RPS6KC1 (NM_001287218) Human Tagged ORF Clone

CAT#: RC239688

RPS6KC1 (myc-DDK-tagged) - Human ribosomal protein S6 kinase, 52kDa, polypeptide 1 (RPS6KC1), transcript variant 3

Product Images

Circular map for RC239688

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RPS6KC1
• Synonyms: humS6PKh1; RPK118; RSKL1; S6K-delta-1; S6PKh1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239688 representing NM_001287218
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCCAATCAAAATTCTCCCATTAGAACTTTTGGTCTCAATCTTTCTTCGGATTCTTCAGCACTAG
  GGGCTGTTGCTTCTGACAGTGAACAGAGCAAAACAGAAGAAGAACGGGAAAGTCGTAGCCTCTTTCCTGG
  CAGTTTAAAGCCGAAGCTTGGCAAGAGAGATTATTTGGAGAAAGCAGGAGAATTAATAAAGCTGGCTTTA
  AAAAAGGAAGAAGAAGACGACTATGAAGCTGCTTCTGATTTTTATAGGAAGGGAGTTGATTTACTCCTAG
  AAGGTGTTCAAGGAGAGTCAAGCCCTACCCGTCGAGAAGCTGTGAAGAGAAGAACAGCCGAGTACCTCAT
  GCGGGCAGAAAGTATCTCTAGTCTTTATGGGAAACCTCAGCTTGATGATGTATCTCAGGTTTTACTTGTA
  ATGGACACAAGGACAGAACAGACTTTCATTTTAAAAGGTCTAAGGAAAAGCAGTGAATACAGCAGGAACA
  GAAAGACCATCATCCCCCGCTGTGTGCCCAACATGGTGTGTCTGCATAAGTACATCATCTCTGAGGAGTC
  AGTATTTCTTGTGCTGCAGCATGCGGAAGGTGGCAAACTGTGGTCATATATCAGTAAATTTCTAAACAGA
  AGTCCTGAAGAAAGCTTTGACATCAAGGAAGTGAAAAAACCTACACTTGCAAAAGTTCACCTGCAGCAGC
  CAACTTCTAGTCCTCAGGACAGCAGTAGCTTTGAATCCAGAGGAAGTGATGGTGGAAGCATGCTTAAAGC
  TCTGCCTTTGAAGAGTAGTCTTACTCCAAGTTCTCAAGATGACAGCAACCAGGAAGATGATGGCCAAGAT
  AGCTCTCCAAAGTGGCCAGATTCTGGTTCAAGTTCAGAAGAAGAATGTACTACTAGTTATTTAACATTAT
  GCAATGAATATGGGCAAGAAAAGATTGAACCAGGGTCTTTGAATGAGGAGCCCTTCATGAAGACTGAAGG
  GAATGGTGTTGATACAAAAGCTATTAAAAGCTTCCCAGCACACCTTGCTGCTGACAGTGACAGCCCCAGC
  ACACAGCTGAGAGCTCACGAGCTGAAGTTCTTCCCCAACGATGACCCAGAAGCAGTTAGTTCTCCAAGAA
  CATCAGATTCCCTCAGTAGATCAAAAAATAGCCCCATGGAATTCTTTAGGATAGACAGTAAGGATAGCGC
  AAGTGAACTCCTGGGACTTGACTTTGGAGAAAAATTGTATAGTCTAAAATCAGAACCTTTGAAACCATTC
  TTTACTCTTCCAGATGGAGACAGTGCTTCTAGGAGTTTTAATACTAGTGAAAGCAAGGTAGAGTTTAAAG
  CTCAGGACACCATTAGCAGGGGCTCAGATGACTCAGTGCCAGTTATTTCATTTAAAGATGCTGCTTTTGA
  TGATGTCAGTGGTACTGATGAAGGAAGACCTGATCTTCTTGTAAATTTACCTGGTGAATTGGAGTCAACA
  AGAGAAGCTGCAGCAATGGGACCTACTAAGTTTACACAAACTAATATAGGGATAATAGAAAATAAACTCT
  TGGAAGCCCCTGATGTTTTATGCCTCAGGCTTAGTACTGAACAATGCCAAGCACATGAGGAGAAAGGCAT
  AGAGGAACTGAGTGATCCCTCTGGGCCCAAATCCTATAGTATAACAGAGAAACACTATGCACAGGAGGAT
  CCCAGGATGTTATTTGTAGCAGCTGTTGATCATAGTAGTTCAGGAGATATGTCTTTGTTACCCAGCTCAG
  ATCCTAAGTTTCAAGGACTTGGAGTGGTTGAGTCAGCAGTAACTGCAAACAACACAGAAGAAAGCTTATT
  CCGTATTTGTAGTCCACTCTCAGGTGCTAATGAATATATTGCAAGCACAGACACTTTAAAAACAGAAGAA
  GTATTGCTGTTTACAGATCAGACTGATGATTTGGCTAAAGAGGAACCAACTTCTTTATTCCAGAGAGACT
  CTGAGACTAAGGGTGAAAGTGGTTTAGTGCTAGAAGGAGACAAGGAAATACATCAGATTTTTGAGGACCT
  TGATAAAAAATTAGCACTAGCCTCCAGGTTTTACATCCCAGAGGGCTGCATTCAAAGATGGGCAGCTGAA
  ATGGTGGTAGCCCTTGATGCTTTACATAGAGAGGGAATTGTGTGCCGCGATTTGAACCCAAACAACATCT
  TATTGAATGATAGAGGACACATTCAGCTAACGTATTTTAGCAGGTGGAGTGAGGTTGAAGATTCCTGTGA
  CAGCGATGCCATAGAGAGAATGTACTGTGCCCCAGAGGTTGGAGCAATCACTGAAGAAACTGAAGCCTGT
  GATTGGTGGAGTTTGGGTGCTGTCCTCTTTGAACTTCTCACTGGCAAGACTCTGGTTGAATGCCATCCAG
  CAGGAATAAATACTCACACTACTTTGAACATGCCAGAATGTGTCTCTGAAGAGGCTCGCTCACTCATTCA
  ACAGCTCTTGCAGTTCAATCCTCTGGAACGACTTGGTGCTGGAGTTGCTGGTGTTGAAGATATCAAATCT
  CATCCATTTTTTACCCCTGTGGATTGGGCAGAACTGATGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239688 representing NM_001287218
  Red=Cloning site Green=Tags(s)
  
