CACHD1 (NM_001293274) Human Tagged ORF Clone

CAT#: RC239847

CACHD1 (myc-DDK-tagged) - Human cache domain containing 1 (CACHD1), transcript variant 2

Product Images

Circular map for RC239847

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CACHD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239847 representing NM_001293274
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACCTTTGTTAGCAGCGTGAAGTCTTCAGACAGTCCTACCCAGCACGCAGTGGGATTCCAAAAGG
  CATTTCAGCTGATTCGAAGTACAAACAATAACACAAAGTTCCAAGCAAATACAGACATGGTCATCATTTA
  CCTGTCAGCTGGCATTACATCAAAGGACTCTTCGGAAGAAGATAAAAAAGCGACTCTCCAAGTCATCAAT
  GAAGAAAATAGCTTTCTAAACAACTCTGTAATGATTCTCACCTATGCCCTCATGAACGATGGGGTGACTG
  GTTTGAAAGAGCTGGCTTTTCTGAGGGATCTAGCTGAACAGAATTCAGGGAAGTACGGTGTGCCAGACCG
  GATGGCCTTGCCTGTGATTAAGGGCAGCATGATGGTGCTGAATCAGTTGAGCAACCTGGAGACCACAGTG
  GGCAGGTTCTACACAAACCTTCCCAACCGGATGATTGATGAAGCCGTCTTCAGCCTGCCCTTCTCTGATG
  AGATGGGAGATGGTTTGATAATGACTGTGAGTAAACCCTGTTATTTTGGAAACCTACTTCTGGGAATTGT
  AGGTGTGGACGTGAATCTGGCTTACATTCTTGAAGACGTGACGTATTACCAAGACTCTTTGGCTTCCTAT
  ACTTTTCTCATAGACGACAAAGGATATACACTTATGCACCCATCTCTTACCAGGCCATATTTATTGTCAG
  AGCCCCCACTTCATACTGACATCATACATTATGAAAATATTCCAAAATTTGAATTAGTTCGGCAAAATAT
  CCTAAGCCTCCCTCTGGGCAGCCAGATTATCGCAGTCCCTGTGAACTCATCCCTGTCTTGGCACATAAAC
  AAGCTGAGAGAAACTGGAAAGGAAGCCTACAATGTTAGCTATGCCTGGAAGATGGTACAAGACACTTCCT
  TTATTCTGTGTATTGTGGTGATACAACCAGAAATACCTGTGAAACAACTGAAGAACCTCAACACTGTTCC
  CAGCAGCAAGCTGCTGTACCACCGGCTGGATCTCCTTGGCCAGCCCAGTGCTTGCCTCCACTTCAAACAG
  CTGGCAACCCTAGAAAGTCCCACCATCATGCTGTCTGCTGGCAGCTTTTCCTCCCCCTATGAGCACCTCA
  GCCAGCCAGAGACAAAGCGCATGGTAGAGCACTACACCGCCTATCTCAGCGACAACACCCGCCTCATTGC
  TAACCCGGGCCTCAAATTCTCTGTCAGAAATGAAGTAATGGCTACCAGCCACGTCACAGATGAATGGATG
  ACACAAATGGAAATGAGTAGCCTGAACACTTACATTGTCCGCCGTTACATAGCAACACCCAATGGCGTCC
  TCAGAATTTATCCTGGTTCCCTCATGGACAAAGCATTTGATCCCACTAGGAGACAATGGTATCTCCATGC
  AGTAGCTAATCCAGGGTTGATTTCTTTGACTGGTCCTTACTTAGATGTTGGAGGAGCTGGTTATGTTGTG
  ACAATCAGTCACACAATTCATTCATCCAGTACACAGCTGTCTTCTGGGCACACTGTGGCTGTGATGGGCA
  TTGACTTCACACTCAGATACTTCTACAAAGTTCTGATGGACCTATTACCTGTCTGTAACCAAGATGGTGG
  CAACAAAATAAGGTGCTTCATAATGGAGGACAGGGGTTATCTGGTGGCGCACCCGACTCTCATCGACCCC
  AAAGGACATGCACCTGTGGAGCAGCAGCACATCACCCACAAGGAGCCCCTGGTAGCAAATGATATCCTCA
  ACCACCCCAACTTTGTAAAGAAAAACCTGTGCAACAGCTTCAGTGACAGAACGGTCCAGAGGTTTTATAA
  ATTCAACACCAGCCTTGCGGGGGATTTGACGAACCTTGTGCATGGCAGCCACTGTTCCAAATACAGATTA
  GCAAGGATCCCAGGAACCAACGCGTTTGTTGGCATTGTCAACGAAACCTGCGACTCTCTTGCCTTCTGTG
  CCTGCAGCATGGTGGACCGACTCTGTCTCAACTGTCACCGAATGGAACAAAATGAATGTGAATGTCCTTG
  TGAGTGCCCTCTAGAGGTCAATGAGTGCACTGGCAACCTCACCAATGCAGAGAACCGAAACCCCAGCTGC
  GAGGTCCACCAGGAGCCGGTGACATACACAGCTATTGACCCTGGCCTGCAAGATGCTCTTCACCAGTGTG
  TCAACAGCAGGTGCAGTCAGAGGCTGGAAAGTGGGGACTGTTTTGGGGTGCTGGATTGTGAATGGTGCAT
  GGTGGACAGTGATGGAAAGACTCACCTGGACAAACCCTACTGTGCCCCCCAGAAAGAATGCTTCGGGGGG
  ATTGTGGGAGCCAAAAGTCCCTACGTTGATGACATGGGAGCAATAGGTGATGAGGTGATCACATTAAACA
  TGATTAAAAGCGCCCCTGTGGGTCCTGTGGCTGGAGGGATCATGGGATGCATCATGGTCTTGGTCCTGGC
  GGTGTATGCCTACCGCCACCAGATTCATCGCCGGAGCCATCAGCATATGTCTCCTCTTGCTGCCCAAGAA
  ATGTCAGTGCGTATGTCCAACCTGGAGAATGACAGAGATGAAAGGGACGACGACAGCCACGAAGACAGAG
  GCATCATCAGCAACACTCGGTTTATAGCTGCGGTCATCGAACGACATGCACACAGTCCAGAAAGAAGGCG
  CCGCTACTGGGGTCGATCAGGAACAGAAAGTGATCATGGTTACAGCACCATGAGCCCACAGGAGGACAGT
  GAAAATCCTCCATGCAACAATGACCCCTTGTCAGCCGGGGTCGATGTGGGAAACCATGATGAGGACTTAG
  ACCTGGATACCCCCCCTCAGACTGCTGCCCTACTAAGTCACAAGTTCCACCACTACCGGTCACACCACCC
  TACACTTCATCATAGCCACCACTTACAGGCGGCCGTCACGGTACACACTGTCGATGCAGAATGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239847 representing NM_001293274
  Red=Cloning site Green=Tags(s)
  
