DNAJC6 (NM_001256865) Human Tagged ORF Clone
CAT#: RC235005
- TrueORF®
DNAJC6 (Myc-DDK tagged) - Homo sapiens DnaJ (Hsp40) homolog, subfamily C, member 6 (DNAJC6), transcript variant 3
ORF Plasmid: tGFP
"NM_001256865" in other vectors (2)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | DNAJC6 |
Synonyms | DJC6; PARK19 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC235005 representing NM_001256865
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCAGCTATGGGGGAGGTCTCTTTGACATGGTAAAAGGAGGTGCAGGGAGGCTCTTTAGTAACC TAAAGGACAACTTGAAAGACACCCTCAAAGACACATCTTCTAGAGTGATACAATCTGTGACCAGCTACAC AAAGGGAGATTTAGACTTCACTTATGTTACCTCCAGAATTATTGTGATGTCCTTTCCTCTGGACAATGTT GACATAGGATTCAGGAATCAGGTTGATGACATTCGAAGCTTTTTGGATTCCAGACATCTTGACCACTACA CAGTATACAATCTGTCACCTAAGTCTTATCGAACTGCCAAGTTTCACAGCCGGGTCTCAGAATGCAGTTG GCCCATTAGGCAGGCTCCCAGTCTGCACAACCTTTTTGCTGTGTGTCGGAATATGTATAACTGGCTACTG CAGAATCCCAAAAATGTCTGTGTTGTCCACTGCTTGGATGGACGGGCGGCATCATCAATTCTGGTTGGTG CTATGTTCATTTTCTGTAATCTCTACTCTACTCCTGGCCCAGCCATTCGATTGCTATATGCAAAGCGACC AGGAATTGGACTTTCACCATCCCATAGGAGATACCTGGGCTATATGTGTGACCTACTGGCAGACAAGCCC TACCGCCCTCACTTCAAGCCTCTCACAATTAAGTCGATCACTGTCAGTCCAATACCCTTTTTCAACAAAC AGAGGAATGGATGTCGCCCTTACTGTGATGTACTCATTGGAGAAACCAAAATATATTCGACTTGCACAGA TTTTGAACGAATGAAAGAATATCGTGTCCAAGATGGAAAAATCTTCATTCCCTTGAACATCACTGTGCAA GGAGACGTGGTTGTTTCCATGTATCACTTGAGGTCAACCATTGGGAGCCGGCTACAGGCTAAGGTGACCA ACACACAGATATTCCAGCTTCAGTTTCACACTGGATTCATACCACTGGACACAACAGTTTTAAAGTTCAC CAAGCCTGAGTTAGATGCATGTGATGTACCAGAAAAATATCCTCAGCTATTTCAGGTGACACTGGATGTA GAACTACAGCCCCATGACAAAGTAATAGACTTAACTCCACCATGGGAACATTACTGCACAAAAGATGTCA ATCCCAGCATCCTCTTCTCTTCTCACCAGGAACATCAAGATACGCTGGCCTTAGGAGGACAGGCTCCAAT AGATATCCCTCCAGACAACCCCAGGCATTACGGACAAAGTGGTTTCTTTGCCTCTCTCTGTTGGCAAGAT CAGAAATCGGAGAAGTCATTCTGTGAGGAGGACCACGCTGCCCTAGTGAATCAGGAAAGTGAGCAATCAG ATGATGAACTTCTGACACTTTCCAGTCCGCATGGCAATGCCAATGGTGACAAGCCTCATGGAGTCAAGAA GCCCAGCAAAAAGCAGCAGGAGCCAGCAGCCCCTCCACCCCCTGAGGATGTGGACCTTTTGGGCCTGGAA GGGTCTGCAATGAGTAACAGCTTCTCTCCGCCAGCGGCTCCTCCCACCAATTCTGAACTACTGAGTGACC TGTTTGGGGGTGGAGGTGCAGCTGGTCCCACCCAGGCTGGACAGTCAGGAGTGGAAGATGTGTTTCATCC TAGTGGACCTGCGTCTACCCAGTCAACACCACGCCGCTCTGCCACCTCCACCTCTGCGTCTCCAACCCTA AGAGTGGGAGAAGGTGCCACCTTTGACCCATTTGGAGCACCTTCTAAACCATCAGGTCAGGATTTGCTGG GTTCTTTTCTGAACACATCCAGTGCTTCCAGTGACCCCTTTCTCCAGCCAACAAGAAGTCCTTCGCCCAC AGTACATGCTTCTAGTACGCCTGCTGTGAACATTCAGCCAGATGTTTCTGGAGGTTGGGACTGGCATGCT