SP140 (NM_001278451) Human Tagged ORF Clone

CAT#: RC239662

SP140 (myc-DDK-tagged) - Human SP140 nuclear body protein (SP140), transcript variant 3

Product Images

Circular map for RC239662

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SP140
• Synonyms: LYSP100; LYSP100-A; LYSP100-B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239662 representing NM_001278451
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGCAGGGCCAGCAGGGGCAGATGGCAAGTGGAGACAGCAATCTCAACTTCAGGATGGTCGCAG
  AGATCCAGAACGTAGAGGGTCAGAACCTGCAGGAGCAGGTTTGCCCTGAGCCCATTTTCAGGTTCTTCAG
  AGAAAACAAGGTGGAGATTGCAAGTGCAATAACAAGGCCATTTCCTTTCCTTATGGGCCTCCGAGACCGC
  TCCTTCATCTCCGAGCAGATGTATGAACATTTTCAAGAAGCTTTTAGAAACCTGGTCCCAGTGACAAGAG
  TGATGTATTGTGTACTCAGTGAACTGGAGAAGACATTTGGCTGGTCACATCTGGAAGCATTGTTCAGCAG
  GATTAACCTGATGGCCTATCCTGATTTAAACGAGATTTACAGAAGCTTCCAGAATGTATGCTATGAACAC
  TCACCTCTCCAAATGAATAATGTAAACGATTTAGAAGATAGACCCAGATTACTACCATATGGTAAACAAG
  AGAACAGCAATGCCTGTCATGAAATGGATGATATAGCAGTGCCTCAGGAAGCCTTGAGCTCCTCGCCAAG
  GTGTGAGCCAGGTTTCTCTTCAGAGTCTTGTGAGCAGTTAGCTCTCCCAAAGGCTGGTGGAGGAGATGCT
  GAAGATGCACCCAGCCTACTACCAGGTGGGGGAGTGTCCTGTAAACTTGCTATACAAATAGATGAAGGAG
  AATCAGAAGAAATGCCCAAGTTACTGCCTTATGATACAGAAGTTCTAGAAAGCAACGGGATGATAGATGC
  GGCAAGGACATACAGCACAGCACCAGGGGAGAAACAGGGAGAGGAGGAAGGCAGGAACAGTCCCAGAAAA
  AGAAACCAAGACAAGGAGAAGTACCAAGAGAGTCCAGAGGGAAGAGACAAAGAGACCTTTGATCTAAAAA
  CTCCCCAAGTCACTAATGAAGGAGAACCAGAGAAGGGGCTCTGTCTACTACCAGGTGAAGGAGAAGAGGG
  CAGTGATGACTGTTCAGAAATGTGTGATGGAGAAGAGCCCCAGGAAGCCTCTAGCTCCCTAGCAAGATGT
  GGGTCAGTTTCTTGTTTATCTGCAGAGACCTTTGATCTAAAGACTCCCCAAGTCACTAATGAAGGAGAAC
  CAGAGAAGGAGCTCAGTCTACTACCAGGTGAAGGAGAAGTGTCTAGTGAACTAGAAAATCACCCAATGAA
  TGAAGAAGGAGAATCAGAAGAGCTTGCTTCTAGCCTGCTATATGATAATGTACCAGGAGCGGAGCAATCA
  GCATATGAAAATGAGAAGTGTTCCTGTGTCATGTGTTTCTCAGAAGAGGTGCCAGGAAGCCCAGAAGCAA
  GGACGGAAAGTGATCAAGCGTGTGGCACAATGGATACTGTGGATATTGCAAACAACTCCACTTTGGGAAA
  ACCCAAGAGGAAAAGAAGAAAAAAGAGGGGGCATGGCTGGAGCAGAATGAGAATGAGAAGGCAGGAAAAC
  AGCCAACAAAATGATAATAGCAAAGCCGACGGCCAGGTGGTCTCCAGTGAAAAGAAGGCGAACGTGAATC
  TGAAAGACCTTTCCAAGATTAGGGGGAGAAAGAGAGGCAAACCTGGAACCCGCTTCACTCAGAGTGACAG
  AGCTGCACAGAAAAGAGTCCGATCAAGAGCTTCAAGAAAGCACAAAGATGAAACTGTGGATTTTAAGGCT
  CCTTTGCTTCCAGTGACCTGTGGTGGGGTGAAGGGAATTTTACATAAGAAGAAATTGCAGCAAGGAATCT
  TGGTGAAGTGTATACAGACTGAGGATGGAAAATGGTTCACCCCCACGGAATTTGAAATCAAAGGAGGCCA
  TGCAAGATCAAAGAACTGGAGGCTGAGTGTGCGCTGTGGCGGGTGGCCCCTACGATGGCTGATGGAGAAT
  GGATTTCTGCCTGATCCTCCAAGAATACGTTACAGGAAAAAAAAGAGAATACTGAAGTCTCAAAACAATA
  GCTCAGTTGACCCTTGTATGAGAAACCTGGATGAGTGTGAGGTGTGCCGGGACGGAGGGGAGCTGTTCTG
  TTGCGACACTTGTTCAAGAGTCTTCCATGAGGACTGTCACATCCCGCCTGTGGAAGCTGAGAGGACCCCG
  TGGAATTGCATCTTCTGCAGGATGAAGGAGTCTCCGGGAAGCCAACAGTGTTGTCAGGAATCTGAGGTCC
  TGGAGAGGCAGATGTGTCCTGAGGAACAGTTGAAATGTGAGTTCCTCCTCTTGAAAGTCTATTGCTGTTC
  TGAGAGCTCCTTTTTTGCCAAGATTCCATACTATTATTATATTAGAGAGGCGTGTCAAGGCCTGAAGGAG
  CCCATGTGGTTGGATAAAATCAAGAAAAGGCTGAATGAGCACGGTTACCCCCAAGTGGAGGGGTTTGTAC
  AAGACATGCGCCTCATCTTCCAGAACCACAGGGCCTCTTACAAGTACAAGGATTTTGGCCAAATGGGATT
  TAGACTGGAGGCTGAGTTTGAGAAGAATTTCAAGGAAGTGTTTGCTATTCAGGAAACAAATGGGAACAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239662 representing NM_001278451
  Red=Cloning site Green=Tags(s)
  
