WDR51B (POC1B) (NM_001199777) Human Tagged ORF Clone

CAT#: RC232723

POC1B (Myc-DDK tagged) - Homo sapiens POC1 centriolar protein B (POC1B), transcript variant 2

Product Images

Circular map for RC232723

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WDR51B
• Synonyms: CORD20; PIX1; TUWD12; WDR51B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC232723 representing NM_001199777
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTATGGAATTTCAAGCCACATGCTAGAGCTTACAGATATGTGGGTCACAAGGATGTTGTAACCAGCG
  TGCAGTTTTCTCCACATGGAAACTTATTGGCGTCTGCCTCACGAGACAGAACCGTGAGACTCTGGATTCC
  TGATAAGAGAGGAAAATTCTCAGAATTTAAAGCTCATACAGCTCCAGTTCGAAGTGTAGACTTTTCAGCT
  GATGGCCAGTTTCTAGCTACAGCTTCTGAAGACAAATCCATAAAAGTATGGAGCATGTATCGCCAGCGCT
  TCCTGTATTCCTTGTATCGACATACACACTGGGTACGCTGTGCCAAATTTTCACCCGATGGAAGACTAAT
  TGTGTCATGTAGTGAGGATAAAACTATTAAAATTTGGGATACCACAAATAAGCAATGTGTTAATAACTTC
  TCAGATTCCGTTGGATTTGCAAATTTTGTGGACTTTAACCCTAGTGGTACATGCATAGCTTCAGCAGGTT
  CTGATCAAACTGTGAAAGTCTGGGATGTAAGAGTGAACAAATTACTACAGCATTACCAAGTTCACAGCGG
  TGGAGTTAATTGCATATCATTCCATCCTTCGGGTAACTATCTCATCACAGCTTCTTCAGATGGTACCCTT
  AAGATTCTGGACCTCTTAGAAGGAAGGCTCATCTATACACTTCAAGGACATACGGGACCTGTCTTTACTG
  TTTCATTTTCAAAAGGTGGAGAGCTATTTGCATCAGGAGGTGCAGACACACAGGTCTTATTATGGAGGAC
  TAACTTTGATGAATTGCATTGTAAAGGTCTTACCAAAAGAAATCTCAAAAGATTACATTTTGATTCACCA
  CCACATCTTCTTGATATCTACCCAAGAACACCACATCCCCATGAGGAAAAAGTTGAGACTGTAGAAATTA
  ATCCAAAGCTTGAGGTAATCGATTTGCAGATCTCTACTCCCCCTGTTATGGATATCCTTTCTTTTGATTC
  TACCACAACAACAGAAACCAGTGGTAGGACTCTGCCAGACAAGGGTGAAGAGGCCTGTGGATATTTCTTG
  AACCCTTCCTTAATGTCACCAGAATGTTTGCCAACAACCACGAAAAAGAAAACAGAAGACATGAGTGACC
  TCCCCTGTGAAAGTCAAAGGAGCATACCTCTCGCTGTGACTGATGCTTTAGAGCATATTATGGAACAACT
  CAATGTTTTGACACAGACTGTTTCAATCTTGGAGCAGCGACTGACTTTGACAGAGGATAAGCTGAAAGAC
  TGCCTTGAAAATCAGCAAAAGCTTTTCAGTGCTGTCCAACAGAAAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC232723 representing NM_001199777
  Red=Cloning site Green=Tags(s)
  
  MLWNFKPHARAYRYVGHKDVVTSVQFSPHGNLLASASRDRTVRLWIPDKRGKFSEFKAHTAPVRSVDFSA
  DGQFLATASEDKSIKVWSMYRQRFLYSLYRHTHWVRCAKFSPDGRLIVSCSEDKTIKIWDTTNKQCVNNF
  SDSVGFANFVDFNPSGTCIASAGSDQTVKVWDVRVNKLLQHYQVHSGGVNCISFHPSGNYLITASSDGTL
  KILDLLEGRLIYTLQGHTGPVFTVSFSKGGELFASGGADTQVLLWRTNFDELHCKGLTKRNLKRLHFDSP
  PHLLDIYPRTPHPHEEKVETVEINPKLEVIDLQISTPPVMDILSFDSTTTTETSGRTLPDKGEEACGYFL
  NPSLMSPECLPTTTKKKTEDMSDLPCESQRSIPLAVTDALEHIMEQLNVLTQTVSILEQRLTLTEDKLKD
  CLENQQKLFSAVQQKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199777
• ORF Size: 1308 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199777.2
• RefSeq Size: 3232 bp
• RefSeq ORF: 1311 bp
• Locus ID: 282809
• UniProt ID: Q8TC44
• Cytogenetics: 12q21.33
• MW: 49.6 kDa
• Gene Summary: POC1 proteins contain an N-terminal WD40 domain and a C-terminal coiled coil domain and are part of centrosomes. They play an important role in basal body and cilia formation. This gene encodes one of the two POC1 proteins found in humans. Mutation in this gene result in autosomal-recessive cone-rod dystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]