CDK15 (NM_001261436) Human Tagged ORF Clone

CAT#: RC232657

CDK15 (Myc-DDK tagged) - Homo sapiens cyclin-dependent kinase 15 (CDK15), transcript variant 2

Product Images

Circular map for RC232657

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDK15
• Synonyms: ALS2CR7; PFTAIRE2; PFTK2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC232657 representing NM_001261436
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCAAGAGCTGTGTGCAAAGACTGTACAGCCTGGATGCAGCTGCTACCATTGTTCAGAGGGAGGCG
  AGGCACACAGCTGTCGGAGGAGTCAGCCTGAGACCACGGAGGCTGCGTTCAAGCTAACAGACCTAAAAGA
  AGCATCATGTTCCATGACTTCATTTCACCCCAGGGGACTTCAAGCTGCCCGTGCCCAGAAGTTCAAGAGT
  AAAAGGCCACGGAGTAACAGTGATTGTTTTCAGGAAGAGGATCTGAGGCAGGGTTTTCAGTGGAGGAAGA
  GCCTCCCTTTTGGGGCAGCCTCATCTTACTTGAACTTGGAGAAGCTGGGTGAAGGCTCTTATGCGACAGT
  TTACAAGGGGATTAGCAGAATAAATGGACAACTAGTGGCTTTAAAAGTCATCAGCATGAATGCAGAGGAA
  GGAGTCCCATTTACAGCTATCCGAGAAGCTTCTCTCCTGAAGGGTTTGAAACATGCCAATATTGTGCTCC
  TGCATGACATAATCCACACCAAAGAGACACTGACATTCGTTTTTGAATACATGCACACAGACCTGGCCCA
  GTATATGTCTCAGCATCCAGGAGGGCTTCATCCTCATAATGTCAGACTTTTCATGTTTCAACTTTTGCGG
  GGCCTGGCGTACATCCACCACCAACACGTTCTTCACAGGGACCTGAAACCTCAGAACTTACTCATCAGTC
  ACCTGGGAGAGCTCAAACTGGCTGATTTTGGTCTTGCCCGGGCCAAGTCCATTCCCAGCCAGACATACTC
  TTCAGAAGTCGTGACCCTCTGGTACCGGCCCCCTGATGCTTTGCTGGGAGCCACTGAATATTCCTCTGAG
  CTGGACATATGGGGTGCAGGCTGCATCTTTATTGAAATGTTCCAGGGTCAACCTTTGTTTCCTGGGGTTT
  CCAACATCCTTGAACAGCTGGAGAAAATCTGGGAGGTGCTGGGAGTCCCTACAGAGGATACTTGGCCGGG
  AGTCTCCAAGCTACCTAACTACAATCCAGAATGGTTCCCACTGCCTACGCCTCGAAGCCTTCATGTTGTC
  TGGAACAGGCTGGGCAGGGTTCCTGAAGCTGAAGACCTGGCCTCCCAGATGCTAAAAGGCTTTCCCAGAG
  ACCGCGTCTCCGCCCAGGAAGCACTTGTTCATGATTATTTCAGCGCCCTGCCATCTCAGCTGTACCAGCT
  TCCTGATGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC232657 representing NM_001261436
  Red=Cloning site Green=Tags(s)
  
  MGQELCAKTVQPGCSCYHCSEGGEAHSCRRSQPETTEAAFKLTDLKEASCSMTSFHPRGLQAARAQKFKS
  KRPRSNSDCFQEEDLRQGFQWRKSLPFGAASSYLNLEKLGEGSYATVYKGISRINGQLVALKVISMNAEE
  GVPFTAIREASLLKGLKHANIVLLHDIIHTKETLTFVFEYMHTDLAQYMSQHPGGLHPHNVRLFMFQLLR
  GLAYIHHQHVLHRDLKPQNLLISHLGELKLADFGLARAKSIPSQTYSSEVVTLWYRPPDALLGATEYSSE
  LDIWGAGCIFIEMFQGQPLFPGVSNILEQLEKIWEVLGVPTEDTWPGVSKLPNYNPEWFPLPTPRSLHVV
  WNRLGRVPEAEDLASQMLKGFPRDRVSAQEALVHDYFSALPSQLYQLPDE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001261436
• ORF Size: 1200 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001261436.1, NP_001248365.1
• RefSeq Size: 2292 bp
• RefSeq ORF: 1203 bp
• Locus ID: 65061
• UniProt ID: Q96Q40
• Cytogenetics: 2q33.1
• Protein Families: Druggable Genome, Protein Kinase
• MW: 45.5 kDa
• Gene Summary: Serine/threonine-protein kinase that acts like an antiapoptotic protein that counters TRAIL/TNFSF10-induced apoptosis by inducing phosphorylation of BIRC5 at 'Thr-34'.[UniProtKB/Swiss-Prot Function]