CRNKL1 (NM_001278628) Human Tagged ORF Clone

CAT#: RC239306

CRNKL1 (myc-DDK-tagged) - Human crooked neck pre-mRNA splicing factor 1 (CRNKL1), transcript variant 5

Product Images

Circular map for RC239306

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CRNKL1
• Synonyms: CLF; Clf1; CRN; HCRN; MSTP021; SYF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239306 representing NM_001278628
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCTCCACCGCGGCCGGGAAGCAGCGGATTCCCAAAGTGGCCAAGGTGAAAAACAAAGCCCCGG
  CTGAGGTACAGATAACTGCTGAACAACTCTTAAGAGAGGCTAAAGAAAGAGAACTTGAGCTTCTTCCACC
  TCCACCTCAACAGAAGATCACAGATGAAGAAGAATTAAATGATTATAAACTAAGGAAAAGGAAGACTTTT
  GAAGATAATATAAGAAAAAACAGGACTGTGATTAGTAACTGGATAAAATACGCACAATGGGAAGAAAGCC
  TAAAGGAGATTCAAAGGGCTCGATCCATATACGAGCGTGCTTTAGATGTAGACTACCGAAATATTACACT
  CTGGCTGAAATACGCAGAAATGGAAATGAAGAATCGCCAAGTCAACCATGCTCGAAATATCTGGGACCGG
  GCCATAACAACGCTGCCTCGAGTTAATCAGTTCTGGTACAAGTACACGTACATGGAGGAAATGTTGGGAA
  ACGTTGCCGGTGCCCGGCAGGTGTTTGAGCGCTGGATGGAGTGGCAGCCTGAGGAGCAAGCCTGGCACTC
  CTACATCAACTTTGAGCTGAGATACAAAGAGGTGGATCGGGCCCGCACCATTTATGAGCGATTTGTCCTC
  GTGCACCCTGATGTTAAGAACTGGATCAAGTATGCCCGCTTTGAAGAAAAACATGCTTATTTTGCCCATG
  CACGGAAAGTGTATGAGAGAGCTGTGGAATTCTTTGGAGATGAACATATGGATGAGCACCTTTATGTTGC
  CTTTGCCAAGTTTGAAGAAAATCAGAAAGAGTTTGAAAGGGTACGAGTGATTTACAAGTATGCCCTGGAC
  AGAATTTCAAAACAAGATGCCCAAGAACTCTTTAAAAATTATACCATCTTTGAGAAGAAGTTTGGTGATA
  GGCGGGGTATTGAAGATATCATTGTGAGCAAACGGAGATTCCAGTACGAAGAAGAAGTGAAGGCGAATCC
  ACACAATTATGATGCATGGTTTGATTACTTGCGCTTGGTAGAAAGTGACGCAGAAGCTGAAGCCGTGAGA
  GAAGTCTATGAAAGGGCCATTGCCAATGTCCCACCCATTCAGGAGAAGAGGCACTGGAAGCGCTACATTT
  ATCTTTGGATCAACTATGCACTCTATGAAGAATTGGAGGCAAAGGATCCTGAGAGGACAAGACAGGTGTA
  TCAAGCCTCTTTGGAACTAATTCCTCACAAAAAGTTCACATTTGCCAAAATGTGGATACTGTATGCACAG
  TTTGAAATACGACAGAAGAATCTGTCATTAGCCAGAAGAGCATTGGGAACTTCCATAGGCAAATGTCCAA
  AGAACAAATTATTTAAAGTTTACATAGAATTGGAGCTACAGCTTCGAGAATTTGACAGATGCCGGAAGCT
  TTATGAAAAGTTCCTGGAATTTGGACCTGAAAATTGTACCTCATGGATTAAATTCGCTGAATTAGAGACA
  ATCCTTGGTGATATTGACAGAGCACGGGCAATCTATGAATTAGCCATCAGTCAGCCACGTTTAGACATGC
  CAGAGGTGCTTTGGAAATCATATATTGATTTTGAAATTGAGCAGGAAGAAACAGAAAGAACACGAAACCT
  TTACCGGCGGTTGCTTCAACGGACGCAGCATGTCAAGGTATGGATCAGCTTTGCTCAGTTTGAGTTGTCT
  TCAGGAAAAGAAGGAAGTTTGACTAAATGCAGACAAATTTATGAAGAAGCTAACAAAACCATGCGAAACT
  GTGAAGAAAAGGAAGAGAGACTTATGCTGCTGGAATCTTGGCGAAGTTTTGAAGAAGAATTTGGAACAGC
  TTCAGATAAGGAGAGAGTAGACAAACTCATGCCAGAGAAAGTCAAGAAGAGAAGAAAGGTCCAGACTGAT
  GATGGGTCTGATGCAGGCTGGGAAGAATACTTTGATTACATCTTTCCAGAAGATGCTGCCAACCAACCTA
  ACCTCAAACTCCTGGCCATGGCCAAACTGTGGAAGAAACAGCAGCAGGAAAAGGAGGATGCTGAGCACCA
  TCCAGATGAGGACGTCGATGAGAGTGAATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239306 representing NM_001278628
  Red=Cloning site Green=Tags(s)
  
  MAASTAAGKQRIPKVAKVKNKAPAEVQITAEQLLREAKERELELLPPPPQQKITDEEELNDYKLRKRKTF
  EDNIRKNRTVISNWIKYAQWEESLKEIQRARSIYERALDVDYRNITLWLKYAEMEMKNRQVNHARNIWDR
  AITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYINFELRYKEVDRARTIYERFVL
  VHPDVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHMDEHLYVAFAKFEENQKEFERVRVIYKYALD
  RISKQDAQELFKNYTIFEKKFGDRRGIEDIIVSKRRFQYEEEVKANPHNYDAWFDYLRLVESDAEAEAVR
  EVYERAIANVPPIQEKRHWKRYIYLWINYALYEELEAKDPERTRQVYQASLELIPHKKFTFAKMWILYAQ
  FEIRQKNLSLARRALGTSIGKCPKNKLFKVYIELELQLREFDRCRKLYEKFLEFGPENCTSWIKFAELET
  ILGDIDRARAIYELAISQPRLDMPEVLWKSYIDFEIEQEETERTRNLYRRLLQRTQHVKVWISFAQFELS
  SGKEGSLTKCRQIYEEANKTMRNCEEKEERLMLLESWRSFEEEFGTASDKERVDKLMPEKVKKRRKVQTD
  DGSDAGWEEYFDYIFPEDAANQPNLKLLAMAKLWKKQQQEKEDAEHHPDEDVDESES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278628
• ORF Size: 2061 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278628.2
• RefSeq Size: 4048 bp
• RefSeq ORF: 2064 bp
• Locus ID: 51340
• UniProt ID: Q9BZJ0
• Cytogenetics: 20p11.23
• Protein Pathways: Spliceosome
• MW: 83.6 kDa
• Gene Summary: The crooked neck (crn) gene of Drosophila is essential for embryogenesis and is thought to be involved in cell cycle progression and pre-mRNA splicing. A protein encoded by this human locus has been found to localize to pre-mRNA splicing complexes in the nucleus and is necessary for pre-mRNA splicing. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2013]