TReP132 (TRERF1) (NM_001297574) Human Tagged ORF Clone

CAT#: RC240053

TRERF1 (myc-DDK-tagged) - Human transcriptional regulating factor 1 (TRERF1), transcript variant 1

Product Images

Circular map for RC240053

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TReP132
• Synonyms: BCAR2; dJ139D8.5; HSA277276; RAPA; TReP-132; TREP132
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC240053 representing NM_001297574
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGACCAGCAACTGTACAAGACCAACCATGTGGCCCATGGTAGTGAGAACCTTTTCTACCAACAGC
  CACCACTTGGCGTCCACAGCGGGCTGAACCACAACTATGGGAATGCAGTTACAGGGGGCGGAATGGATGC
  CCCTCAGGCCTCGCCAATCTCCCCCCACTTCCCTCAAGATACACGGGATGGTCTGGGCTTGCCTGTTGGC
  TCCAAAAACCTTGGCCAAATGGATACCTCGAGGCAGGGAGGGTGGGGAAGTCATGCAGGGCCTGGAAACC
  ATGTCCAGCTACGTGGAAACCTGGCCAACTCAAACATGATGTGGGGGGCACCAGCCCAGGCTGAGCCCAC
  TGATGGCTACCAATACACCTACTCCCAGGCCAGCGAGATCCGGACCCAGAAGCTTACCAGCGGTGTCTTA
  CACAAGCTGGACTCTTTCACCCAGGTGTTTGCCAACCAAAACCTGCGAATTCAGGTCAACAATATGGCCC
  AGGTGCTGCACACTCAGTCAGCAGTGATGGATGGAGCCCCTGACAGTGCTCTCCGCCAGCTGCTGTCTCA
  GAAGCCCATGGAGCCCCCAGCACCGGCTATCCCTTCCCGCTACCAGCAGGTGCCCCAGCAGCCTCACCCT
  GGTTTCACTGGTGGGCTGTCCAAACCAGCTCTTCAGGTCGGGCAGCACCCTACCCAAGGGCACCTGTATT
  ATGACTACCAGCAGCCTCTGGCTCAGGTGCCAGTGCAGGGAGGACAGCCACTGCAGGCCCCACAGATGCT
  GTCACAGCACATGCAACAGATGCAGCAGCACCAGTATTACCCACCGCAGCAACAGCAGCAAGCCGGGCAA
  CAGCGTATCTCCATGCAAGAAATACAGACGCAGCCGCAACAAATTCGCCCATCACAGCCACAGCCGCCGC
  CACAGCAGCAGCAGCCGCAGCAGCTACAGCTGCAGCAGCGGCAGGGTTCAATGCAGATACCTCAGTATTA
  TCAGCCCCAACCCATGATGCAGCACTTGCAAGAGCAGCAGCAGCAACAGATGCACCTGCAGCCTCCTTCT
  TATCACAGGGACCCTCACCAGTATACCCCAGAGCAGGCACACACTGTCCAGCTGATTCCCCTGGGCTCCA
  TGTCCCAGTACTACTACCAGGAGCCCCAGCAGCCCTACAGCCACCCCCTCTACCAGCAGAGCCACCTGTC
  CCAGCACCAGCAGCGTGAGGACAGTCAGCTGAAGACCTACTCTAGTGACAGACAGGCCCAGGCCATGCTG
  AGCTCCCATGGGGACCTGGGGCCTCCTGACACAGGAATGGGAGACCCAGCGAGCTCAGATCTGACCCGGG
  TCAGCAGCACCCTCCCCCATCGCCCCCTCCTATCCCCCAGTGGGATCCACCTCAACAACATGGGGCCTCA
  GCATCAGCAGCTGTCTCCCAGTGCCATGTGGCCCCAGATGCACCTACCTGATGGGAGAGCCCAGCCAGGG
  TCCCCTGAGTCAAGTGGCCAACCCAAAGGAGCGTTTGGGGAGCAGTTTGATGCCAAGAACAAGCTGACAT
  GCTCCATCTGCCTGAAGGAGTTCAAGAACCTGCCTGCCCTGAATGGCCACATGCGGTCCCACGGGGGAAT
  GAGGGCCTCCCCCAACCTCAAACAGGAGGAAGGAGAGAAGGTCCTGCCGCCTCAGCCCCAGCCACCACTG
