EPB41L1 (NM_001258330) Human Tagged ORF Clone
CAT#: RC234742
- TrueORF®
EPB41L1 (Myc-DDK tagged) - Homo sapiens erythrocyte membrane protein band 4.1-like 1 (EPB41L1), transcript variant 4
ORF Plasmid: tGFP
"NM_001258330" in other vectors (2)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | EPB41L1 |
Synonyms | 4.1N; MRD11 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC234742 representing NM_001258330
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTTTCTTGGGGAGGATCAATGAGGTAGAACCTGCAAAGGGCTTAGCAGAGAGCCTAGCACCTACTG AGCGCTCAGTAAAGAGCCTAGACATGGAGGAGAAGGACTACAGTGAGGCCGATGGCCTTTCGGAGAGGAC CACGCCCAGCAAGGCCCAGAAATCGCCCCAGAAGATTGCCAAGAAATACAAGAGTGCCATCTGCCGGGTC ACTCTGCTTGATGCCTCGGAGTATGAGTGTGAGGTGGAGAAACATGGCCGGGGCCAGGTGCTGTTTGACC TGGTCTGTGAACACCTCAACCTCCTAGAGAAGGACTACTTCGGCCTGACCTTCTGTGATGCTGACAGCCA GAAGAACTGGCTGGACCCCTCCAAGGAGATCAAGAAGCAGATCCGGAGTAGCCCCTGGAATTTTGCCTTC ACAGTCAAGTTCTACCCGCCTGATCCTGCCCAGCTGACAGAAGACATCACAAGATACTACCTGTGCCTGC AGCTGCGGGCAGACATCATCACGGGCCGGCTGCCATGCTCCTTTGTCACGCATGCCCTACTGGGCTCCTA CGCTGTGCAGGCTGAGCTGGGTGACTATGATGCTGAGGAGCATGTGGGCAACTATGTCAGCGAGCTCCGC TTCGCCCCTAACCAGACCCGGGAGCTGGAGGAGAGGATCATGGAGCTGCATAAGACATATAGGGGGATGA CCCCGGGAGAAGCAGAAATCCACTTCTTAGAGAATGCCAAGAAGCTTTCCATGTACGGAGTAGACCTGCA CCATGCCAAGGACTCTGAGGGCATCGACATCATGTTAGGCGTTTGTGCCAATGGCCTGCTCATCTACCGG GACCGGCTGAGAATCAACCGCTTTGCCTGGCCCAAGATCCTCAAGATCTCCTACAAGAGGAGTAACTTCT ATATCAAGATCCGGCCTGGGGAGTATGAGCAATTTGAGAGCACAATTGGCTTTAAGCTCCCAAACCACCG GTCAGCCAAGAGACTGTGGAAGGTCTGCATCGAGCATCATACATTCTTCCGGCTGGTGTCCCCTGAGCCC CCACCCAAGGGCTTCCTGGTGATGGGCTCCAAGTTCCGGTACAGTGGGAGGACCCAGGCACAGACTCGCC AGGCCAGCGCCCTCATTGACCGGCCTGCACCCTTCTTTGAGCGTTCTTCCAGCAAACGGTACACCATGTC CCGCAGCCTTGATGGAGCAGAGTTCTCCCGCCCAGCCTCGGTCAGCGAGAACCATGATGCAGGGCCTGAC GGTGACAAGCGGGATGAGGATGGCGAGTCTGGGGGGCAACGGTCAGAGGCTGAGGAGGGAGAGGTCAGGA CTCCAACCAAGATCAAGGAGCTAAAGTTCTTAGACAAGCCAGAAGATGTCTTGCTGAAGCACCAGGCCAG CATCAATGAGCTCAAAAGGACCCTGAAGGAGCCCAACAGCAAACTCATCCACCGGGATCGAGACTGGGAA CGGGAGCGCAGGCTGCCCTCCTCCCCCGCCTCCCCCTCCCCCAAGGGCACCCCTGAGAAAGCCAATGAGC CCGTGAAAACAGAAACCATGACTGTCAGCAGTCTGGCCATTAGAAAGAAGATTGAGCCGGAGGCCGTACT GCAGACCAGAGTCTCCGCTATGGATAACACCCAGCAGGTTGATGGGAGTGCCTCAGTGGGGAGGGAGTTC ATAGCAACCACTCCCTCCATCACCACGGAGACCATATCGACCACCATGGAGAACAGTCTCAAGTCCGGGA AGGGGGCAGCTGCCATGATCCCAGGCCCACAGACGGTGGCCACGGAAATCCGTTCTCTTTCTCCGATCAT CGGGAAAGATGTCCTCACCAGCACCTACGGCGCCACTGCGGAAACCCTCTCAACCTCCACCACCACCCAT GTCACCAAAACTGTGAAAGGAGGGTTTTCTGAGACAAGGATCGAGAAGCGAATCATCATTACTGGGGATG AAGATGTCGATCAAGACCAGGCCCTGGCTTTGGCCATCAAGGAGGCCAAACTGCAGCATCCTGATATGCT GGTAACCAAAGCTGTCGTATACAGAGAAACAGACCCATCCCCAGAGGAGAGGGACAAGAAGCCACAGGAA TCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC234742 representing NM_001258330
Red=Cloning site Green=Tags(s) MVFLGRINEVEPAKGLAESLAPTERSVKSLDMEEKDYSEADGLSERTTPSKAQKSPQKIAKKYKSAICRV TLLDASEYECEVEKHGRGQVLFDLVCEHLNLLEKDYFGLTFCDADSQKNWLDPSKEIKKQIRSSPWNFAF TVKFYPPDPAQLTEDITRYYLCLQLRADIITGRLPCSFVTHALLGSYAVQAELGDYDAEEHVGNYVSELR FAPNQTRELEERIMELHKTYRGMTPGEAEIHFLENAKKLSMYGVDLHHAKDSEGIDIMLGVCANGLLIYR DRLRINRFAWPKILKISYKRSNFYIKIRPGEYEQFESTIGFKLPNHRSAKRLWKVCIEHHTFFRLVSPEP PPKGFLVMGSKFRYSGRTQAQTRQASALIDRPAPFFERSSSKRYTMSRSLDGAEFSRPASVSENHDAGPD GDKRDEDGESGGQRSEAEEGEVRTPTKIKELKFLDKPEDVLLKHQASINELKRTLKEPNSKLIHRDRDWE RERRLPSSPASPSPKGTPEKANEPVKTETMTVSSLAIRKKIEPEAVLQTRVSAMDNTQQVDGSASVGREF IATTPSITTETISTTMENSLKSGKGAAAMIPGPQTVATEIRSLSPIIGKDVLTSTYGATAETLSTSTTTH VTKTVKGGFSETRIEKRIIITGDEDVDQDQALALAIKEAKLQHPDMLVTKAVVYRETDPSPEERDKKPQE S TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001258330 |
ORF Size | 2103 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001258330.1, NP_001245259.1 |
RefSeq Size | 5745 bp |
RefSeq ORF | 2106 bp |
Locus ID | 2036 |
UniProt ID | Q9H4G0 |
Cytogenetics | 20q11.23 |
Protein Families | Druggable Genome |
Protein Pathways | Tight junction |
MW | 79.3 kDa |
Gene Summary | Erythrocyte membrane protein band 4.1 (EPB41) is a multifunctional protein that mediates interactions between the erythrocyte cytoskeleton and the overlying plasma membrane. The encoded protein binds and stabilizes D2 and D3 dopamine receptors at the neuronal plasma membrane. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015] |
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