ANKRD26 (NM_001256053) Human Tagged ORF Clone

CAT#: RC235330

ANKRD26 (Myc-DDK tagged) - Homo sapiens ankyrin repeat domain 26 (ANKRD26), transcript variant 2

Product Images

Circular map for RC235330

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANKRD26
• Synonyms: bA145E8.1; THC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235330 representing NM_001256053
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAGATTTTTAGTAAGAAGGGCGAGTCGCCCTTGGGCTCCTTCGCGCGGCGGCAGAGGAGCAGCG
  CGGGAGGCGGGGGCGAGCCGGGGGAGGGCGCCTACTCGCAGCCCGGCTACCACGTCCGAGACCGAGATCT
  CGGCAAGATCCACAAAGCTGCCAGCGCGGGTAATGTGGCGAAAGTGCAGCAGATCCTTTTGCTCAGGAAG
  AATGGCTTGAACGATAGAGACAAGATGAACAGGACGGCTCTACATTTGGCCTGTGCCAATGGTCATCCAG
  AAGTAGTAACTCTCCTGGTGGACAGAAAATGCCAGCTCAATGTCTGTGACAACGAAAACAGGACAGCTCT
  GATGAAGGCTGTACAATGCCAGGAAGAGAAATGTGCAACTATTCTGCTAGAACATGGTGCTGATCCAAAT
  CTTGCGGATGTCCATGGCAACACTGCTCTTCACTATGCTGTCTATAATGAGGACATATCAGTAGCAACAA
  AGCTGCTTTTGTATGATGCAAATATTGAAGCAAAAAACAAGGATGACCTCACACCACTTTTACTTGCAGT
  AAGTGGAAAAAAGCAGCAAATGGTGGAATTTTTAATAAAGAAAAAAGCAAATGTAAATGCAGTAGATAAG
  TTGGAAAGCAGTCACCAACTAATTTCAGAATATAAAGAAGAAAGGATACCTAAACATTCTTCTCAAAATA
  GTAATTCAGTGGATGAAAGCTCTGAAGACTCCTTAAGCAGGCTTTCTGGCAAACCGGGTGTTGATGATTC
  ATGGCCTACCTCAGATGACGAAGACCTCAATTTTGATACTAAGAATGTCCCAAAACCAAGCTTAGCAAAG
  CTAATGACTGCTTCTCAGCAATCCAGGAAAAATTTAGAAGCAACATATGGCACTGTGAGAACAGGAAATA
  GAACTTTGTTTGAGGATAGAGATTCCGATAGTCAAGATGAAGTTGTGGTTGAAAGCCTTCCTACAACATC
  AATCAAAGTCCAGTGCTTTTCTCATCCTACCTATCAATCACCTGACCTTCTTCCAAAACCTTCCCACAAG
  TCGTTAGCAAACCCTGGTCTTATGAAGGAAGAACCAACAAAGCCAGGCATTGCAAAAAAAGAAAATGGTA
  TTGATATTATTGAAAGTGCTCCACTAGAGCAAACAAATAATGACAATTTGACTTATGTTGATGAAGTGCA
  CAAAAATAATAGAAGTGATATGATGTCCGCATTAGGATTAGGACAAGAGGAAGATATAGAATCACCTTGG
  GATTCTGAGAGTATCTCTGAGAATTTTCCACAGAAGTATGTTGATCCTTTAGCTGGGGCTGCAGACGGAA
  AAGAAAAAAATATAGGAAATGAACAAGCAGAAGATGTGTTTTATATACCTTCTTGCATGAGTGGATCAAG
  AAACTTTAAGATGGCTAAACTAGAGGATACAAGAAATGTAGGCATGCCAGTAGCCCACATGGAGTCTCCT
  GAGAGATATCTTCACTTGAAGCCTACCATTGAAATGAAAGATTCTGTTCCAAATAAAGCAGGAGGAATGA
  AGGATGTACAAACATCCAAAGCAGCTGAACATGACTTAGAAGTAGCATCAGAAGAAGAGCAAGAAAGGGA
