGAGAGCCCCCTAATGACCCATGCTTGAGAGGGGAGAAGGACCCCAAAG GTGGATTTGATATCAAGGTGCCCCGGCGTGCTGTGGGGCCATCCAGCACACAGCTGTACATGGTGCGGAC CATGCTTGAATCACTCATTGCAGACAAAAGCGGCTCCAAGAAGACCCTGAGGAGCAGCCTGGATGGACCC ATTGTCCTCGCCATAGAGGACTTTCACAAACAGTCCTTCTTCTTCACACATCTGCTCAACATCAGTGAAG CCCTGCAGCAGTGTTGTGACCTCTCCCAGCTCTGGTTCCGAGAATTCTTCCTGGAGTTAACCATGGGCCG ACGAATCCAGTTCCCCATCGAGATGTCCATGCCCTGGATTCTAACGGACCATATCCTGGAAACCAAAGAA CCTTCCATGATGGAGTATGTCCTCTACCCTCTGGATCTGTACAACGACAGCGCCTACTATGCTCTGACCA AGTTTAAAAAGCAGTTCCTGTACGATGAGATAGAAGCTGAGGTGAACCTGTGTTTTGATCAGTTTGTCTA CAAGCTGGCAGACCAGATCTTTGCTTACTACAAAGCCATGGCTGGCAGTGTCCTGTTGGATAAACGTTTT CGAGCTGAGTGTAAGAATTATGGCGTCATCATTCCGTATCCACCGTCCAATCGCTATGAAACACTGCTGA AGCAGAGACACGTCCAGCTGTTGGGTAGATCAATTGACTTGAACAGACTCATTACCCAGCGCATCTCTGC CGCCATGTATAAATCCTTGGACCAAGCTATCAGCCGCTTTGAGAGTGAGGACCTGACCTCCATTGTGGAG CTGGAGTGGCTGCTGGAGATTAACCGGCTCACGCATCGGCTGCTCTGTAAGCATATGACGCTGGACAGCT TCGATGCCATGTTCCGAGAGGCCAATCACAATGTGTCCGCCCCCTATGGCCGTATCACCCTGCATGTCTT CTGGGAACTGAACTTTGACTTTCTCCCCAACTACTGCTACAATGGGTCCACTAACCGTTTTGTGCGGACT GCCATTCCTTTCACCCAAGAACCACAACGAGACAAACCTGCCAACGTCCAGCCTTATTACCTCTATGGAT CCAAGCCTCTCAACATTGCCTACAGCCACATCTACAGCTCCTACAGGAATTTCGTGGGGCCACCTCATTT CAAGACTATCTGCAGACTCCTGGGTTATCAGGGCATCGCTGTGGTCATGGAGGAACTGCTAAAGATTGTG AAGAGCTTGCTCCAAGGAACCATTCTCCAGTATGTGAAAACACTGATAGAGGTGATGCCCAAGATATGCC GCTTGCCCCGACATGAGTATGGCTCCCCAGGGATCCTGGAGTTCTTCCACCACCAGCTGAAGGACATCAT TGAGTACGCAGAGCTCAAAACAGACGTGTTCCAGAGCCTGAGGGAAGTGGGCAATGCCATCCTCTTCTGC CTCCTCATAGAGCAAGCTCTGTCTCAGGAGGAGGTCTGCGATTTGCTCCATGCCGCACCCTTCCAAAACA TCTTGCCTAGAGTCTACATCAAAGAGGGGGAGCGCCTGGAGGTCCGGATGAAACGTCTGGAAGCCAAGTA TGCCCCGCTCCACCTGGTCCCTCTGATCGAGCGGCTGGGGACCCCTCAGCAAATCGCCATTGCTCGCGAG GGTGACCTCCTGACCAAGGAGCGGCTGTGCTGTGGCCTGTCCATGTTCGAGGTCATCCTGACCCGCATTC GGAGCTACCTGCAGGACCCCATCTGGCGGGGCCCACCGCCCACCAATGGCGTCATGCACGTCGATGAGTG TGTGGAGTTCCACCGGCTGTGGAGCGCCATGCAGTTCGTGTACTGCATCCCTGTGGGAACCAACGAGTTC ACAGCTGAGCAGTGTTTCGGCGATGGCTTGAACTGGGCTGGTTGCTCCATCATTGTCCTGCTGGGCCAGC AGCGTCGCTTTGACCTGTTCGACTTCTGTTACCACCTGCTAAAAGTGCAGAGGCAGGACGGGAAGGATGA AATCATTAAGAATGTGCCCCTGAAGAAGATGGCCGACCGGATCAGGAAGTATCAGATCTTGAACAATGAG GTTTTTGCCATCCTGAACAAATACATGAAGTCCGTGGAGACAGACAGTTCCACTGTGGAGCATGTGCGCT GCTTCCAGCCACCCATCCACCAGTCCTTGGCCACCACTTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC240058 representing NM_001291721