  CCGCCTCCGCCTCCGCCTCCGCCGCCACCACAGCTCCCTCCCGAGGCAGAAAGCCTCACGCCTATGGTCA
  TGCCCGTGTCTGTCCCTGTCAAGCTTCTCCCGCCCAAGCCCAGCTCTCAGGGGTTCACCAACAGCACCGT
  TGCCGCCCCCTCCGCCAGAGACAAGCCAGCCAGCTCGATGTCGGACGACGAGATGCCTGTGCTCGTGAGG
  ATGACCCTCTCTCCCCCACACTCACCCCAAGGGGCTGCCCCCCGCACGCCTGCTGAAATCCCCAGGAAGC
  ATCAGCCGAGTGTGCCCAAAGCCGAGGAGCCCCTCAAGACCGTGCAGGAGAAGAAAAAGTTCCGGCACCG
  GCCGGAACCTCTCTTCATCCCGCCGCCGCCCTCCTACAACCCGAACCCCGCTGCCTCCTACTCGGGCGCC
  ACCCTGTACCAGAGCCAGCTGCGCTCCCCGCGCGTCCTCGGGGACCACCTGCTCCTGGACCCCACCCACG
  AGCTGCCCCCTTACACGCCCCCACCCATGCTGAGCCCGGTGCGCCAGGGCTCGGGGCTCTTCAGCAATGT
  CCTCATCTCCGGCCACGGCCCTGGCGCCCACCCGCAGCTGCCCCTGACGCCCCTGACGCCCACACCACGG
  GTGCTGCTGTGTCGCTCCAACAGCATCGATGGCAGCAACGTGACGGTCACCCCAGGGCCTGGAGAGCAGA
  CTGTAGATGTTGAACCACGCATCAACATTGGCTTGAGATTCCAAGCAGAAATCCCTGAACTCCAAGATAT
  CTCTGCCCTGGCCCAGGACACACACAAGGCCACACTGGTATGGAAGCCCTGGCCAGAACTAGAAAACCAT
  GACCTCCAGCAAAGAGTGGAGAATCTTCTGAATTTGTGCTGTTCCAGTGCATTGCCAGGTGGAGGGACCA
  ATTCTGAATTTGCTTTGCACTCTCTGTTTGAGGCCAAAGGTGATGTGATGGTTGCTCTGGAAATGCTGCT
  ACTGCGGAAGCCTGTCAGGTTAAAATGTCATCCTTTAGCAAATTACCACTATGCCGGTTCGGACAAGTGG
  ACCTCCCTAGAAAGAAAACTGTTTAACAAAGCACTAGCCACTTACAGCAAAGACTTTATTTTTGTACAGA
  AGATGGTGAAGTCCAAGACGGTGGCTCAGTGCGTGGAGTACTACTACACGTGGAAAAAGATCATGCGGCT
  GGGGCGGAAACACCGGACACGCCTGGCAGAAATCATCGACGATTGTGTGACAAGTGAAGAAGAAGAAGAG
  TTAGAGGAGGAGGAGGAGGAGGACCCGGAAGAAGATAGGAAATCCACAAAAGAAGAAGAGAGTGAGGTGC
  CGAAGTCCCCGGAGCCACCACCCGTCCCCGTCCTGGCTCCCACGGAGGGGCCGCCCCTGCAGGCCCTGGG
  CCAGCCCTCAGGCTCCTTCATCTGTGAAATGCCCAACTGTGGGGCTGTGTTCAGCTCCCGACAGGCACTG
  AATGGCCATGCCCGCATCCACGGGGGCACCAACCAGGTGACCAAGGCCCGAGGTGCCATCCCCTCTGGGA
  AGCAGAAGCCTGGTGGCACCCAGAGTGGGTACTGTTCGGTAAAGAGCTCACCCTCTCACAGCACCACCAG
  CGGCGAGACAGACCCCACCACCATCTTCCCCTGCAAGGAGTGTGGCAAAGTCTTCTTCAAGATCAAAAGC
  CGAAATGCACACATGAAAACTCACAGGCAGCAGGAGGAACAACAGAGGCAAAAGGCTCAGAAGGCGGCTT
  TTGCAGCTGAGATGGCAGCCACGATTGAGAGGACTACGGGGCCCGTGGGGGCGCCGGGGCTGCTGCCCCT
  GGACCAGCTGAGTCTGATCAAACCCATCAAGGATGTGGACATCCTCGACGACGACGTCGTCCAGCAGTTG
  GGAGGTGTCATGGAAGAGGCTGAAGTTGTGGACACCGATCTTCTCTTGGATGATCAAGATTCAGTCTTGC
  TTCAGGGTGACGCAGAACTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC240053 representing NM_001297574
  Red=Cloning site Green=Tags(s)
  