  AGGGAGTGAAAATAACCAGCCACAGGTTGAAGAAGAAAGGAAAAAACACAGAAATAATGAAATGGAAGTA
  TCAGCAAACATACATGATGGTGCTACTGATGATGCTGAAGATGATGATGATGATGATGGATTAATTCAAA
  AAAGAAAGAGTGGAGAAACTGATCATCAGCAATTTCCCAGGAAGGAAAATAAAGAGTATGCTAGTGGTCC
  TGCCTTGCAAATGAAGGAAGTAAAGAGCACTGAAAAAGAAAAACGGACCTCGAAAGAATCTGTGAATTCA
  CCAGTGTTTGGGAAGGCCAGTTTACTAACTGGTGGCCTGCTACAAGTGGATGATGACAGCAGTTTAAGTG
  AAATAGATGAGGATGAAGGAAGGCCTACTAAGAAAACATCTAATGAAAAGAACAAGGTCAAAAACCAAAT
  ACAGTCTATGGATGATGTTGATGACTTAACTCAGTCATCTGAAACAGCCTCAGAGGATTGTGAGCTACCC
  CACTCTAGTTACAAGAATTTTATGTTGCTCATTGAACAACTTGGAATGGAGTGTAAAGATTCTGTTAGCC
  TATTGAAAATCCAGGATGCAGCTCTTTCATGTGAAAGATTATTAGAACTTAAAAAAAATCACTGTGAACT
  ACTTACAGTAAAAATTAAAAAAATGGAAGACAAGGTTAATGTACTACAAAGGGAGCTATCTGAAACAAAA
  GAAATAAAATCACAGTTAGAGCATCAAAAAGTTGAATGGGAACGAGAACTGTGCTCTTTGAGATTTAGCT
  TAAACCAAGAAGAAGAGAAGAGAAGAAATGCTGATACGTTGTATGAAAAAATTAGGGAACAGTTAAGAAG
  AAAAGAAGAGCAATATAGGAAAGAAGTTGAAGTGAAACAACAGCTTGAACTGAGTCTCCAAACACTGGAG
  ATGGAATTGAGGACTGTAAAAAGTAATTTGAATCAGGTCGTTCAAGAGCGAAATGACGCTCAGAGGCAAC
  TTTCTCGAGAACAGAATGCCAGAATGTTACAAGATGGAATTCTGACCAATCACCTTTCCAAACAAAAGGA
  GATTGAAATGGCTCAAAAGAAAATGAATTCTGAGAATTCTCATAGTCATGAAGAAGAAAAAGACCTATCG
  CATAAAAATAGCATGTTGCAGGAAGAAATTGCTATGCTAAGACTAGAAATAGACACAATAAAAAATCAAA
  ACCAGGAAAAAGAAAAGAAATGTTTTGAGGACCTTAAAATTGTAAAAGAAAAGAATGAAGACCTTCAGAA
  GACTATAAAACAGAATGAGGAAACATTAACACAAACAATATCCCAGTATAATGGACGGCTTAGTGTTCTG
  ACAGCTGAGAATGCAATGCTAAATTCTAAACTGGAGAATGAAAAGCAAAGCAAGGAAAGACTGGAAGCAG
  AAGTTGAATCATACCATTCTAGATTGGCTGCTGCTATACATGATCGTGATCAAAGTGAGACATCAAAAAG
  AGAACTAGAACTTGCTTTCCAGAGAGCAAGAGATGAATGTTCTCGTTTACAGGACAAAATGAATTTTGAT
  GTGTCTAACCTAAAAGATAACAATGAGATTCTTTCTCAACAACTATTTAAAACTGAAAGTAAACTCAATA
  GCCTAGAAATTGAGTTCCATCACACGAGAGATGCCCTCAGAGAAAAGACTTTGGGTTTAGAACGGGTACA
  AAAGGACCTAAGCCAAACACAGTGTCAAATGAAGGAAATGGAACAAAAGTATCAAAATGAACAAGTTAAA
  GTGAATAAATACATTGGAAAGCAGGAGTCTGTAGAGGAGAGATTGTCTCAACTACAAAGTGAGAATATGT
  TGCTTCGACAACAACTGGATGATGCCCACAACAAGGCTGACAATAAAGAGAAGACAGTGATTAATATCCA
  AGACCAGTTTCATGCTATTGTGCAAAAACTTCAAGCTGAGAGTGAAAAGCAAAGTCTTCTGCTAGAAGAA