Red=Cloning site Green=Tags(s) MTTHVTLEDALSNVDLLEELPLPDQQPCIEPPPSSIMYQANFDTNFEDRNAFVTGIARYIEQATVHSSMV KCNEQPNRVEIYEKTVEVLEPEVTKLMKFMYFQRKAIERFCSEVKRLCHAERRKDFVSEAYLLTLGKFIN MFAVLDELKNMKCSVKNDHSAYKRAAQFLRKMADPQSIQESQNLSMFLANHNRITQCLHQQLEVIPGYEE LLADIVNICVDYYENKMYLTPSEKHMLLKVMGFGLYLMDGNVSNIYKLDAKKRINLSKIDKFFKQLQVVP LFGDMQIELARYIKTSAHYEENKSKWTCTQSSISPQYNICEQMVQIRDDHIRFISELARYSNSEVVTGSG LDSQKSDEEYRELFDLALRGLQLLSKWSAHVMEVYSWKLVHPTDKFCNKDCPGTAEEYERATRYNYTSEE KFAFVEVIAMIKGLQVLMGRMESVFNQAIRNTIYAALQDFAQVTLREPLRQAVRKKKNVLISVLQAIRKT ICDWEGGREPPNDPCLRGEKDPKGGFDIKVPRRAVGPSSTQLYMVRTMLESLIADKSGSKKTLRSSLDGP IVLAIEDFHKQSFFFTHLLNISEALQQCCDLSQLWFREFFLELTMGRRIQFPIEMSMPWILTDHILETKE PSMMEYVLYPLDLYNDSAYYALTKFKKQFLYDEIEAEVNLCFDQFVYKLADQIFAYYKAMAGSVLLDKRF RAECKNYGVIIPYPPSNRYETLLKQRHVQLLGRSIDLNRLITQRISAAMYKSLDQAISRFESEDLTSIVE LEWLLEINRLTHRLLCKHMTLDSFDAMFREANHNVSAPYGRITLHVFWELNFDFLPNYCYNGSTNRFVRT AIPFTQEPQRDKPANVQPYYLYGSKPLNIAYSHIYSSYRNFVGPPHFKTICRLLGYQGIAVVMEELLKIV KSLLQGTILQYVKTLIEVMPKICRLPRHEYGSPGILEFFHHQLKDIIEYAELKTDVFQSLREVGNAILFC LLIEQALSQEEVCDLLHAAPFQNILPRVYIKEGERLEVRMKRLEAKYAPLHLVPLIERLGTPQQIAIARE GDLLTKERLCCGLSMFEVILTRIRSYLQDPIWRGPPPTNGVMHVDECVEFHRLWSAMQFVYCIPVGTNEF TAEQCFGDGLNWAGCSIIVLLGQQRRFDLFDFCYHLLKVQRQDGKDEIIKNVPLKKMADRIRKYQILNNE VFAILNKYMKSVETDSSTVEHVRCFQPPIHQSLATTC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001291721 |
ORF Size | 3681 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001291721.2 |
RefSeq Size | 6421 bp |
RefSeq ORF | 3684 bp |
Locus ID | 26999 |
UniProt ID | Q96F07 |
Cytogenetics | 5q33.3 |
Protein Pathways | Regulation of actin cytoskeleton |
MW | 143 kDa |
Gene Summary | Involved in T-cell adhesion and p53/TP53-dependent induction of apoptosis. Does not bind RNA. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity).[UniProtKB/Swiss-Prot Function] |
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