SPCA2 (ATP2C2) (NM_001291454) Human Tagged ORF Clone

CAT#: RC239585

ATP2C2 (myc-DDK-tagged) - Human ATPase, Ca++ transporting, type 2C, member 2 (ATP2C2), transcript variant 3

Product Images

Circular map for RC239585

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SPCA2
• Synonyms: SPCA2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239585 representing NM_001291454
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGACGCCGTCAGCATCGCCACGGCAGTGCTTGTCGTGGTCACTGTCGCCTTCATCCAGCCTAAGAG
  AAGGAAAACTCCAGCACCTGCTTGCTCGAGAACTGGTTCCTGGTGATGTCGTATCTCTCTCGATCGGAGA
  CCGGATCCCTGCAGACATCCGACTCACTGAGGTCACGGACCTCTTGGTGGATGAATCCAGTTTCACCGGG
  GAAGCCGAGCCATGTAGTAAAACAGACAGCCCCTTGACAGGCGGTGGGGACCTCACCACCCTCAGCAACA
  TCGTCTTCATGGGGACCCTGGTGCAGTATGGGAGGGGCCAGGGGGTCGTGATTGGAACAGGGGAAAGCTC
  TCAGTTCGGAGAAGTGTTTAAGATGATGCAGGCTGAAGAGACACCTAAAACTCCTTTGCAGAAAAGCATG
  GACAGGCTAGGAAAGCAACTGACACTCTTCTCCTTTGGCATAATCGGTCTCATCATGCTCATTGGCTGGT
  CGCAAGGGAAACAACTCCTGAGTATGTTCACGATCGGGGTCAGCCTGGCTGTGGCGGCCATTCCAGAGGG
  TCTGCCCATCGTCGTCATGGTGACGCTGGTCCTGGGAGTGCTGCGGATGGCCAAGAAGCGGGTCATCGTG
  AAGAAGTTACCCATCGTGGAGACTTTAGGTTGCTGCAGCGTTCTCTGTTCTGACAAGACGGGGACTCTGA
  CTGCCAATGAAATGACAGTGACCCAGCTTGTAACGTCAGATGGGCTTCGTGCCGAGGTCAGCGGAGTTGG
  GTATGACGGTCAAGGGACTGTGTGTCTTCTACCATCCAAGGAAGTCATTAAGGAATTTTCCAATGTCTCA
  GTGGGAAAGTTAGTGGAGGCGGGCTGTGTTGCCAACAATGCGGTCATCAGAAAGAACGCCGTGATGGGGC
  AGCCCACCGAGGGTGCATTGATGGCCCTGGCGATGAAGATGGACTTAAGTGATATTAAAAATTCATATAT
  AAGAAAAAAAGAGATTCCATTCAGTTCAGAGCAGAAGTGGATGGCGGTGAAATGCAGTCTGAAGACTGAG
  GATCAGGAAGACATTTACTTCATGAAAGGGGCCTTGGAAGAGGTGATCCGCTACTGCACCATGTACAACA
  ACGGGGGCATCCCCCTGCCGCTGACGCCCCAGCAGAGGTCATTCTGCCTGCAGGAAGAGAAGAGGATGGG
  GTCGCTCGGTTTGCGGGTGCTGGCCCTGGCTTCTGGGCCCGAGCTGGGGCGGCTGACGTTTCTCGGTCTT
  GTGGGCATCATTGACCCCCCGAGAGTTGGCGTGAAGGAAGCAGTCCAGGTTCTCTCCGAGTCTGGTGTGT
  CTGTGAAGATGATAACGGGGGATGCCCTGGAGACGGCCTTGGCCATAGGAAGAAACATCGGCCTGTGCAA
  CGGGAAGCTGCAAGCCATGTCCGGGGAGGAGGTGGACAGCGTGGAGAAGGGCGAGCTGGCCGACCGCGTG
  GGGAAGGTGTCCGTGTTCTTCAGGACCAGCCCAAAGCACAAGCTCAAAATCATCAAGGCTCTGCAGGAGT
  CAGGGGCGATCGTGGCCATGACTGGGGATGGGGTGAACGACGCAGTGGCCCTGAAGTCTGCAGACATTGG
  GATCGCCATGGGGCAGACAGGGACGGACGTCAGCAAAGAGGCCGCCAACATGATCCTGGTGGATGATGAC
  TTCTCAGCCATCATGAATGCAGTGGAGGAAGGCAAGGGTATTTTTTACAACATCAAAAACTTTGTCCGAT
  TCCAGCTGAGCACGAGCATCTCCGCCCTGAGTCTCATCACTCTGTCCACCGTGTTCAACCTGCCCAGCCC
  CCTCAACGCCATGCAGATCCTATGGATCAACATCATCATGGATGGGCCACCGGCGCAGAGCTTGGGGGTA
  GAGCCCGTTGACAAAGACGCCTTCAGGCAGCCACCACGGAGTGTGCGGGACACCATCCTCAGCAGAGCCC
  TCATCCTGAAGATCCTCATGTCCGCGGCCATCATCATCAGCGGGACCCTCTTTATCTTCTGGAAGGAGAT
  GCCTGAAGACAGAGCAAGCACTCCCCGCACCACGACGATGACGTTCACTTGTTTTGTGTTTTTCGATCTC
  TTCAACGCCTTGACCTGCCGCTCTCAGACCAAGCTGATATTTGAGATCGGCTTTCTCAGGAACCACATGT
  TCCTCTACTCCGTCCTGGGGTCCATCCTGGGGCAGCTGGCGGTCATTTACATCCCCCCGCTGCAGAGGGT
  CTTCCAGACGGAGAACCTGGGAGCGCTTGATTTGCTGTTTTTAACTGGATTGGCCTCATCCGTCTTCATT
  TTGTCAGAGCTCCTCAAACTATGTGAAAAATACTGTTGCAGCCCCAAGAGAGTCCAGATGCACCCTGAAG
  ATGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239585 representing NM_001291454
  Red=Cloning site Green=Tags(s)
  