  AGAAATAAGGAGTTAATCAGTGAATGTAATCACTTAAAAGAAAGACAGTATCAATATGAAAATGAAAAGG
  CAGAAAGAGAAGTTGTTGTGAGACAACTTCAACAAGAACTAGCTGATACCCTAAAAAAACAATCTATGTC
  AGAGGCTTCACTGGAGGTTACGTCACGTTATCGTATTAATTTAGAAGATGAGACACAGGATTTAAAGAAG
  AAATTAGGTCAAATCAGAAATCAATTGCAAGAAGCACAGGATCGACATACAGAAGCTGTCAGATGTGCTG
  AGAAGATGCAAGATCACAAGCAAAAGCTTGAAAAAGATAATGCCAAGTTAAAAGTTACAGTCAAAAAGCA
  AATGGACAAAATTGAGGAGCTTCAGAAAAACCTGTTAAATGCAAATTTGTCTGAAGATGAAAAGGAACAA
  TTAAAGAAACTTATGGAATTAAAACAGTCACTGGAATGTAATTTGGATCAAGAAATGAAGAAAAATGTTG
  AATTAGAAAGAGAGATAACTGGATTTAAGAACCTCTTAAAAATGACAAGAAAGAAGTTAAATGAATATGA
  AAATGGAGAATTTAGTTTCCATGGAGATTTAAAAACTAGTCAATTTGAAATGGATATTCAGATTAATAAG
  CTAAAACATAAGATTGATGATCTTACAGCAGAACTGGAGACTGCAGGTTCAAAATGTCTACATCTGGATA
  CAAAGAACCAAATTCTTCAAGAGGAGTTGTTATCTATGAAAACAGTACAAAAGAAATGTGAAAAACTACA
  GAAGAATAAAAAGAAGTTGGAACAAGAAGTGATCAACCTGAGAAGTCATATAGAAAGGAATATGGTAGAA
  CTTGGTCAAGTCAAACAGTATAAACAGGAGATTGAAGAAAGAGCAAGACAGGAAATAGCAGAAAAATTAA
  AAGAAGTCAATCTATTTTTACAGGCACAAGCAGCATCTCAAGAAAACTTAGAGCAGTTTAGAGAGAATAA
  TTTTGCTTCAATGAAAAGTCAGATGGAACTCAGAATTAAAGATCTGGAATCTGAACTCTCCAAAATAAAA
  ACTTCTCAAGAAGACTTTAATAAAACCGAACTGGAAAAATATAAGCAACTCTATCTAGAAGAATTAAAAG
  TTAGAAAATCTTTGTCAAGTAAACTAACCAAAACTAATGAGAGGCTAGCAGAGGTCAACACCAAACTTCT
  TGTGGAAAAACAGCAGAGCAGATCTTTGTTCACCACTCTCACTACCAGGCCAGTCATGGAGCCACCTTGT
  GTGGGAAATCTTAATAATAGTTTAGATCTCAACAGAAAACTTATTCCAAGAGAAAACTTAGTGATCTCTA
  CCTCAAATCCACGGGCTTCAAATAATAGCATGGAGAACTACTTGAGCAAGATGCAGCAGGAGTTGGAAAA
  AAATATAACTAGAGAACTCAAAGAAGCTGCTGCTGAATTGGAATCTGGATCAATAGCTTCCCCTCTAGGG
  TCTACTGATGAGTCAAATCTAAATCAAGATCTAGTTTGGAAAGCATCAAGAGAATATGTACAGGTTTTAA
  AGAAAAATTATATGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235330 representing NM_001256053
  Red=Cloning site Green=Tags(s)
  
  MKKIFSKKGESPLGSFARRQRSSAGGGGEPGEGAYSQPGYHVRDRDLGKIHKAASAGNVAKVQQILLLRK
  NGLNDRDKMNRTALHLACANGHPEVVTLLVDRKCQLNVCDNENRTALMKAVQCQEEKCATILLEHGADPN
  LADVHGNTALHYAVYNEDISVATKLLLYDANIEAKNKDDLTPLLLAVSGKKQQMVEFLIKKKANVNAVDK
  LESSHQLISEYKEERIPKHSSQNSNSVDESSEDSLSRLSGKPGVDDSWPTSDDEDLNFDTKNVPKPSLAK
