ZNF43 (NM_001256654) Human Tagged ORF Clone

CAT#: RC239458

ZNF43 (myc-DDK-tagged) - Human zinc finger protein 43 (ZNF43), transcript variant 7

Product Images

Circular map for RC239458

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF43
• Synonyms: HTF6; KOX27; ZNF39L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239458 representing NM_001256654
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAGACATGAAATGGTAGCCAAACCCCCAGTTATGTGTTCTCATTTTACCCAAGACTTTTGGCCAG
  AGCAGCATATAAAAGATCCTTTCCAAAAAGCGACACTGAGAAGATATAAAAACTGTGAACATAAAAATGT
  ACATTTAAAAAAAGACCATAAAAGTGTGGATGAGTGTAAGGTGCACAGAGGAGGTTATAATGGATTTAAC
  CAATGTTTGCCAGCTACCCAGAGCAAAATATTTCTATTTGATAAATGTGTGAAAGCCTTTCATAAATTTT
  CAAATTCAAACAGACATAAGATAAGCCATACTGAAAAAAAACTTTTCAAATGCAAAGAATGTGGCAAATC
  ATTTTGCATGCTTCCACATCTAGCTCAACATAAAATAATTCATACCAGAGTGAATTTCTGCAAATGTGAA
  AAATGTGGAAAAGCTTTTAACTGCCCTTCAATCATCACTAAACATAAGAGAATTAATACTGGAGAGAAAC
  CCTACACATGTGAAGAATGTGGCAAAGTCTTTAATTGGTCCTCACGCCTTACTACACATAAAAAAAATTA
  TACTAGATACAAACTCTACAAATGTGAAGAATGTGGCAAAGCTTTTAACAAGTCCTCAATCCTTACTACC
  CATAAGATAATTCGCACTGGAGAGAAATTCTACAAATGTAAAGAATGTGCCAAAGCTTTTAACCAATCCT
  CAAACCTTACTGAACATAAGAAAATTCATCCTGGAGAGAAACCTTACAAATGTGAAGAATGTGGCAAAGC
  CTTTAACTGGCCCTCAACTCTTACTAAACATAAGAGAATTCATACTGGAGAGAAACCCTACACATGTGAA
  GAATGTGGCAAAGCCTTTAACCAGTTCTCAAACCTTACTACACATAAGAGAATCCATACTGCAGAGAAAT
  TCTATAAATGTACAGAATGTGGTGAAGCTTTTAGCCGGTCCTCAAACCTTACTAAACATAAGAAAATTCA
  TACTGAAAAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCTTTTAAGTGGTCCTCAAAGCTTACTGAA
  CATAAGTTAACTCATACTGGAGAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCCTTTAACTGGCCCT
  CAACCCTTACTAAACATAACAGAATTCATACTGGAGAGAAACCCTACAAATGTGAAGTATGTGGCAAAGC
  CTTTAACCAGTTCTCAAACCTTACTACACATAAGAGAATTCATACTGCAGAAAAACCGTACAAATGTGAA
  GAATGTGGCAAAGCTTTTAGCCGGTCCTCAAACCTTACTAAACATAAGAAAATTCACATTGAAAAGAAAC
  CCTACAAATGTGAAGAATGTGGCAAAGCTTTTAAGTGGTCCTCAAAGCTTACTGAACATAAGATAACTCA
  TACTGGAGAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCTTTTAACCATTTCTCAATCCTTACCAAA
  CATAAGAGGATTCATACTGGAGAGAAACCCTACAAGTGTGAAGAATGTGGCAAAGCTTTTACCCAATCCT
  CAAACCTTACTACACATAAGAAAATTCATACTGGAGAGAAATTCTACAAATGTGAAGAATGTGGCAAAGC
  TTTTACCCAATCTTCAAACCTTACTACACATAAAAAAATTCATACTGGAGGAAAACCCTACAAATGTGAA
  GAATGTGGCAAAGCTTTTAACCAGTTCTCAACTCTTACTAAACATAAGATAATTCACACTGAGGAGAAAC
  CCTACAAATGTGAAGAATGTGGCAAAGCCTTTAAGTGGTCCTCAACCCTTACTAAACATAAGATAATTCA
  TACTGGAGAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCTTTTAAACTGTCCTCAACCCTTTCTACA
  CATAAGATTATTCATACTGGAGAGAAACCCTACAAATGTGAAAAATGTGGCAAAGCTTTTAACCGATCCT
  CAAACCTTATTGAACATAAGAAAATTCATACTGGAGAGCAACCCTACAAATGTGAAGAATGTGGCAAAGC
  ATTTAACTATTCCTCACACCTTAATACACATAAGAGAATTCATACTAAAGAGCAACCCTACAAATGTAAA
  GAATGTGGCAAAGCTTTCAACCAATATTCAAACCTTACTACACATAACAAAATTCATACTGGAGAGAAAC
  TCTACAAACCTGAAGATGTGACAGTGATTTTGACAACACCTCAAACTTTTTCAAACATAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239458 representing NM_001256654
  Red=Cloning site Green=Tags(s)
  
  MRRHEMVAKPPVMCSHFTQDFWPEQHIKDPFQKATLRRYKNCEHKNVHLKKDHKSVDECKVHRGGYNGFN
  QCLPATQSKIFLFDKCVKAFHKFSNSNRHKISHTEKKLFKCKECGKSFCMLPHLAQHKIIHTRVNFCKCE
  KCGKAFNCPSIITKHKRINTGEKPYTCEECGKVFNWSSRLTTHKKNYTRYKLYKCEECGKAFNKSSILTT
  HKIIRTGEKFYKCKECAKAFNQSSNLTEHKKIHPGEKPYKCEECGKAFNWPSTLTKHKRIHTGEKPYTCE
  ECGKAFNQFSNLTTHKRIHTAEKFYKCTECGEAFSRSSNLTKHKKIHTEKKPYKCEECGKAFKWSSKLTE
  HKLTHTGEKPYKCEECGKAFNWPSTLTKHNRIHTGEKPYKCEVCGKAFNQFSNLTTHKRIHTAEKPYKCE
  ECGKAFSRSSNLTKHKKIHIEKKPYKCEECGKAFKWSSKLTEHKITHTGEKPYKCEECGKAFNHFSILTK
  HKRIHTGEKPYKCEECGKAFTQSSNLTTHKKIHTGEKFYKCEECGKAFTQSSNLTTHKKIHTGGKPYKCE
  ECGKAFNQFSTLTKHKIIHTEEKPYKCEECGKAFKWSSTLTKHKIIHTGEKPYKCEECGKAFKLSSTLST
  HKIIHTGEKPYKCEKCGKAFNRSSNLIEHKKIHTGEQPYKCEECGKAFNYSSHLNTHKRIHTKEQPYKCK
  ECGKAFNQYSNLTTHNKIHTGEKLYKPEDVTVILTTPQTFSNIK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256654
• ORF Size: 2232 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256654.2
• RefSeq Size: 5131 bp
• RefSeq ORF: 2235 bp
• Locus ID: 7594
• Cytogenetics: 19p12
• Protein Families: Transcription Factors
• MW: 86.9 kDa
• Gene Summary: This gene belongs to the C2H2-type zinc finger gene family. The zinc finger proteins are involved in gene regulation and development, and are quite conserved throughout evolution. Like this gene product, a third of the zinc finger proteins containing C2H2 fingers also contain the KRAB domain, which has been found to be involved in protein-protein interactions. [provided by RefSeq, Jul 2008]