ARID5B (NM_001244638) Human Tagged ORF Clone
CAT#: RC235069
- TrueORF®
ARID5B (Myc-DDK tagged) - Homo sapiens AT rich interactive domain 5B (MRF1-like) (ARID5B), transcript variant 2
ORF Plasmid: tGFP
"NM_001244638" in other vectors (2)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | ARID5B |
Synonyms | DESRT; MRF-2; MRF2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC235069 representing NM_001244638
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCAAATCTTAAAGGCAGACCACGCAAAAAGAAACCATGCCCACAAAGAAGAGATTCATTCAGTG GTGTTAAGGATTCCAACAACAATTCCGATGGCAAAGCCGTTGCCAAGGTGAAATGTGAGGCCAGGTCAGC CTTGACCAAGCCGAAGAATAACCATAACTGTAAAAAAGTCTCAAATGAAGAAAAACCAAAGGTTGCCATT GGTGAAGAGTGCAGGGCAGATGAACAAGCCTTCTTGGTGGCACTTTATAAATACATGAAAGAAAGGAAAA CGCCGATAGAACGAATACCCTATTTAGGTTTTAAACAGATTAACCTTTGGACTATGTTTCAAGCTGCTCA AAAACTGGGAGGATATGAAACAATAACAGCCCGCCGTCAGTGGAAACATATTTATGATGAATTAGGCGGT AATCCTGGGAGCACCAGCGCTGCCACTTGTACCCGCAGACATTATGAAAGATTAATCCTACCATATGAAA GATTTATTAAAGGAGAAGAAGATAAGCCCCTGCCTCCAATCAAACCTCGGAAACAGGAGAACAGTTCACA GGAAAATGAGAACAAAACAAAAGTATCTGGAACCAAACGCATCAAACATGAAATACCTAAAAGCAAGAAA GAAAAAGAAAATGCCCCAAAGCCCCAGGATGCAGCAGAGGTTTCATCAGAGCAAGAAAAAGAACAAGAGA CTTTAATAAGCCAGAAAAGCATCCCTGAGCCTCTCCCAGCAGCAGACATGAAGAAAAAAATAGAAGGGTA TCAGGAATTTTCAGCGAAGCCCCTGGCATCCAGAGTAGACCCAGAGAAGGACAACGAAACAGACCAAGGT TCCAACAGTGAGAAGGTGGCAGAGGAGGCGGGAGAGAAGGGGCCCACACCTCCACTCCCAAGTGCTCCTC TGGCCCCAGAAAAAGATTCAGCCTTGGTCCCTGGGGCCAGCAAACAGCCACTCACCTCTCCTAGTGCCCT GGTGGACTCAAAACAAGAATCCAAACTGTGCTGTTTTACAGAGAGCCCTGAAAGTGAACCCCAAGAAGCA TCCTTCCCCAGCTTCCCCACCACACAGCCACCGCTGGCAAACCAGAATGAGACGGAGGATGACAAACTGC CCGCCATGGCAGATTACATTGCCAACTGCACCGTGAAGGTGGACCAGCTGGGCAGTGACGACATCCACAA TGCGCTCAAGCAGACCCCAAAGGTCCTTGTGGTCCAGTCGTTTGACATGTTCAAAGACAAAGACCTGACT GGGCCCATGAACGAGAACCATGGACTTAATTACACGCCCCTGCTCTACTCTAGGGGCAACCCAGGCATCA TGTCCCCACTGGCCAAGAAAAAGCTTTTGTCCCAAGTGAGTGGGGCCAGCCTCTCCAGCAGCTACCCTTA TGGCTCCCCACCCCCTTTGATCAGCAAAAAGAAACTGATTGCTAGGGATGACTTGTGTTCCAGTTTGTCC CAGACCCACCATGGCCAAAGCACTGACCATATGGCGGTCAGCCGGCCATCAGTGATTCAGCACGTCCAGA GTTTCAGAAGCAAGCCCTCGGAAGAGAGAAAGACCATCAATGACATCTTTAAGCATGAGAAACTGAGTCG ATCAGATCCCCACCGCTGCAGCTTCTCCAAGCATCACCTTAACCCCCTTGCTGACTCCTACGTCCTGAAG CAAGAAATTCAGGAGGGCAAGGATAAACTCTTAGAGAAAAGGGCCCTCCCCCATTCCCACATGCCTAGCT TCCTGGCTGACTTCTACTCGTCCCCTCATCTCCATAGCCTCTACAGACACACCGAGCACCATCTTCATAA TGAACAGACATCCAAATACCCTTCCAGGGACATGTACAGGGAATCGGAAAACAGTTCTTTTCCTTCCCAC AGACACCAAGAAAAGCTCCATGTAAATTATCTCACGTCCCTGCACCTGCAAGACAAAAAGTCGGCGGCAG CAGAAGCCCCTACGGATGATCAGCCTACAGATCTGAGCCTTCCCAAGAACCCGCACAAACCTACCGGCAA GGTCCTGGGCCTGGCTCATTCCACCACAGGGCCCCAGGAGAGCAAAGGCATCTCCCAGTTCCAGGTCTTA GGCAGCCAGAGTCGAGACTGTCACCCCAAAGCCTGTCGGGTATCACCCATGACCATGTCAGGCCCTAAAA