CNOT10 (NM_001256742) Human Tagged ORF Clone

CAT#: RC234907

CNOT10 (Myc-DDK tagged) - Homo sapiens CCR4-NOT transcription complex, subunit 10 (CNOT10), transcript variant 3

Product Images

Circular map for RC234907

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CNOT10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234907 representing NM_001256742
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTCTATTGGTGTAAAGACTGTCAAGGTTTGTGGGTCAAAGAGCTCTGTACGCATAGAAGGTCAGC
  CATGTTCATTAGGCCAGAGTAGAAGGAAAGTCAAGCTTATGGGTGAACGGTCGTATACTCTTTCAATTGG
  CAACGGAGACTACTTTTGGGCAAATAAAGAAATGCTGTGGGATTATGTGCAGACTCTGTCTGATCAGGGA
  GCAGAGAAACATGAAGGCACAGGTCAGTCCTCTGGGATCACTGATCAAGAGAAGGAGTTATCCACCAATG
  CTTTCCAAGCTTTCACATCTGGAAATTATGATGCCTGTCTACAACACCTTGCCTGTCTACAAGATATAAA
  CAAAGATGATTATAAAATAATTTTGAATACAGCAGTAGCTGAGTTTTTTAAAAGTAACCAAACAACAACA
  GATAATTTGAGACAAACACTTAACCAGCTGAAGAATCAGGTCCACTCAGCTGTTGAAGAAATGGATGGAT
  TAGATGATGTTGAAAACAGCATGTTGTACTATAATCAAGCAGTCATTCTTTATCATCTGCGGCAGTATAC
  AGAAGCCATATCAGTTGGTGAAAAACTTTATCAGTTCATAGAGCCTTTTGAAGAAAAATTTGCCCAAGCA
  GTGTGTTTTTTGCTTGTAGACCTGTATATATTAACCTACCAAGCTGAGAAAGCTTTGCATCTTCTTGCTG
  TCCTAGAAAAAATGATTTCACAGGGTAACAATAACAAAAATGGAAAGAATGAGACTGGTAATAACAACAA
  CAAAGATGGATCTAATCATAAAGCTGAAAGTGGAGCTCTAATAGAAGCTGCAAAATCAAAGATACATCAG
  TACAAAGTACGAGCTTATATCCAAATGAAGTCTCTGAAAGCATGTAAAAGGGAAATCAAGTCAGTCATGA
  ATACAGCTGGAAATTCCGCACCCTCTCTCTTTCTTAAAAGCAATTTTGAGTACTTAAGAGGTAATTATCG
  AAAAGCCGTGAAGCTATTAAATAGTTCAAACATTGCTGAGCATCCAGGATTCATGAAAACAGGTGAATGC
  TTGAGATGCATGTTCTGGAATAACCTTGGTTGCATCCATTTTGCCATGAGCAAGCACAATTTGGGAATAT
  TCTACTTTAAAAAGGCTCTGCAAGAGAATGACAATGTCTGTGCACAGCTCAGTGCAGGTAGCACTGATCC
  AGGTAAAAAATTTTCAGGAAGACCCATGTGTACGTTACTAACCAATAAGAGATATGAGTTGCTGTATAAC
  TGTGGAATTCAGCTTCTTCACATTGGAAGGCCTCTTGCTGCCTTCGAATGTCTGATTGAAGCTGTTCAGG
  TTTATCATGCAAATCCTCGCCTCTGGCTACGGCTGGCTGAATGCTGCATTGCTGCCAATAAGGGGACTTC
  TGAACAAGAAACTAAAGGCCTTCCCAGCAAAAAAGGAATTGTACAGTCTATTGTTGGTCAAGGCTATCAT
  CGTAAAATAGTTTTGGCATCACAGTCTATACAGAATACTGTTTATAATGATGGGCAGTCTTCGGCCATTC
  CTGTAGCCAGTATGGAGTTTGCAGCCATATGTCTCAGAAATGCCTTGTTGCTGCTACCTGAAGAACAGCA
  AGATCCAAAGCAGGAAAATGGGGCTAAAAATAGTAATCAATTAGGTGGGAACACAGAGAGCAGCGAAAGC
  AGTGAAACTTGCAGCAGTAAAAGCCATGATGGAGATAAATTCATTCCAGCTCCACCTTCTTCTCCATTGA
  GAAAACAGGAATTAGAAAACTTAAAGTGCTCCATACTTGCTTGCAGTGCCTACGTGGCTCTGGCTTTGGG
  TGATAACCTCATGGCTTTGAATCATGCAGATAAACTTCTTCAGCAGCCCAAGCTGTCAGGATCTCTTAAG
  TTTTTGGGACATTTATATGCTGCAGAAGCCCTCATCTCTCTCGACAGAATATCTGATGCCATTACTCACT
  TGAACCCGGAGAATGTCACTGATGTCTCCTTAGGGATCTCTTCAAATGAGCAGGACCAAGGATCAGACAA
  AGGTGAAAATGAAGCAATGGAATCCTCTGGTAAGCGGGCCCCTCAGTGCTACCCCAGTTCCGTCAACTCT
  GCCAGGACTGTGATGCTGTTCAACCTTGGCAGCGCTTACTGCCTGAGGAGCGAATATGACAAAGCCCGAA
  AGTGTCTCCACCAGGCGGCTTCAATGATCCATCCTAAAGAGGTGCCCCCTGAGGCCATCTTGCTGGCAGT
  CTACCTTGAACTGCAGAATGGTAATACTCAGCTGGCCTTACAGATCATCAAAAGGAATCAGCTGCTCCCT
  GCAGTGAAAACACACTCTGAAGTGAGAAAGAAGCCAGTGTTTCAGCCTGTCCACCCGATCCAGCCCATCC
  AAATGCCGGCTTTCACCACTGTGCAGAGAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234907 representing NM_001256742
  Red=Cloning site Green=Tags(s)
  
