ETFDH (NM_001281738) Human Tagged ORF Clone

CAT#: RC238883

ETFDH (myc-DDK-tagged) - Human electron-transferring-flavoprotein dehydrogenase (ETFDH), transcript variant 3

Product Images

Circular map for RC238883

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ETFDH
• Synonyms: ETFQO; MADD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238883 representing NM_001281738
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAGGTTTGCAGAAGAAGCAGATGTTGTAATAGTTGGTGCAGGCCCTGCAGGGCTCTCTGCAGCTG
  TTCGTCTAAAACAGTTGGCTGTGGCACATGAAAAGGACATCCGTGTGTGTCTAGTGGAGAAAGCTGCCCA
  GATAGGAGCTCATACTCTCTCAGGGGCTTGCCTTGATCCAGGTGCTTTTAAAGAACTCTTCCCAGACTGG
  AAAGAGAAGGGGGCTCCACTTAACACTCCTGTAACAGAAGACAGATTTGGAATTTTAACAGAGAAATACA
  GAATTCCTGTGCCAATTCTTCCAGGGCTTCCAATGAATAATCATGGCAATTACATTGTACGCTTGGGACA
  TTTAGTGAGCTGGATGGGCGAACAAGCAGAAGCCCTTGGTGTTGAAGTATACCCTGGTTATGCAGCTGCT
  GAGGTCCTTTTTCATGATGATGGTAGTGTAAAAGGAATTGCCACTAACGATGTAGGGATACAAAAGGATG
  GTGCACCAAAGGCAACATTTGAGAGAGGACTGGAACTACATGCTAAAGTCACAATTTTTGCAGAAGGTTG
  CCATGGACATCTAGCCAAGCAACTATATAAGAAGTTTGATTTGAGAGCAAATTGTGAACCTCAAACCTAC
  GGGATTGGACTGAAGGAGTTATGGGTTATTGATGAAAAGAACTGGAAACCTGGGAGAGTAGATCACACTG
  TTGGTTGGCCCTTGGACAGACATACCTATGGAGGATCTTTCCTCTATCATTTGAATGAAGGTGAACCCCT
  AGTAGCTCTTGGTCTTGTGGTTGGTCTAGACTATCAGAATCCATACCTGAGTCCATTTAGAGAGTTCCAA
  AGGTGGAAACACCATCCTAGCATTCGGCCAACCTTGGAAGGTGGAAAAAGGATTGCATACGGAGCCAGAG
  CTCTCAATGAAGGTGGCTTTCAGTCTATACCAAAACTCACCTTTCCTGGTGGTTTACTAATTGGTTGTAG
  TCCTGGTTTTATGAATGTTCCCAAGATCAAAGGTACTCACACAGCAATGAAAAGTGGAATTTTAGCAGCA
  GAATCTATTTTTAATCAACTAACTAGTGAAAATCTCCAATCAAAGACAATAGGACTCCATGTAACTGAAT
  ATGAGGACAATTTGAAGAACTCATGGGTATGGAAAGAGCTATATTCTGTTAGAAATATAAGACCGTCCTG
  CCACGGAGTACTGGGTGTATATGGAGGGATGATTTACACTGGAATCTTTTACTGGATATTGAGAGGAATG
  GAGCCGTGGACTCTGAAACATAAAGGTTCTGACTTTGAACGGCTCAAGCCAGCCAAGGATTGCACACCTA
  TTGAGTATCCAAAACCCGATGGACAGATCAGTTTTGACCTCTTGTCATCTGTGGCTCTGAGTGGTACTAA
  TCATGAACATGACCAGCCGGCACACTTAACCTTAAGGGATGACAGTATACCTGTAAATAGAAATCTGTCG
  ATATATGATGGGCCCGAGCAGCGATTCTGTCCTGCAGGAGTTTATGAATTTGTACCTGTGGAACAAGGTG
  ATGGATTTCGGTTACAGATAAATGCTCAGAACTGTGTACATTGTAAAACATGTGATATTAAAGATCCAAG
  TCAGAATATTAACTGGGTGGTACCTGAAGGTGGAGGAGGACCTGCTTACAATGGAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238883 representing NM_001281738
  Red=Cloning site Green=Tags(s)
  
  MERFAEEADVVIVGAGPAGLSAAVRLKQLAVAHEKDIRVCLVEKAAQIGAHTLSGACLDPGAFKELFPDW
  KEKGAPLNTPVTEDRFGILTEKYRIPVPILPGLPMNNHGNYIVRLGHLVSWMGEQAEALGVEVYPGYAAA
  EVLFHDDGSVKGIATNDVGIQKDGAPKATFERGLELHAKVTIFAEGCHGHLAKQLYKKFDLRANCEPQTY
  GIGLKELWVIDEKNWKPGRVDHTVGWPLDRHTYGGSFLYHLNEGEPLVALGLVVGLDYQNPYLSPFREFQ
  RWKHHPSIRPTLEGGKRIAYGARALNEGGFQSIPKLTFPGGLLIGCSPGFMNVPKIKGTHTAMKSGILAA
  ESIFNQLTSENLQSKTIGLHVTEYEDNLKNSWVWKELYSVRNIRPSCHGVLGVYGGMIYTGIFYWILRGM
  EPWTLKHKGSDFERLKPAKDCTPIEYPKPDGQISFDLLSSVALSGTNHEHDQPAHLTLRDDSIPVNRNLS
  IYDGPEQRFCPAGVYEFVPVEQGDGFRLQINAQNCVHCKTCDIKDPSQNINWVVPEGGGGPAYNGM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001281738
• ORF Size: 1668 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001281738.1, NP_001268667.1
• RefSeq Size: 1952 bp
• RefSeq ORF: 1671 bp
• Locus ID: 2110
• Cytogenetics: 4q32.1
• MW: 61.8 kDa
• Gene Summary: This gene encodes a component of the electron-transfer system in mitochondria and is essential for electron transfer from a number of mitochondrial flavin-containing dehydrogenases to the main respiratory chain. Mutations in this gene are associated with glutaric acidemia. Alternatively spliced transcript variants that encode distinct isoforms have been observed. [provided by RefSeq, Aug 2013]