LIPI (NM_001302999) Human Tagged ORF Clone

CAT#: RC238186

LIPI (myc-DDK-tagged) - Human lipase, member I (LIPI), transcript variant deltaE5

Product Images

Circular map for RC238186

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LIPI
• Synonyms: CT17; LPDL; mPA-PLA1 beta; PLA1C; PRED5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238186 representing NM_001302999
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGTATACATTTTTCTTTGTTTGATGTGCTGGGTGAGATCTGATAATAAAAGACCATGCCTTGAAT
  TCTCTCAGCTAAGTGTAAAGGATTCCTTCAGAGATTTATTTATTCCGAGAATAGAGACCATTCTGATGAT
  GTATACAAGGAACAACCTAAACTGTGCTGAGCCACTGTTTGAACAAAATAACTCACTTAATGTTAATTTC
  AACACACAAAAGAAAACAGTCTGGCTTATTCACGGATACAGACCAGTAGGCTCCATCCCATTATGGCTTC
  AGAACTTCGTAAGGATTTTGCTGAATGAAGAAGATATGAATGTAATTGTAGTAGACTGGAGCCGGGGTGC
  TACAACTTTTATTTATAATAGAGCAGTTAAAAACACCAGAAAAGTTGCTGTGAGTTTGAGTGTGCACATT
  AAAAATCTTTTGAAGCATGGTGCATCTCTTGACAATTTTCATTTCATAGGTGTGAGCTTAGGGGCTCATA
  TCAGTGGATTTGTTGGAAAGATATTTCATGGTCAACTTGGAAGAATAACAGGTCTTGACCCTGCTGGGCC
  AAGGTTCTCCAGAAAACCACCATATAGCAGATTAGATTACACGGATGCAAAGTTTGTGGATGTCATCCAT
  TCTGACTCCAATGGAATTCAATTCATTAAATGCAACCACCAGAGAGCAGTTCACTTGTTCATGGCATCTT
  TAGAAACAAACTGCAATTTTATTTCATTTCCTTGTCGTTCATACAAAGATTACAAGACTAGCTTATGTGT
  GGACTGTGACTGTTTTAAGGAAAAATCATGTCCTCGGCTGGGTTATCAAGCCAAGCTATTTAAAGGTGTT
  TTAAAAGAAAGGATGGAAGGAAGACCTCTTAGGACCACTGTGTTTTTGGATACAAGTGGTACATATCCAT
  TCTGTACCTATTATTTTGTTCTCAGTATAATTGTTCCAGATAAAACTATGATGGATGGCTCGTTTTCATT
  TAAATTATTAAATCAGCTTGGAATGATTGAAGAGCCAAGGCTTTATGAAAAGAACAAACCATTTTATAAA
  CTTCAAGAAGTCAAGATTCTTGCTCAATTTTATAATGACTTTGTAAATATTTCAAGCATTGGTTTGACAT
  ATTTCCAGAGCTCAAATCTGCAGTGTTCCACATGCACATACAAGATCCAGAGTCTCATGTTAAAATCACT
  TACATACCCAGAAAGACCACCACTTTGCAGGTATAATATTGTACTTAAAGACAGAGAGGAAGTGTTTCTT
  AATCCAAACACATGTACACCAAAGAACACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238186 representing NM_001302999
  Red=Cloning site Green=Tags(s)
  
  MRVYIFLCLMCWVRSDNKRPCLEFSQLSVKDSFRDLFIPRIETILMMYTRNNLNCAEPLFEQNNSLNVNF
  NTQKKTVWLIHGYRPVGSIPLWLQNFVRILLNEEDMNVIVVDWSRGATTFIYNRAVKNTRKVAVSLSVHI
  KNLLKHGASLDNFHFIGVSLGAHISGFVGKIFHGQLGRITGLDPAGPRFSRKPPYSRLDYTDAKFVDVIH
  SDSNGIQFIKCNHQRAVHLFMASLETNCNFISFPCRSYKDYKTSLCVDCDCFKEKSCPRLGYQAKLFKGV
  LKERMEGRPLRTTVFLDTSGTYPFCTYYFVLSIIVPDKTMMDGSFSFKLLNQLGMIEEPRLYEKNKPFYK
  LQEVKILAQFYNDFVNISSIGLTYFQSSNLQCSTCTYKIQSLMLKSLTYPERPPLCRYNIVLKDREEVFL
  NPNTCTPKNT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001302999
• ORF Size: 1290 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302999.2
• RefSeq Size: 1519 bp
• RefSeq ORF: 1293 bp
• Locus ID: 149998
• UniProt ID: Q6XZB0
• Cytogenetics: 21q11.2
• Protein Families: Secreted Protein
• MW: 50.2 kDa
• Gene Summary: The protein encoded by this gene is a phospholipase that hydrolyzes phosphatidic acid to produce lysophosphatidic acid. Defects in this gene are a cause of susceptibility to familial hypertrigliceridemia. This gene is also expressed at high levels in Ewing family tumor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]