  MKSIGVKTVKVCGSKSSVRIEGQPCSLGQSRRKVKLMGERSYTLSIGNGDYFWANKEMLWDYVQTLSDQG
  AEKHEGTGQSSGITDQEKELSTNAFQAFTSGNYDACLQHLACLQDINKDDYKIILNTAVAEFFKSNQTTT
  DNLRQTLNQLKNQVHSAVEEMDGLDDVENSMLYYNQAVILYHLRQYTEAISVGEKLYQFIEPFEEKFAQA
  VCFLLVDLYILTYQAEKALHLLAVLEKMISQGNNNKNGKNETGNNNNKDGSNHKAESGALIEAAKSKIHQ
  YKVRAYIQMKSLKACKREIKSVMNTAGNSAPSLFLKSNFEYLRGNYRKAVKLLNSSNIAEHPGFMKTGEC
  LRCMFWNNLGCIHFAMSKHNLGIFYFKKALQENDNVCAQLSAGSTDPGKKFSGRPMCTLLTNKRYELLYN
  CGIQLLHIGRPLAAFECLIEAVQVYHANPRLWLRLAECCIAANKGTSEQETKGLPSKKGIVQSIVGQGYH
  RKIVLASQSIQNTVYNDGQSSAIPVASMEFAAICLRNALLLLPEEQQDPKQENGAKNSNQLGGNTESSES
  SETCSSKSHDGDKFIPAPPSSPLRKQELENLKCSILACSAYVALALGDNLMALNHADKLLQQPKLSGSLK
  FLGHLYAAEALISLDRISDAITHLNPENVTDVSLGISSNEQDQGSDKGENEAMESSGKRAPQCYPSSVNS
  ARTVMLFNLGSAYCLRSEYDKARKCLHQAASMIHPKEVPPEAILLAVYLELQNGNTQLALQIIKRNQLLP
  AVKTHSEVRKKPVFQPVHPIQPIQMPAFTTVQRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256742
• ORF Size: 2412 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256742.1, NP_001243671.1
• RefSeq Size: 2778 bp
• RefSeq ORF: 2415 bp
• Locus ID: 25904
• UniProt ID: Q9H9A5
• Cytogenetics: 3p22.3
• Protein Pathways: RNA degradation
• MW: 89.6 kDa
• Gene Summary: Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Is not required for association of CNOT7 to the CCR4-NOT complex.[UniProtKB/Swiss-Prot Function]