RNF98 (TRIM36) (NM_001300759) Human Tagged ORF Clone
CAT#: RC239393
- TrueORF®
TRIM36 (myc-DDK-tagged) - Human tripartite motif containing 36 (TRIM36), transcript variant 5
ORF Plasmid: tGFP
"NM_001300759" in other vectors (2)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | RNF98 |
Synonyms | ANPH; HAPRIN; RBCC728; RNF98 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC239393 representing NM_001300759
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGCGATGGCTCAGATTCGCCGGTTACCATTAAGAATATCGAAAGGGAGCTCATTTGCCCAGCAT GCAAGGAGCTGTTTACCCACCCATTGATTCTCCCTTGCCAACATAGTATCTGTCATAAATGTGTAAAAGA ACTCCTGCTGACTCTCGATGATTCATTCAACGATGTGGGATCAGACAACTCCAATCAAAGCAGTCCTCGA CTTCGGCTCCCCTCCCCTAGTATGGATAAAATTGACCGAATTAACAGACCAGGCTGGAAGCGCAATTCAT TGACCCCGAGGACAACTGTTTTCCCTTGCCCTGGCTGTGAGCATGATGTGGATCTTGGAGAACGAGGAAT CAATGGTCTGTTTCGAAACTTCACTTTGGAAACTATTGTGGAAAGATATCGTCAAGCAGCTAGGGCAGCC ACAGCCATTATGTGTGACCTTTGTAAACCACCACCTCAAGAATCCACAAAAAGCTGCATGGACTGTAGTG CAAGTTACTGCAATGAATGCTTCAAAATTCATCACCCTTGGGGTACTATAAAAGCTCAACATGAGTATGT TGGTCCAACTACTAACTTCAGACCCAAGATTTTAATGTGCCCAGAACATGAAACAGAGAGAATAAACATG TACTGTGAATTATGTAGGAGGCCAGTTTGCCATCTGTGTAAGTTGGGTGGTAATCATGCCAACCACCGTG TAACCACTATGAGCAGTGCCTACAAAACCTTAAAGGAAAAGCTTTCAAAGGATATTGATTACCTTATTGG TAAGGAAAGCCAGGTGAAGAGTCAAATATCTGAACTAAACTTGTTAATGAAAGAAACAGAGTGTAATGGA GAGAGGGCTAAAGAAGAAGCAATTACACATTTTGAAAAGCTCTTTGAAGTTCTGGAAGAGAGGAAATCAT CTGTTTTGAAAGCAATTGACTCCTCTAAGAAACTAAGATTAGACAAATTTCAGACTCAAATGGAAGAGTA CCAGGGACTTCTAGAGAACAATGGACTTGTGGGATATGCTCAAGAAGTGCTAAAGGAGACAGATCAGTCT TGCTTTGTGCAGACAGCAAAGCAGCTCCACCTCAGAATACAGAAAGCCACAGAATCTTTGAAGAGCTTTA GACCTGCAGCTCAGACTTCTTTTGAAGACTATGTTGTTAATACCTCTAAACAAACAGAACTTCTTGGAGA ATTATCCTTTTTCTCTAGTGGCATAGACGTGCCAGAGATCAATGAGGAACAGAGCAAAGTTTATAACAAT GCCTTGATAAATTGGCACCATCCAGAAAAGGATAAAGCTGATAGCTATGTTCTTGAATATCGGAAAATCA ATAGAGATGATGAAATGTCATGGAATGAGATAGAAGTGTGTGGAACAAGTAAAATAATTCAAGACTTGGA AAACAGTAGTACCTATGCTTTCAGAGTAAGAGCTTACAAGGGTTCAATCTGTAGTCCTTGCAGCAGAGAA TTGATTCTTCATACTCCTCCAGCTCCAGTTTTCAGCTTCCTCTTTGATGAAAAATGTGGCTATAATAATG AACACCTCCTGCTGAACTTGAAGAGAGACCGTGTAGAGAGTAGAGCTGGATTTAATCTTCTGCTTGCTGC AGAACGCATCCAAGTGGGTTATTACACAAGCTTAGACTACATCATTGGAGATACTGGCATTACAAAAGGA AAACACTTCTGGGCCTTCCGTGTGGAACCATATTCATACCTGGTAAAAGTGGGAGTTGCTTCTAGCGATA AACTACAAGAATGGCTCCGTTCTCCCCGGGATGCAGTTAGTCCAAGATATGAGCAAGACAGTGGGCATGA CAGTGGAAGTGAGGATGCCTGTTTTGATTCTTCACAACCATTTACCTTAGTTACTATAGGCATGCAGAAA TTTTTTATACCCAAGTCACCTACTTCTTCTAATGAACCTGAAAATAGAGTTCTCCCTATGCCAACAAGTA TTGGGATTTTCCTTGACTGTGATAAAGGCAAAGTAGATTTCTATGATATGGATCAGATGAAATGCCTTTA TGAACGCCAAGTGGACTGTTCACATACACTGTATCCAGCATTTGCATTAATGGGCAGTGGAGGAATTCAG CTTGAAGAACCCATCACAGCAAAATATCTGGAATACCAAGAGGACATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC239393 representing NM_001300759
Red=Cloning site Green=Tags(s) MEGDGSDSPVTIKNIERELICPACKELFTHPLILPCQHSICHKCVKELLLTLDDSFNDVGSDNSNQSSPR LRLPSPSMDKIDRINRPGWKRNSLTPRTTVFPCPGCEHDVDLGERGINGLFRNFTLETIVERYRQAARAA TAIMCDLCKPPPQESTKSCMDCSASYCNECFKIHHPWGTIKAQHEYVGPTTNFRPKILMCPEHETERINM YCELCRRPVCHLCKLGGNHANHRVTTMSSAYKTLKEKLSKDIDYLIGKESQVKSQISELNLLMKETECNG ERAKEEAITHFEKLFEVLEERKSSVLKAIDSSKKLRLDKFQTQMEEYQGLLENNGLVGYAQEVLKETDQS CFVQTAKQLHLRIQKATESLKSFRPAAQTSFEDYVVNTSKQTELLGELSFFSSGIDVPEINEEQSKVYNN ALINWHHPEKDKADSYVLEYRKINRDDEMSWNEIEVCGTSKIIQDLENSSTYAFRVRAYKGSICSPCSRE LILHTPPAPVFSFLFDEKCGYNNEHLLLNLKRDRVESRAGFNLLLAAERIQVGYYTSLDYIIGDTGITKG KHFWAFRVEPYSYLVKVGVASSDKLQEWLRSPRDAVSPRYEQDSGHDSGSEDACFDSSQPFTLVTIGMQK FFIPKSPTSSNEPENRVLPMPTSIGIFLDCDKGKVDFYDMDQMKCLYERQVDCSHTLYPAFALMGSGGIQ LEEPITAKYLEYQEDM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001300759 |
ORF Size | 2148 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001300759.2 |
RefSeq Size | 4240 bp |
RefSeq ORF | 2151 bp |
Locus ID | 55521 |
UniProt ID | Q9NQ86 |
Cytogenetics | 5q22.3 |
Protein Families | Druggable Genome |
MW | 82 kDa |
Gene Summary | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
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