ZNF43 (NM_001256650) Human Tagged ORF Clone

CAT#: RC234905

ZNF43 (Myc-DDK tagged) - Homo sapiens zinc finger protein 43 (ZNF43), transcript variant 3

Product Images

Circular map for RC234905

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF43
• Synonyms: HTF6; KOX27; ZNF39L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234905 representing NM_001256650
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTGGCCATAGAATTCTGTCTGGAGGAGTGGCAATGCCTGGACATTGCACAGCAGAATTTATATA
  GGAATGTGATGTTAGAGAACTACAGAAACCTGGTCTTCCTGGGTATTGCTGTCTCTAAGCCAGACCTGAT
  CACCTGTCTGGAGCAAGAAAAAGAGCCTTGGGAGCCTATGAGGAGACATGAAATGGTAGCCAAACCCCCA
  GTTATGTGTTCTCATTTTACCCAAGACTTTTGGCCAGAGCAGCATATAAAAGATCCTTTCCAAAAAGCGA
  CACTGAGAAGATATAAAAACTGTGAACATAAAAATGTACATTTAAAAAAAGACCATAAAAGTGTGGATGA
  GTGTAAGGTGCACAGAGGAGGTTATAATGGATTTAACCAATGTTTGCCAGCTACCCAGAGCAAAATATTT
  CTATTTGATAAATGTGTGAAAGCCTTTCATAAATTTTCAAATTCAAACAGACATAAGATAAGCCATACTG
  AAAAAAAACTTTTCAAATGCAAAGAATGTGGCAAATCATTTTGCATGCTTCCACATCTAGCTCAACATAA
  AATAATTCATACCAGAGTGAATTTCTGCAAATGTGAAAAATGTGGAAAAGCTTTTAACTGCCCTTCAATC
  ATCACTAAACATAAGAGAATTAATACTGGAGAGAAACCCTACACATGTGAAGAATGTGGCAAAGTCTTTA
  ATTGGTCCTCACGCCTTACTACACATAAAAAAAATTATACTAGATACAAACTCTACAAATGTGAAGAATG
  TGGCAAAGCTTTTAACAAGTCCTCAATCCTTACTACCCATAAGATAATTCGCACTGGAGAGAAATTCTAC
  AAATGTAAAGAATGTGCCAAAGCTTTTAACCAATCCTCAAACCTTACTGAACATAAGAAAATTCATCCTG
  GAGAGAAACCTTACAAATGTGAAGAATGTGGCAAAGCCTTTAACTGGCCCTCAACTCTTACTAAACATAA
  GAGAATTCATACTGGAGAGAAACCCTACACATGTGAAGAATGTGGCAAAGCCTTTAACCAGTTCTCAAAC
  CTTACTACACATAAGAGAATCCATACTGCAGAGAAATTCTATAAATGTACAGAATGTGGTGAAGCTTTTA
  GCCGGTCCTCAAACCTTACTAAACATAAGAAAATTCATACTGAAAAGAAACCCTACAAATGTGAAGAATG
  TGGCAAAGCTTTTAAGTGGTCCTCAAAGCTTACTGAACATAAGTTAACTCATACTGGAGAGAAACCCTAC
  AAATGTGAAGAATGTGGCAAAGCCTTTAACTGGCCCTCAACCCTTACTAAACATAACAGAATTCATACTG
  GAGAGAAACCCTACAAATGTGAAGTATGTGGCAAAGCCTTTAACCAGTTCTCAAACCTTACTACACATAA
  GAGAATTCATACTGCAGAAAAACCGTACAAATGTGAAGAATGTGGCAAAGCTTTTAGCCGGTCCTCAAAC
  CTTACTAAACATAAGAAAATTCACATTGAAAAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCTTTTA
  AGTGGTCCTCAAAGCTTACTGAACATAAGATAACTCATACTGGAGAGAAACCCTACAAATGTGAAGAATG
  TGGCAAAGCTTTTAACCATTTCTCAATCCTTACCAAACATAAGAGGATTCATACTGGAGAGAAACCCTAC
  AAGTGTGAAGAATGTGGCAAAGCTTTTACCCAATCCTCAAACCTTACTACACATAAGAAAATTCATACTG
  GAGAGAAATTCTACAAATGTGAAGAATGTGGCAAAGCTTTTACCCAATCTTCAAACCTTACTACACATAA
  AAAAATTCATACTGGAGGAAAACCCTACAAATGTGAAGAATGTGGCAAAGCTTTTAACCAGTTCTCAACT
  CTTACTAAACATAAGATAATTCACACTGAGGAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCCTTTA
  AGTGGTCCTCAACCCTTACTAAACATAAGATAATTCATACTGGAGAGAAACCCTACAAATGTGAAGAATG
  TGGCAAAGCTTTTAAACTGTCCTCAACCCTTTCTACACATAAGATTATTCATACTGGAGAGAAACCCTAC
  AAATGTGAAAAATGTGGCAAAGCTTTTAACCGATCCTCAAACCTTATTGAACATAAGAAAATTCATACTG
  GAGAGCAACCCTACAAATGTGAAGAATGTGGCAAAGCATTTAACTATTCCTCACACCTTAATACACATAA
  GAGAATTCATACTAAAGAGCAACCCTACAAATGTAAAGAATGTGGCAAAGCTTTCAACCAATATTCAAAC
  CTTACTACACATAACAAAATTCATACTGGAGAGAAACTCTACAAACCTGAAGATGTGACAGTGATTTTGA
  CAACACCTCAAACTTTTTCAAACATAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234905 representing NM_001256650
  Red=Cloning site Green=Tags(s)
  