  MSTFVSSVKSSDSPTQHAVGFQKAFQLIRSTNNNTKFQANTDMVIIYLSAGITSKDSSEEDKKATLQVIN
  EENSFLNNSVMILTYALMNDGVTGLKELAFLRDLAEQNSGKYGVPDRMALPVIKGSMMVLNQLSNLETTV
  GRFYTNLPNRMIDEAVFSLPFSDEMGDGLIMTVSKPCYFGNLLLGIVGVDVNLAYILEDVTYYQDSLASY
  TFLIDDKGYTLMHPSLTRPYLLSEPPLHTDIIHYENIPKFELVRQNILSLPLGSQIIAVPVNSSLSWHIN
  KLRETGKEAYNVSYAWKMVQDTSFILCIVVIQPEIPVKQLKNLNTVPSSKLLYHRLDLLGQPSACLHFKQ
  LATLESPTIMLSAGSFSSPYEHLSQPETKRMVEHYTAYLSDNTRLIANPGLKFSVRNEVMATSHVTDEWM
  TQMEMSSLNTYIVRRYIATPNGVLRIYPGSLMDKAFDPTRRQWYLHAVANPGLISLTGPYLDVGGAGYVV
  TISHTIHSSSTQLSSGHTVAVMGIDFTLRYFYKVLMDLLPVCNQDGGNKIRCFIMEDRGYLVAHPTLIDP
  KGHAPVEQQHITHKEPLVANDILNHPNFVKKNLCNSFSDRTVQRFYKFNTSLAGDLTNLVHGSHCSKYRL
  ARIPGTNAFVGIVNETCDSLAFCACSMVDRLCLNCHRMEQNECECPCECPLEVNECTGNLTNAENRNPSC
  EVHQEPVTYTAIDPGLQDALHQCVNSRCSQRLESGDCFGVLDCEWCMVDSDGKTHLDKPYCAPQKECFGG
  IVGAKSPYVDDMGAIGDEVITLNMIKSAPVGPVAGGIMGCIMVLVLAVYAYRHQIHRRSHQHMSPLAAQE
  MSVRMSNLENDRDERDDDSHEDRGIISNTRFIAAVIERHAHSPERRRRYWGRSGTESDHGYSTMSPQEDS
  ENPPCNNDPLSAGVDVGNHDEDLDLDTPPQTAALLSHKFHHYRSHHPTLHHSHHLQAAVTVHTVDAEC
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001293274
• ORF Size: 2934 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001293274.1, NP_001280203.1
• RefSeq Size: 5553 bp
• RefSeq ORF: 2937 bp
• Locus ID: 57685
• UniProt ID: Q5VU97
• Cytogenetics: 1p31.3
• Protein Families: Druggable Genome, Transmembrane
• MW: 109.6 kDa
• Gene Summary: May regulate voltage-dependent calcium channels.[UniProtKB/Swiss-Prot Function]