AAACCAGGAGGCTTTGGAATGGGAAGCAAGTCAGCTGCCACCAGCCCAACCGGATCCTCGCATGGTACTC CCACCCATCAAAGCAAACCCCAGACTCTGGATCCTTTTGCCGACCTTGGGACACTAGGTAGTTCTTCCTT TGCCAGCAAACCCACCACACCAACTGGATTGGGTGGAGGATTCCCGCCTCTCAGCTCGCCACAGAAGGCG TCTCCCCAGCCTATGGGTGGCGGGTGGCAGCAGGGAGGTGCCTACAACTGGCAGCAGCCACAGCCTAAGC CTCAGCCCAGCATGCCCCACTCCTCTCCCCAGAACCGACCCAACTACAACGTGAGCTTCTCAGCCATGCC TGGGGGCCAGAACGAACGTGGGAAAGGATCAAGTAATTTGGAAGGGAAACAAAAAGCAGCTGATTTTGAA GACCTACTCTCTGGTCAAGGTTTCAATGCTCACAAAGACAAAAAGGGGCCTCGGACAATAGCTGAGATGA GAAAGGAGGAAATGGCCAAGGAAATGGATCCTGAGAAATTAAAGATTCTGGAATGGATTGAAGGCAAAGA AAGAAATATCAGAGCCCTTCTTTCCACGATGCATACCGTACTATGGGCTGGGGAGACCAAGTGGAAACCA GTTGGCATGGCAGACCTGGTAACACCAGAGCAGGTGAAGAAGGTGTACAGGAAGGCTGTCCTGGTGGTGC ACCCAGATAAAGCTACTGGGCAACCCTATGAACAATACGCAAAGATGATTTTCATGGAGCTCAATGATGC CTGGTCTGAATTTGAAAACCAAGGCCAAAAGCCCTTATAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC235005 representing NM_001256865
Red=Cloning site Green=Tags(s) MEPSYGGGLFDMVKGGAGRLFSNLKDNLKDTLKDTSSRVIQSVTSYTKGDLDFTYVTSRIIVMSFPLDNV DIGFRNQVDDIRSFLDSRHLDHYTVYNLSPKSYRTAKFHSRVSECSWPIRQAPSLHNLFAVCRNMYNWLL QNPKNVCVVHCLDGRAASSILVGAMFIFCNLYSTPGPAIRLLYAKRPGIGLSPSHRRYLGYMCDLLADKP YRPHFKPLTIKSITVSPIPFFNKQRNGCRPYCDVLIGETKIYSTCTDFERMKEYRVQDGKIFIPLNITVQ GDVVVSMYHLRSTIGSRLQAKVTNTQIFQLQFHTGFIPLDTTVLKFTKPELDACDVPEKYPQLFQVTLDV ELQPHDKVIDLTPPWEHYCTKDVNPSILFSSHQEHQDTLALGGQAPIDIPPDNPRHYGQSGFFASLCWQD QKSEKSFCEEDHAALVNQESEQSDDELLTLSSPHGNANGDKPHGVKKPSKKQQEPAAPPPPEDVDLLGLE GSAMSNSFSPPAAPPTNSELLSDLFGGGGAAGPTQAGQSGVEDVFHPSGPASTQSTPRRSATSTSASPTL RVGEGATFDPFGAPSKPSGQDLLGSFLNTSSASSDPFLQPTRSPSPTVHASSTPAVNIQPDVSGGWDWHA KPGGFGMGSKSAATSPTGSSHGTPTHQSKPQTLDPFADLGTLGSSSFASKPTTPTGLGGGFPPLSSPQKA SPQPMGGGWQQGGAYNWQQPQPKPQPSMPHSSPQNRPNYNVSFSAMPGGQNERGKGSSNLEGKQKAADFE DLLSGQGFNAHKDKKGPRTIAEMRKEEMAKEMDPEKLKILEWIEGKERNIRALLSTMHTVLWAGETKWKP VGMADLVTPEQVKKVYRKAVLVVHPDKATGQPYEQYAKMIFMELNDAWSEFENQGQKPLY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001256865 |
ORF Size | 2700 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001256865.1, NP_001243794.1 |
RefSeq Size | 5916 bp |
RefSeq ORF | 2703 bp |
Locus ID | 9829 |
UniProt ID | O75061 |
Cytogenetics | 1p31.3 |
Protein Families | Druggable Genome |
Protein Pathways | Endocytosis |
MW | 99.1 kDa |
Gene Summary | DNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM, Mar 2008] |
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