VPS33B (NM_001289148) Human Tagged ORF Clone

CAT#: RC239011

VPS33B (myc-DDK-tagged) - Human vacuolar protein sorting 33 homolog B (yeast) (VPS33B), transcript variant 2

Product Images

Circular map for RC239011

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VPS33B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239011 representing NM_001289148
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTTCCCCATCGGCCGGACGCCCCTGAGCTGCCTGACTTCTCCATGCTGAAGAGGCTGGCTCGAG
  ACCAGCTCATCTATCTGCTGGAGCAGCAACACGAAGTAGACAAGCTATACAAGGTGGAGAACAAGCCAGC
  CCTCAGCTCCAATGAACAATTGTGCTTCTTGGTCAGACCCCGCATCAAGAATATGCGATACATTGCCAGT
  CTTGTCAATGCTGACAAATTGGCTGGCCGAACTCGCAAATACAAAGTGATCTTCAGCCCTCAAAAGTTCT
  ATGCGTGTGAGATGGTGCTTGAGGAAGAGGGAATCTATGGAGATGTGAGCTGTGATGAATGGGCCTTCTC
  TTTGCTGCCTCTTGATGTGGATCTGCTGAGCATGGAACTACCAGAATTTTTCAGGGATTACTTTCTGGAA
  GGAGATCAGCGTTGGATCAACACTGTAGCTCAGGCCTTACACCTTCTCAGCACTCTCTATGGACCCTTTC
  CAAACTGCTATGGAATTGGCAGGTGCGCCAAGATGGCATATGAATTGTGGAGGAACCTGGAGGAGGAGGA
  GGATGGCGAAACCAAGGGCCGAAGGCCAGAGATTGGACATATCTTTCTCTTGGACAGAGATGTGGACTTT
  GTGACAGCACTTTGCTCCCAAGTGGTTTATGAGGGCCTAGTAGATGACACCTTCCGCATCAAGTGTGGGA
  GTGTCGACTTTGGCCCAGAAGTCACATCCTCTGACAAGAGCCTGAAGGTGCTACTCAATGCCGAGGACAA
  GGTGTTTAATGAGATTCGGAACGAGCACTTCTCCAATGTCTTTGGCTTCTTGAGCCAGAAGGCCCGGAAC
  TTGCAGGCCCAGTATGATCGCCGGAGAGGCATGGACATTAAGCAGATGAAGAATTTCGTGTCCCAGGAGC
  TCAAGGGCCTGAAACAGGAGCACCGCCTGCTGAGTCTCCATATTGGGGCCTGTGAATCCATCATGAAGAA
  GAAAACCAAGCAGGATTTCCAGGAGCTAATCAAGACTGAGCATGCACTGCTAGAGGGGTTCAACATCCGG
  GAGAGCACCAGCTACATTGAGGAACACATAGACCGGCAGGTGTCGCCTATAGAAAGCCTGCGCCTCATGT
  GCCTTTTGTCCATCACTGAGAATGGTTTGATCCCCAAGGATTACCGATCTCTGAAAACACAGTATCTGCA
  GAGCTATGGCCCTGAGCACCTGCTAACCTTCTCCAATCTGCGAAGAGCTGGGCTCCTAACGGAGCAGGCC
  CCCGGGGACACCCTCACAGCCGTGGAGAGTAAAGTGAGCAAGCTGGTGACCGACAAGGCTGCAGGAAAGA
  TTACTGATGCCTTCAGTTCTCTGGCCAAGAGGAGCAATTTTCGTGCCATCAGCAAAAAGCTGAATTTGAT
  CCCACGTGTGGACGGCGAGTATGATCTGAAAGTGCCCCGAGACATGGCTTACGTCTTCGGTGGTGCTTAT
  GTGCCCCTGAGCTGCCGAATCATTGAGCAGGTGCTAGAGCGGCGAAGCTGGCAGGGCCTTGATGAGGTGG
  TACGGCTGCTCAACTGCAGTGACTTTGCATTCACAGATATGACTAAGGAAGACAAGGCTTCCAGTGAGTC
  CCTGCGCCTCATCTTGGTGGTGTTCTTGGGTGGTTGTACATTCTCTGAGATCTCAGCCCTCCGGTTCCTG
  GGCAGAGAGAAAGGCTACAGGTTCATTTTCCTGACGACAGCAGTCACAAACAGCGCTCGCCTTATGGAGG
  CCATGAGTGAGGTGAAAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239011 representing NM_001289148
  Red=Cloning site Green=Tags(s)
  
  MAFPHRPDAPELPDFSMLKRLARDQLIYLLEQQHEVDKLYKVENKPALSSNEQLCFLVRPRIKNMRYIAS
  LVNADKLAGRTRKYKVIFSPQKFYACEMVLEEEGIYGDVSCDEWAFSLLPLDVDLLSMELPEFFRDYFLE
  GDQRWINTVAQALHLLSTLYGPFPNCYGIGRCAKMAYELWRNLEEEEDGETKGRRPEIGHIFLLDRDVDF
  VTALCSQVVYEGLVDDTFRIKCGSVDFGPEVTSSDKSLKVLLNAEDKVFNEIRNEHFSNVFGFLSQKARN
  LQAQYDRRRGMDIKQMKNFVSQELKGLKQEHRLLSLHIGACESIMKKKTKQDFQELIKTEHALLEGFNIR
  ESTSYIEEHIDRQVSPIESLRLMCLLSITENGLIPKDYRSLKTQYLQSYGPEHLLTFSNLRRAGLLTEQA
  PGDTLTAVESKVSKLVTDKAAGKITDAFSSLAKRSNFRAISKKLNLIPRVDGEYDLKVPRDMAYVFGGAY
  VPLSCRIIEQVLERRSWQGLDEVVRLLNCSDFAFTDMTKEDKASSESLRLILVVFLGGCTFSEISALRFL
  GREKGYRFIFLTTAVTNSARLMEAMSEVKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289148
• ORF Size: 1770 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289148.1, NP_001276077.1
• RefSeq Size: 2714 bp
• RefSeq ORF: 1773 bp
• Locus ID: 26276
• UniProt ID: Q9H267
• Cytogenetics: 15q26.1
• MW: 68.1 kDa
• Gene Summary: Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and encodes the human ortholog of rat Vps33b which is homologous to the yeast class C Vps33 protein. The mammalian class C vacuolar protein sorting proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Mutations in this gene are associated with arthrogryposis-renal dysfunction-cholestasis syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]