PFTK1 (CDK14) (NM_001287135) Human Tagged ORF Clone

CAT#: RC238411

CDK14 (myc-DDK-tagged) - Human cyclin-dependent kinase 14 (CDK14), transcript variant 1

Product Images

Circular map for RC238411

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PFTK1
• Synonyms: PFTAIRE1; PFTK1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238411 representing NM_001287135
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGACCTCATTGAGCCGCAGCCGGCCGAGAAGATCGGCAAGATGAAGAAGTTGCGGAGAACTTTGT
  CGGAGAGTTTCAGTCGCATTGCTTTGAAGAAAGATGACACCACCTTTGATGAGATATGTGTCACAAAGAT
  GTCTACACGGAACTGCCAGGGAATGGACTCAGTGATCAAACCCCTGGACACAATTCCTGAGGATAAAAAA
  GTCAGAGTTCAGAGGACACAGAGCACTTTTGACCCATTTGAGAAACCAGCTAATCAAGTAAAGAGGGTGC
  ATTCTGAGAACAATGCTTGCATTAACTTTAAGACCTCCTCCACTGGCAAAGAGTCACCTAAAGTTAGGCG
  GCACTCCAGCCCCAGCTCGCCAACAAGTCCCAAATTTGGAAAAGCTGACTCATATGAAAAGCTGGAAAAA
  CTAGGGGAAGGATCTTATGCTACAGTATACAAAGGGAAAAGCAAGGTAAATGGGAAGTTGGTAGCTCTGA
  AGGTGATCAGGCTGCAGGAAGAAGAAGGGACACCTTTCACAGCTATCAGGGAAGCTTCTCTTTTAAAAGG
  ACTAAAACATGCTAACATAGTGCTACTTCATGACATCATCCATACCAAGGAGACGCTGACACTTGTGTTT
  GAATATGTGCACACTGATTTATGTCAGTACATGGACAAGCACCCTGGGGGGCTGCATCCAGATAATGTGA
  AGTTGTTTTTATTTCAGTTGCTGCGAGGTCTGTCTTACATCCACCAGCGTTATATTTTGCACAGAGACCT
  GAAACCACAGAACCTTCTGATCAGTGACACGGGGGAGTTAAAGCTGGCAGATTTCGGTCTTGCAAGAGCA
  AAATCCGTCCCTAGCCACACATACTCCAACGAAGTGGTTACCTTGTGGTACAGACCTCCAGATGTCCTTC
  TAGGCTCAACAGAATATTCCACCTGCCTTGACATGTGGGGAGTAGGTTGCATCTTTGTTGAAATGATCCA
  AGGAGTTGCTGCTTTTCCAGGAATGAAAGACATTCAGGATCAACTTGAACGAATATTTCTGGTTCTTGGA
  ACACCAAATGAGGACACATGGCCTGGAGTTCATTCTTTACCACATTTTAAGCCAGAACGCTTTACCCTGT
  ACAGCTCTAAAAACCTTAGACAAGCATGGAATAAGCTCAGCTATGTGAACCATGCAGAGGACCTGGCCTC
  CAAGCTCCTACAATGTTCCCCAAAGAACAGACTGTCGGCACAGGCTGCCTTGAGCCACGAGTATTTTAGT
  GACCTGCCGCCACGGCTATGGGAACTCACCGACATGTCTTCTATTTTTACTGTCCCAAATGTGAGATTGC
  AACCAGAAGCTGGAGAAAGCATGCGGGCCTTTGGGAAAAACAATAGTTATGGCAAAAGTCTATCAAACAG
  CAAGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238411 representing NM_001287135
  Red=Cloning site Green=Tags(s)
  
  MCDLIEPQPAEKIGKMKKLRRTLSESFSRIALKKDDTTFDEICVTKMSTRNCQGMDSVIKPLDTIPEDKK
  VRVQRTQSTFDPFEKPANQVKRVHSENNACINFKTSSTGKESPKVRRHSSPSSPTSPKFGKADSYEKLEK
  LGEGSYATVYKGKSKVNGKLVALKVIRLQEEEGTPFTAIREASLLKGLKHANIVLLHDIIHTKETLTLVF
  EYVHTDLCQYMDKHPGGLHPDNVKLFLFQLLRGLSYIHQRYILHRDLKPQNLLISDTGELKLADFGLARA
  KSVPSHTYSNEVVTLWYRPPDVLLGSTEYSTCLDMWGVGCIFVEMIQGVAAFPGMKDIQDQLERIFLVLG
  TPNEDTWPGVHSLPHFKPERFTLYSSKNLRQAWNKLSYVNHAEDLASKLLQCSPKNRLSAQAALSHEYFS
  DLPPRLWELTDMSSIFTVPNVRLQPEAGESMRAFGKNNSYGKSLSNSKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001287135
• ORF Size: 1407 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001287135.2
• RefSeq Size: 5146 bp
• RefSeq ORF: 1410 bp
• Locus ID: 5218
• UniProt ID: O94921
• Cytogenetics: 7q21.13
• Protein Families: Druggable Genome, Protein Kinase
• MW: 53.5 kDa
• Gene Summary: PFTK1 is a member of the CDC2 (MIM 116940)-related protein kinase family (Yang and Chen, 2001 [PubMed 11313143]).[supplied by OMIM, Mar 2008]