  MASNQNSPIRTFGLNLSSDSSALGAVASDSEQSKTEEERESRSLFPGSLKPKLGKRDYLEKAGELIKLAL
  KKEEEDDYEAASDFYRKGVDLLLEGVQGESSPTRREAVKRRTAEYLMRAESISSLYGKPQLDDVSQVLLV
  MDTRTEQTFILKGLRKSSEYSRNRKTIIPRCVPNMVCLHKYIISEESVFLVLQHAEGGKLWSYISKFLNR
  SPEESFDIKEVKKPTLAKVHLQQPTSSPQDSSSFESRGSDGGSMLKALPLKSSLTPSSQDDSNQEDDGQD
  SSPKWPDSGSSSEEECTTSYLTLCNEYGQEKIEPGSLNEEPFMKTEGNGVDTKAIKSFPAHLAADSDSPS
  TQLRAHELKFFPNDDPEAVSSPRTSDSLSRSKNSPMEFFRIDSKDSASELLGLDFGEKLYSLKSEPLKPF
  FTLPDGDSASRSFNTSESKVEFKAQDTISRGSDDSVPVISFKDAAFDDVSGTDEGRPDLLVNLPGELEST
  REAAAMGPTKFTQTNIGIIENKLLEAPDVLCLRLSTEQCQAHEEKGIEELSDPSGPKSYSITEKHYAQED
  PRMLFVAAVDHSSSGDMSLLPSSDPKFQGLGVVESAVTANNTEESLFRICSPLSGANEYIASTDTLKTEE
  VLLFTDQTDDLAKEEPTSLFQRDSETKGESGLVLEGDKEIHQIFEDLDKKLALASRFYIPEGCIQRWAAE
  MVVALDALHREGIVCRDLNPNNILLNDRGHIQLTYFSRWSEVEDSCDSDAIERMYCAPEVGAITEETEAC
  DWWSLGAVLFELLTGKTLVECHPAGINTHTTLNMPECVSEEARSLIQQLLQFNPLERLGAGVAGVEDIKS
  HPFFTPVDWAELMR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001287218
• ORF Size: 2562 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001287218.2
• RefSeq Size: 4204 bp
• RefSeq ORF: 2565 bp
• Locus ID: 26750
• Cytogenetics: 1q32.3
• Protein Families: Druggable Genome, Protein Kinase
• MW: 94.9 kDa
• Gene Summary: Sphingosine kinase catalyzes the formation of sphingosine 1 phosphate, a lipid cellular messenger. The protein encoded by this gene can bind to sphingosine kinase and to phosphatidylinositol 3-phosphate, suggesting a role in sphingosine 1 phophate signaling. The encoded protein can also bind to peroxiredoxin-3 and may help transport it to mitochondria. [provided by RefSeq, Mar 2017]