ETFDH (NM_001281738) Human Tagged ORF Clone
CAT#: RC238883
- TrueORF®
ETFDH (myc-DDK-tagged) - Human electron-transferring-flavoprotein dehydrogenase (ETFDH), transcript variant 3
ORF Plasmid: tGFP
"NM_001281738" in other vectors (2)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | ETFDH |
Synonyms | ETFQO; MADD |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC238883 representing NM_001281738
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAGGTTTGCAGAAGAAGCAGATGTTGTAATAGTTGGTGCAGGCCCTGCAGGGCTCTCTGCAGCTG TTCGTCTAAAACAGTTGGCTGTGGCACATGAAAAGGACATCCGTGTGTGTCTAGTGGAGAAAGCTGCCCA GATAGGAGCTCATACTCTCTCAGGGGCTTGCCTTGATCCAGGTGCTTTTAAAGAACTCTTCCCAGACTGG AAAGAGAAGGGGGCTCCACTTAACACTCCTGTAACAGAAGACAGATTTGGAATTTTAACAGAGAAATACA GAATTCCTGTGCCAATTCTTCCAGGGCTTCCAATGAATAATCATGGCAATTACATTGTACGCTTGGGACA TTTAGTGAGCTGGATGGGCGAACAAGCAGAAGCCCTTGGTGTTGAAGTATACCCTGGTTATGCAGCTGCT GAGGTCCTTTTTCATGATGATGGTAGTGTAAAAGGAATTGCCACTAACGATGTAGGGATACAAAAGGATG GTGCACCAAAGGCAACATTTGAGAGAGGACTGGAACTACATGCTAAAGTCACAATTTTTGCAGAAGGTTG CCATGGACATCTAGCCAAGCAACTATATAAGAAGTTTGATTTGAGAGCAAATTGTGAACCTCAAACCTAC GGGATTGGACTGAAGGAGTTATGGGTTATTGATGAAAAGAACTGGAAACCTGGGAGAGTAGATCACACTG TTGGTTGGCCCTTGGACAGACATACCTATGGAGGATCTTTCCTCTATCATTTGAATGAAGGTGAACCCCT AGTAGCTCTTGGTCTTGTGGTTGGTCTAGACTATCAGAATCCATACCTGAGTCCATTTAGAGAGTTCCAA AGGTGGAAACACCATCCTAGCATTCGGCCAACCTTGGAAGGTGGAAAAAGGATTGCATACGGAGCCAGAG CTCTCAATGAAGGTGGCTTTCAGTCTATACCAAAACTCACCTTTCCTGGTGGTTTACTAATTGGTTGTAG TCCTGGTTTTATGAATGTTCCCAAGATCAAAGGTACTCACACAGCAATGAAAAGTGGAATTTTAGCAGCA GAATCTATTTTTAATCAACTAACTAGTGAAAATCTCCAATCAAAGACAATAGGACTCCATGTAACTGAAT ATGAGGACAATTTGAAGAACTCATGGGTATGGAAAGAGCTATATTCTGTTAGAAATATAAGACCGTCCTG CCACGGAGTACTGGGTGTATATGGAGGGATGATTTACACTGGAATCTTTTACTGGATATTGAGAGGAATG GAGCCGTGGACTCTGAAACATAAAGGTTCTGACTTTGAACGGCTCAAGCCAGCCAAGGATTGCACACCTA TTGAGTATCCAAAACCCGATGGACAGATCAGTTTTGACCTCTTGTCATCTGTGGCTCTGAGTGGTACTAA TCATGAACATGACCAGCCGGCACACTTAACCTTAAGGGATGACAGTATACCTGTAAATAGAAATCTGTCG ATATATGATGGGCCCGAGCAGCGATTCTGTCCTGCAGGAGTTTATGAATTTGTACCTGTGGAACAAGGTG ATGGATTTCGGTTACAGATAAATGCTCAGAACTGTGTACATTGTAAAACATGTGATATTAAAGATCCAAG TCAGAATATTAACTGGGTGGTACCTGAAGGTGGAGGAGGACCTGCTTACAATGGAATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC238883 representing NM_001281738
Red=Cloning site Green=Tags(s) MERFAEEADVVIVGAGPAGLSAAVRLKQLAVAHEKDIRVCLVEKAAQIGAHTLSGACLDPGAFKELFPDW KEKGAPLNTPVTEDRFGILTEKYRIPVPILPGLPMNNHGNYIVRLGHLVSWMGEQAEALGVEVYPGYAAA EVLFHDDGSVKGIATNDVGIQKDGAPKATFERGLELHAKVTIFAEGCHGHLAKQLYKKFDLRANCEPQTY GIGLKELWVIDEKNWKPGRVDHTVGWPLDRHTYGGSFLYHLNEGEPLVALGLVVGLDYQNPYLSPFREFQ RWKHHPSIRPTLEGGKRIAYGARALNEGGFQSIPKLTFPGGLLIGCSPGFMNVPKIKGTHTAMKSGILAA ESIFNQLTSENLQSKTIGLHVTEYEDNLKNSWVWKELYSVRNIRPSCHGVLGVYGGMIYTGIFYWILRGM EPWTLKHKGSDFERLKPAKDCTPIEYPKPDGQISFDLLSSVALSGTNHEHDQPAHLTLRDDSIPVNRNLS IYDGPEQRFCPAGVYEFVPVEQGDGFRLQINAQNCVHCKTCDIKDPSQNINWVVPEGGGGPAYNGM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001281738 |
ORF Size | 1668 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001281738.1, NP_001268667.1 |
RefSeq Size | 1952 bp |
RefSeq ORF | 1671 bp |
Locus ID | 2110 |
Cytogenetics | 4q32.1 |
MW | 61.8 kDa |
Gene Summary | This gene encodes a component of the electron-transfer system in mitochondria and is essential for electron transfer from a number of mitochondrial flavin-containing dehydrogenases to the main respiratory chain. Mutations in this gene are associated with glutaric acidemia. Alternatively spliced transcript variants that encode distinct isoforms have been observed. [provided by RefSeq, Aug 2013] |
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