  MRTPSASPRQCLSWSLSPSSSLREGKLQHLLARELVPGDVVSLSIGDRIPADIRLTEVTDLLVDESSFTG
  EAEPCSKTDSPLTGGGDLTTLSNIVFMGTLVQYGRGQGVVIGTGESSQFGEVFKMMQAEETPKTPLQKSM
  DRLGKQLTLFSFGIIGLIMLIGWSQGKQLLSMFTIGVSLAVAAIPEGLPIVVMVTLVLGVLRMAKKRVIV
  KKLPIVETLGCCSVLCSDKTGTLTANEMTVTQLVTSDGLRAEVSGVGYDGQGTVCLLPSKEVIKEFSNVS
  VGKLVEAGCVANNAVIRKNAVMGQPTEGALMALAMKMDLSDIKNSYIRKKEIPFSSEQKWMAVKCSLKTE
  DQEDIYFMKGALEEVIRYCTMYNNGGIPLPLTPQQRSFCLQEEKRMGSLGLRVLALASGPELGRLTFLGL
  VGIIDPPRVGVKEAVQVLSESGVSVKMITGDALETALAIGRNIGLCNGKLQAMSGEEVDSVEKGELADRV
  GKVSVFFRTSPKHKLKIIKALQESGAIVAMTGDGVNDAVALKSADIGIAMGQTGTDVSKEAANMILVDDD
  FSAIMNAVEEGKGIFYNIKNFVRFQLSTSISALSLITLSTVFNLPSPLNAMQILWINIIMDGPPAQSLGV
  EPVDKDAFRQPPRSVRDTILSRALILKILMSAAIIISGTLFIFWKEMPEDRASTPRTTTMTFTCFVFFDL
  FNALTCRSQTKLIFEIGFLRNHMFLYSVLGSILGQLAVIYIPPLQRVFQTENLGALDLLFLTGLASSVFI
  LSELLKLCEKYCCSPKRVQMHPEDV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291454
• ORF Size: 2385 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291454.1, NP_001278383.1
• RefSeq Size: 2987 bp
• RefSeq ORF: 2388 bp
• Locus ID: 9914
• UniProt ID: O75185
• Cytogenetics: 16q24.1
• Protein Families: Druggable Genome, Transmembrane
• MW: 86.6 kDa
• Gene Summary: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium.[UniProtKB/Swiss-Prot Function]