C6orf70 (ERMARD) (NM_001278531) Human Tagged ORF Clone

CAT#: RC239150

ERMARD (myc-DDK-tagged) - Human ER membrane-associated RNA degradation (ERMARD), transcript variant 2

Product Images

Circular map for RC239150

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C6orf70
• Synonyms: C6orf70; dJ266L20.3; PVNH6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239150 representing NM_001278531
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTATTAATAGGGGACCCTATTACCACATGTCTTTCTCCCTCAGTGTATGATATAATTTGTAATC
  TTGGGTTTCAACTCAGAGAAAATTGTGATATCAATAGCATTGTAACTCAGAATGGTGAAGTATGCTGGAA
  AACAATCACAGACTGTGTGAGCTACACAGAGTCAGAGCAGGGTCTGGATTACTGGGGAAGCGTGAGGCTG
  CTGGGCCCTGTGTGTGAGGCTGTCCATTCACATTTCTTATCTCTGACCAAGGGGCAATTTGAAATTCGAT
  ATGCACCGTGGTTCCAGTGGACAAGTTTTCCAGAGTTATTTCCTGAAATATTTGATGCCTTGGAAAGTCT
  ACAATCTCCTGCTATTTCTCTTAGCTTAATGAAACTGACATCGTGTCTAGAACGAGCCTTGGGTGATGTA
  TTTTTACTGATTGGGAAGGAATGCCCCTTTCTTTTAAGAGATCTGCTTTCATCTGAGGAGCTTGCTCAAG
  TCTTCAGTCAGTCTGTGATGAATGTGCTAAAAGTCTTCGTTGGCTCTCCGTGTGGTCTCAACCTGCGTAA
  CGTCTTATGGCATGGGTTTGCGTCACCTGAAGAAATTCCTCCAAAATACTGTTCAATGATGATACTGTTG
  ACGGCAGGATTGGGTCAGTTACTGAAGAGTTACCTTCAAAACACTAAACTTACATTGGCACATCGCTCTT
  TCATATCTCTTACAAACCTCGAGGATTTGATTGTTTTTCCTGATGTTACTTATGAGGTGCTTTCAGTATT
  AGAAGAAGTGATGATGAAATCTGCTTTTATATTAAAAATCATGTTACCATATTGGGAAGTTGCACTGGTC
  AAGTTCAAGTCACACAGGTTTGCTGACTGCGCCATATTGTTGCTGACACAACTGGAGACTGGACTTAGGA
  ATGTTTTTGCCACACTTAACAGATGTCCAAAAAGACTCCTGACTGCTGAGTCAACAGCTCTTTATACCAC
  CTTTGATCAAATATTGGCAAAACACTTGAATGATGGTAAAATCAATCAGCTTCCTCTTTTCCTTGGAGAG
  CCTGCTATGGAATTTCTCTGGGATTTCCTGAACCATCAGGAGGGTCCCCGCATAAGAGATCATTTAAGCC
  ACGGGGAGATCAACTTACATGAATTTTCAAAAGAAACAACTAATCAGTTGCTTGCATTTTCTCTTGTACT
  GCTACTCAGATTCGTTGATGACTGTCTGCTATCAGTTTTTAAGGAAAAATCAGCCGTAGAATTGTTGATT
  AGTCTTGCAGAAGGCTATAGTTCTCGCTGTCATCCGGTTTTTCAGCTTAAAAAACAGGTGCTGAGCTGTG
  AGGAGAGCATCAGGGTTTGGGCTCTGCTGCCTTTCCCCGAAGAACTCACTCGGCAAGCCGTCAGATTAGA
  AGATAATTCTGAAACAAATGCCTGCCACTCTTTGATTACAAAAATGACGGATGAGCTGTATCACCATATG
  CCTGAGAATCGTTGTGTGTTAAAGGACTTGGATCGTCTTCCTACTGAGACACCAACCAGCGATACGCCGC
  TAGCCCTGGCTCCCAGAAAGCCGCAGCCTTGCAGGTGCAGAAGGAGAGTGAGGCCCAGTATCAGACTACT
  GTCCCCTGTGCTCAGCCTGATACTGTTACTCATTGCGCTGGAGTTGGTCAACATTCATGCTGTTTGTGGG
  AAGAATGCGCATGAGTATCAGCAGTACCTAAAGTTTGTAAAGTCGATCTTGCAGTACACGGAGAACCTGG
  TGGCTTACACCAGTTACGAAAAGAACAAGTGGAATGAAACTATCAATCTTACACATACAGCTTTGTTGAA
  AATGTGGACTTTTAGTGAGAAGAAACAAATGTTAATACATTTAGCCAAGAAATCCACAAGTAAAGTACTC
  TTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239150 representing NM_001278531
  Red=Cloning site Green=Tags(s)
  
  MEVLIGDPITTCLSPSVYDIICNLGFQLRENCDINSIVTQNGEVCWKTITDCVSYTESEQGLDYWGSVRL
  LGPVCEAVHSHFLSLTKGQFEIRYAPWFQWTSFPELFPEIFDALESLQSPAISLSLMKLTSCLERALGDV
  FLLIGKECPFLLRDLLSSEELAQVFSQSVMNVLKVFVGSPCGLNLRNVLWHGFASPEEIPPKYCSMMILL
  TAGLGQLLKSYLQNTKLTLAHRSFISLTNLEDLIVFPDVTYEVLSVLEEVMMKSAFILKIMLPYWEVALV
  KFKSHRFADCAILLLTQLETGLRNVFATLNRCPKRLLTAESTALYTTFDQILAKHLNDGKINQLPLFLGE
  PAMEFLWDFLNHQEGPRIRDHLSHGEINLHEFSKETTNQLLAFSLVLLLRFVDDCLLSVFKEKSAVELLI
  SLAEGYSSRCHPVFQLKKQVLSCEESIRVWALLPFPEELTRQAVRLEDNSETNACHSLITKMTDELYHHM
  PENRCVLKDLDRLPTETPTSDTPLALAPRKPQPCRCRRRVRPSIRLLSPVLSLILLLIALELVNIHAVCG
  KNAHEYQQYLKFVKSILQYTENLVAYTSYEKNKWNETINLTHTALLKMWTFSEKKQMLIHLAKKSTSKVL
  L
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278531
• ORF Size: 1893 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278531.2
• RefSeq Size: 2003 bp
• RefSeq ORF: 1896 bp
• Locus ID: 55780
• UniProt ID: Q5T6L9
• Cytogenetics: 6q27
• Protein Families: Transmembrane
• MW: 72.4 kDa
• Gene Summary: The protein encoded by this gene contains 2 transmembrane domains near the C-terminus and is localized in the endoplasmic reticulum. Knockout of this gene in developing rat brain showed that it may be involved in neuronal migration. Mutations in this gene are associated with periventricular nodular heterotopia-6 (PVNH6). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]