  MDVAIEFCLEEWQCLDIAQQNLYRNVMLENYRNLVFLGIAVSKPDLITCLEQEKEPWEPMRRHEMVAKPP
  VMCSHFTQDFWPEQHIKDPFQKATLRRYKNCEHKNVHLKKDHKSVDECKVHRGGYNGFNQCLPATQSKIF
  LFDKCVKAFHKFSNSNRHKISHTEKKLFKCKECGKSFCMLPHLAQHKIIHTRVNFCKCEKCGKAFNCPSI
  ITKHKRINTGEKPYTCEECGKVFNWSSRLTTHKKNYTRYKLYKCEECGKAFNKSSILTTHKIIRTGEKFY
  KCKECAKAFNQSSNLTEHKKIHPGEKPYKCEECGKAFNWPSTLTKHKRIHTGEKPYTCEECGKAFNQFSN
  LTTHKRIHTAEKFYKCTECGEAFSRSSNLTKHKKIHTEKKPYKCEECGKAFKWSSKLTEHKLTHTGEKPY
  KCEECGKAFNWPSTLTKHNRIHTGEKPYKCEVCGKAFNQFSNLTTHKRIHTAEKPYKCEECGKAFSRSSN
  LTKHKKIHIEKKPYKCEECGKAFKWSSKLTEHKITHTGEKPYKCEECGKAFNHFSILTKHKRIHTGEKPY
  KCEECGKAFTQSSNLTTHKKIHTGEKFYKCEECGKAFTQSSNLTTHKKIHTGGKPYKCEECGKAFNQFST
  LTKHKIIHTEEKPYKCEECGKAFKWSSTLTKHKIIHTGEKPYKCEECGKAFKLSSTLSTHKIIHTGEKPY
  KCEKCGKAFNRSSNLIEHKKIHTGEQPYKCEECGKAFNYSSHLNTHKRIHTKEQPYKCKECGKAFNQYSN
  LTTHNKIHTGEKLYKPEDVTVILTTPQTFSNIK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256650
• ORF Size: 2409 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256650.2
• RefSeq Size: 5508 bp
• RefSeq ORF: 2412 bp
• Locus ID: 7594
• UniProt ID: P17038
• Cytogenetics: 19p12
• Protein Families: Transcription Factors
• MW: 93.9 kDa
• Gene Summary: This gene belongs to the C2H2-type zinc finger gene family. The zinc finger proteins are involved in gene regulation and development, and are quite conserved throughout evolution. Like this gene product, a third of the zinc finger proteins containing C2H2 fingers also contain the KRAB domain, which has been found to be involved in protein-protein interactions. [provided by RefSeq, Jul 2008]