TOB (TOB1) (NM_001243885) Human Tagged ORF Clone

CAT#: RC233432

TOB1 (Myc-DDK tagged) - Homo sapiens transducer of ERBB2, 1 (TOB1), transcript variant 3

Product Images

Circular map for RC233432

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TOB
• Synonyms: APRO5; APRO6; PIG49; TOB; TROB; TROB1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233432 representing NM_001243885
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCATAAGTGACCCAGCCTCATCAGTGTCCAGCTCTCCATCGCCTCCTTTTGGTCACTCTGCTGCTG
  TAAGCCCTACCTTCATGCCCCGGTCCACTCAGCCTTTAACCTTTACCACTGCCACTTTTGCTGCCACCAA
  GTTCGGCTCTACCAAAATGAAGAATAGTGGCCGTAGCAACAAGGTTGCACGTACTTCTCCCATCAACCTC
  GGCTTGAATGTGAATGACCTCTTGAAGCAGAAAGCCATCTCTTCCTCAATGCACTCTCTGTATGGGCTTG
  GCTTGGGTAGCCAGCAGCAGCCACAGCAACAGCAGCAGCCAGCCCAGCCGCCACCGCCACCACCACCACC
  ACAGCAGCAACAACAGCAGAAAACCTCTGCTCTTTCTCCTAATGCCAAGGAATTTATTTTTCCTAATATG
  CAGGGTCAAGGTAGTAGTACCAATGGAATGTTCCCAGGTGACAGCCCCCTTAACCTCAGTCCTCTCCAGT
  ACAGTAATGCCTTTGATGTGTTTGCAGCCTATGGAGGCCTCAATGAGAAATCTTTTGTAGATGGCTTGAA
  TTTTAGCTTAAATAACATGCAGTATTCTAACCAGCAATTCCAGCCTGTTATGGCTAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233432 representing NM_001243885
  Red=Cloning site Green=Tags(s)
  
  MPISDPASSVSSSPSPPFGHSAAVSPTFMPRSTQPLTFTTATFAATKFGSTKMKNSGRSNKVARTSPINL
  GLNVNDLLKQKAISSSMHSLYGLGLGSQQQPQQQQQPAQPPPPPPPPQQQQQQKTSALSPNAKEFIFPNM
  QGQGSSTNGMFPGDSPLNLSPLQYSNAFDVFAAYGGLNEKSFVDGLNFSLNNMQYSNQQFQPVMAN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001243885
• ORF Size: 618 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243885.2
• RefSeq Size: 2056 bp
• RefSeq ORF: 621 bp
• Locus ID: 10140
• UniProt ID: P50616
• Cytogenetics: 17q21.33
• Protein Families: Druggable Genome
• MW: 22.5 kDa
• Gene Summary: This gene encodes a member of the transducer of erbB-2 /B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The encoded protein may function as a tumor suppressor. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]