  MAQQGQQGQMASGDSNLNFRMVAEIQNVEGQNLQEQVCPEPIFRFFRENKVEIASAITRPFPFLMGLRDR
  SFISEQMYEHFQEAFRNLVPVTRVMYCVLSELEKTFGWSHLEALFSRINLMAYPDLNEIYRSFQNVCYEH
  SPLQMNNVNDLEDRPRLLPYGKQENSNACHEMDDIAVPQEALSSSPRCEPGFSSESCEQLALPKAGGGDA
  EDAPSLLPGGGVSCKLAIQIDEGESEEMPKLLPYDTEVLESNGMIDAARTYSTAPGEKQGEEEGRNSPRK
  RNQDKEKYQESPEGRDKETFDLKTPQVTNEGEPEKGLCLLPGEGEEGSDDCSEMCDGEEPQEASSSLARC
  GSVSCLSAETFDLKTPQVTNEGEPEKELSLLPGEGEVSSELENHPMNEEGESEELASSLLYDNVPGAEQS
  AYENEKCSCVMCFSEEVPGSPEARTESDQACGTMDTVDIANNSTLGKPKRKRRKKRGHGWSRMRMRRQEN
  SQQNDNSKADGQVVSSEKKANVNLKDLSKIRGRKRGKPGTRFTQSDRAAQKRVRSRASRKHKDETVDFKA
  PLLPVTCGGVKGILHKKKLQQGILVKCIQTEDGKWFTPTEFEIKGGHARSKNWRLSVRCGGWPLRWLMEN
  GFLPDPPRIRYRKKKRILKSQNNSSVDPCMRNLDECEVCRDGGELFCCDTCSRVFHEDCHIPPVEAERTP
  WNCIFCRMKESPGSQQCCQESEVLERQMCPEEQLKCEFLLLKVYCCSESSFFAKIPYYYYIREACQGLKE
  PMWLDKIKKRLNEHGYPQVEGFVQDMRLIFQNHRASYKYKDFGQMGFRLEAEFEKNFKEVFAIQETNGNN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278451
• ORF Size: 2520 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278451.1, NP_001265380.1
• RefSeq Size: 3189 bp
• RefSeq ORF: 2523 bp
• Locus ID: 11262
• UniProt ID: Q13342
• Cytogenetics: 2q37.1
• Protein Families: Druggable Genome, Transcription Factors
• MW: 95.8 kDa
• Gene Summary: This gene encodes a member of the SP100 family of proteins, which are share common domains including an N-terminal homogeneously staining region domain followed by a SP100/autoimmune regulator/NucP41/P75/deformed epidermal autoregulatory factor domain, a plant homeobox zinc finger, and a bromodomain. The encoded protein is interferon-inducible and is expressed at high levels in the nuclei of leukocytes. Variants of this gene have been associated with multiple sclerosis, Crohn's disease, and chronic lymphocytic leukemia. Alternative splicing results in multiple variants. [provided by RefSeq, Aug 2016]