  LMTASQQSRKNLEATYGTVRTGNRTLFEDRDSDSQDEVVVESLPTTSIKVQCFSHPTYQSPDLLPKPSHK
  SLANPGLMKEEPTKPGIAKKENGIDIIESAPLEQTNNDNLTYVDEVHKNNRSDMMSALGLGQEEDIESPW
  DSESISENFPQKYVDPLAGAADGKEKNIGNEQAEDVFYIPSCMSGSRNFKMAKLEDTRNVGMPVAHMESP
  ERYLHLKPTIEMKDSVPNKAGGMKDVQTSKAAEHDLEVASEEEQEREGSENNQPQVEEERKKHRNNEMEV
  SANIHDGATDDAEDDDDDDGLIQKRKSGETDHQQFPRKENKEYASGPALQMKEVKSTEKEKRTSKESVNS
  PVFGKASLLTGGLLQVDDDSSLSEIDEDEGRPTKKTSNEKNKVKNQIQSMDDVDDLTQSSETASEDCELP
  HSSYKNFMLLIEQLGMECKDSVSLLKIQDAALSCERLLELKKNHCELLTVKIKKMEDKVNVLQRELSETK
  EIKSQLEHQKVEWERELCSLRFSLNQEEEKRRNADTLYEKIREQLRRKEEQYRKEVEVKQQLELSLQTLE
  MELRTVKSNLNQVVQERNDAQRQLSREQNARMLQDGILTNHLSKQKEIEMAQKKMNSENSHSHEEEKDLS
  HKNSMLQEEIAMLRLEIDTIKNQNQEKEKKCFEDLKIVKEKNEDLQKTIKQNEETLTQTISQYNGRLSVL
  TAENAMLNSKLENEKQSKERLEAEVESYHSRLAAAIHDRDQSETSKRELELAFQRARDECSRLQDKMNFD
  VSNLKDNNEILSQQLFKTESKLNSLEIEFHHTRDALREKTLGLERVQKDLSQTQCQMKEMEQKYQNEQVK
  VNKYIGKQESVEERLSQLQSENMLLRQQLDDAHNKADNKEKTVINIQDQFHAIVQKLQAESEKQSLLLEE
  RNKELISECNHLKERQYQYENEKAEREVVVRQLQQELADTLKKQSMSEASLEVTSRYRINLEDETQDLKK
  KLGQIRNQLQEAQDRHTEAVRCAEKMQDHKQKLEKDNAKLKVTVKKQMDKIEELQKNLLNANLSEDEKEQ
  LKKLMELKQSLECNLDQEMKKNVELEREITGFKNLLKMTRKKLNEYENGEFSFHGDLKTSQFEMDIQINK
  LKHKIDDLTAELETAGSKCLHLDTKNQILQEELLSMKTVQKKCEKLQKNKKKLEQEVINLRSHIERNMVE
  LGQVKQYKQEIEERARQEIAEKLKEVNLFLQAQAASQENLEQFRENNFASMKSQMELRIKDLESELSKIK
  TSQEDFNKTELEKYKQLYLEELKVRKSLSSKLTKTNERLAEVNTKLLVEKQQSRSLFTTLTTRPVMEPPC
  VGNLNNSLDLNRKLIPRENLVISTSNPRASNNSMENYLSKMQQELEKNITRELKEAAAELESGSIASPLG
  STDESNLNQDLVWKASREYVQVLKKNYMI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256053
• ORF Size: 5127 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256053.2
• RefSeq Size: 6776 bp
• RefSeq ORF: 5130 bp
• Locus ID: 22852
• UniProt ID: Q9UPS8
• Cytogenetics: 10p12.1
• MW: 196.8 kDa
• Gene Summary: This gene encodes a protein containing N-terminal ankyrin repeats which function in protein-protein interactions. Mutations in this gene are associated with autosomal dominant thrombocytopenia-2. Pseudogenes of this gene are found on chromosome 7, 10, 13 and 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]