  MGDQQLYKTNHVAHGSENLFYQQPPLGVHSGLNHNYGNAVTGGGMDAPQASPISPHFPQDTRDGLGLPVG
  SKNLGQMDTSRQGGWGSHAGPGNHVQLRGNLANSNMMWGAPAQAEPTDGYQYTYSQASEIRTQKLTSGVL
  HKLDSFTQVFANQNLRIQVNNMAQVLHTQSAVMDGAPDSALRQLLSQKPMEPPAPAIPSRYQQVPQQPHP
  GFTGGLSKPALQVGQHPTQGHLYYDYQQPLAQVPVQGGQPLQAPQMLSQHMQQMQQHQYYPPQQQQQAGQ
  QRISMQEIQTQPQQIRPSQPQPPPQQQQPQQLQLQQRQGSMQIPQYYQPQPMMQHLQEQQQQQMHLQPPS
  YHRDPHQYTPEQAHTVQLIPLGSMSQYYYQEPQQPYSHPLYQQSHLSQHQQREDSQLKTYSSDRQAQAML
  SSHGDLGPPDTGMGDPASSDLTRVSSTLPHRPLLSPSGIHLNNMGPQHQQLSPSAMWPQMHLPDGRAQPG
  SPESSGQPKGAFGEQFDAKNKLTCSICLKEFKNLPALNGHMRSHGGMRASPNLKQEEGEKVLPPQPQPPL
  PPPPPPPPPPQLPPEAESLTPMVMPVSVPVKLLPPKPSSQGFTNSTVAAPSARDKPASSMSDDEMPVLVR
  MTLSPPHSPQGAAPRTPAEIPRKHQPSVPKAEEPLKTVQEKKKFRHRPEPLFIPPPPSYNPNPAASYSGA
  TLYQSQLRSPRVLGDHLLLDPTHELPPYTPPPMLSPVRQGSGLFSNVLISGHGPGAHPQLPLTPLTPTPR
  VLLCRSNSIDGSNVTVTPGPGEQTVDVEPRINIGLRFQAEIPELQDISALAQDTHKATLVWKPWPELENH
  DLQQRVENLLNLCCSSALPGGGTNSEFALHSLFEAKGDVMVALEMLLLRKPVRLKCHPLANYHYAGSDKW
  TSLERKLFNKALATYSKDFIFVQKMVKSKTVAQCVEYYYTWKKIMRLGRKHRTRLAEIIDDCVTSEEEEE
  LEEEEEEDPEEDRKSTKEEESEVPKSPEPPPVPVLAPTEGPPLQALGQPSGSFICEMPNCGAVFSSRQAL
  NGHARIHGGTNQVTKARGAIPSGKQKPGGTQSGYCSVKSSPSHSTTSGETDPTTIFPCKECGKVFFKIKS
  RNAHMKTHRQQEEQQRQKAQKAAFAAEMAATIERTTGPVGAPGLLPLDQLSLIKPIKDVDILDDDVVQQL
  GGVMEEAEVVDTDLLLDDQDSVLLQGDAEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001297574
• ORF Size: 3660 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001297574.1, NP_001284503.1
• RefSeq Size: 7644 bp
• RefSeq ORF: 3663 bp
• Locus ID: 55809
• Cytogenetics: 6p21.1
• Protein Families: Druggable Genome, Transcription Factors
• MW: 134.8 kDa
• Gene Summary: This gene encodes a zinc-finger transcriptional regulating protein which interacts with CBP/p300 to regulate the human gene CYP11A1. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]