AATACCCTGAATCGCTTTCAAGATCAGGAAAACCTCACCATGTGAGACTGGAGAATTTCAGGAAGATGGA AGGCATGGTCCACCCAATCCTGCACCGGAAAATGAGCCCGCAGAACATTGGGGCGGCGCGGCCGATCAAG CGCAGCCTGGAGGATTTGGACCTTGTGATTGCAGGGAAAAAGGCCCGGGCAGTGTCTCCCTTAGACCCAT CCAAGGAGGTCTCTGGGAAGGAGAAGGCCTCTGAGCAGGAGAGTGAAGGCAGCAAAGCAGCGCACGGTGG GCATTCCGGGGGCGGATCAGAAGGCCACAAGCTTCCCCTCTCCTCCCCTATCTTCCCAGGTCTGTATTCC GGGAGCCTGTGTAACTCGGGCCTCAACTCCAGGCTCCCGGCTGGGTATTCTCATTCTCTGCAGTACTTGA AAAACCAGACTGTGCTTTCTCCACTCATGCAGCCCCTGGCTTTCCACTCGCTTGTGATGCAAAGAGGAAT TTTTACATCACCGACAAATTCTCAGCAGCTGTACAGACACTTGGCTGCGGCTACACCTGTAGGAAGTTCA TATGGGGACCTTTTGCATAACAGCATTTACCCTTTAGCTGCTATAAATCCTCAAGCTGCCTTTCCATCTT CCCAGCTGTCATCCGTGCACCCCAGTACAAAACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC235069 representing NM_001244638
Red=Cloning site Green=Tags(s) MAPNLKGRPRKKKPCPQRRDSFSGVKDSNNNSDGKAVAKVKCEARSALTKPKNNHNCKKVSNEEKPKVAI GEECRADEQAFLVALYKYMKERKTPIERIPYLGFKQINLWTMFQAAQKLGGYETITARRQWKHIYDELGG NPGSTSAATCTRRHYERLILPYERFIKGEEDKPLPPIKPRKQENSSQENENKTKVSGTKRIKHEIPKSKK EKENAPKPQDAAEVSSEQEKEQETLISQKSIPEPLPAADMKKKIEGYQEFSAKPLASRVDPEKDNETDQG SNSEKVAEEAGEKGPTPPLPSAPLAPEKDSALVPGASKQPLTSPSALVDSKQESKLCCFTESPESEPQEA SFPSFPTTQPPLANQNETEDDKLPAMADYIANCTVKVDQLGSDDIHNALKQTPKVLVVQSFDMFKDKDLT GPMNENHGLNYTPLLYSRGNPGIMSPLAKKKLLSQVSGASLSSSYPYGSPPPLISKKKLIARDDLCSSLS QTHHGQSTDHMAVSRPSVIQHVQSFRSKPSEERKTINDIFKHEKLSRSDPHRCSFSKHHLNPLADSYVLK QEIQEGKDKLLEKRALPHSHMPSFLADFYSSPHLHSLYRHTEHHLHNEQTSKYPSRDMYRESENSSFPSH RHQEKLHVNYLTSLHLQDKKSAAAEAPTDDQPTDLSLPKNPHKPTGKVLGLAHSTTGPQESKGISQFQVL GSQSRDCHPKACRVSPMTMSGPKKYPESLSRSGKPHHVRLENFRKMEGMVHPILHRKMSPQNIGAARPIK RSLEDLDLVIAGKKARAVSPLDPSKEVSGKEKASEQESEGSKAAHGGHSGGGSEGHKLPLSSPIFPGLYS GSLCNSGLNSRLPAGYSHSLQYLKNQTVLSPLMQPLAFHSLVMQRGIFTSPTNSQQLYRHLAAATPVGSS YGDLLHNSIYPLAAINPQAAFPSSQLSSVHPSTKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001244638 |
ORF Size | 2835 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001244638.2 |
RefSeq Size | 7032 bp |
RefSeq ORF | 2838 bp |
Locus ID | 84159 |
UniProt ID | Q14865 |
Cytogenetics | 10q21.2 |
MW | 105.1 kDa |
Gene Summary | This gene encodes a member of the AT-rich interaction domain (ARID) family of DNA binding proteins. The encoded protein forms a histone H3K9Me2 demethylase complex with PHD finger protein 2 and regulates the transcription of target genes involved in adipogenesis and liver development. This gene also plays a role in cell growth and differentiation of B-lymphocyte progenitors, and single nucleotide polymorphisms in this gene are associated with acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
{0} Product Review(s)
Be the first one to submit a review