TMTC1 (NM_001193451) Human Tagged ORF Clone

CAT#: RC231432

TMTC1 (Myc-DDK-tagged)-Human transmembrane and tetratricopeptide repeat containing 1 (TMTC1), transcript variant 1

Product Images

Circular map for RC231432

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMTC1
• Synonyms: ARG99; OLF; TMTC1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231432 representing NM_001193451
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGTGACCACCTCTGCCCGAGGCGGCGGCGGGGACCGCACACCCTCCCGGCGGCGGGGCTGCGGGC
  TAGCGCCGGCCGGGGCCGCGGCGCTGCTGGCCGGGGCAAGCTGCCTGTGCTACGGCCGCTCCCTGCAGGG
  CGAGTTCGTGCACGACGACGTGTGGGCGATCGTGAACAACCCCGACGTGCGGCCCGGCGCCCCGCTCCGC
  TGGGGCATCTTCACCAACGACTTCTGGGGCAAGGGCATGGCCGAGAACACCAGCCACAAGTCCTACCGGC
  CGCTCTGCGTCCTCACCTTCAAGCTAAACATATTTTTGACTGGTATGAACCCATTCTACTTTCATGCAGT
  AAATATAATTTTACACTGCTTAGTGACTCTTGTGCTGATGTACACCTGTGATAAAACTGTCTTCAAGAAT
  CGTGGACTTGCTTTTGTAACGGCATTGCTTTTTGCTGTACATCCTATTCATACTGAGGCGGTGGCTGGGA
  TCGTTGGCAGAGCGGACGTGTTAGCGTGTCTGCTGTTTCTATTGGCCTTTCTCTCGTACAACAGGAGTCT
  GGATCAGGGCTGTGTTGGGGGAAGTTTCCCTTCCACGGTGTCTCCCTTCTTCTTGCTGCTCAGTTTGTTT
  CTGGGGACCTGTGCGATGCTGGTGAAAGAGACAGGCATCACGGTGTTTGGAGTGTGCTTGGTTTATGACC
  TCTTTTCCCTTTCCAACAAGCAAGACAAGTCGAGCAATGGGGCCCTCTGTCCACGCAGCCCACAGCAGCC
  CGGGAGCCCCCAGCCCTCCTCACTGCCAGGCCATCCTCACCGGGAGAATGGGAAGCAGCAGCGGTTCCCT
  CACAAAGGAGCTTGGGGTGGCTGCCACTCTCCACTGCCACCAGAACCCAAGAGCAGTGGATTCCCAGTGT
  CCCCACGAGCTGTGTGGTCCATGATGAGATTCCTCACCTATTCCTACCTCTTGGCCTTCAATGTGTGGCT
  TCTGCTTGCACCCGTGACCCTGTGCTATGACTGGCAGGTCGGCAGTATTCCTCTGGTAGAGACCATATGG
  GACATGCGGAACTTAGCCACCATCTTTCTGGCGGTTGTGATGGCCTTATTGAGCCTGCACTGCTTAGCAG
  CCTTTAAGAGACTGGAGCACAAGGAGGTTTTAGTCGGCTTGTTGTTCCTGGTGTTCCCGTTCATTCCAGC
  CAGCAACCTCTTCTTCAGGGTGGGTTTTGTGGTGGCGGAGAGAGTCCTTTACATGCCTAGCATGGGCTAC
  TGCATCCTTTTTGTGCATGGACTGAGCAAGCTCTGCACTTGGCTGAATCGATGTGGGGCCACCACCCTGA
  TTGTGTCCACTGTGTTGCTGCTGTTGCTTTTCTCTTGGAAAACTGTGAAACAGAATGAAATTTGGCTGTC
  AAGAGAGTCCCTATTCAGGTCTGGAGTTCAAACTCTGCCCCACAATGCCAAGGTTCACTACAACTATGCC
  AATTTCCTGAAGGACCAAGGTCGGAACAAGGAAGCGATCTACCACTACAGAACAGCTCTCAAGTTGTATC
  CACGCCATGCAAGTGCGCTCAACAACCTTGGAACACTGACGAGAGACACAGCAGAGGCAAAGATGTACTA
  TCAGAGGGCTCTCCAGCTCCATCCACAGCATAACCGGGCTCTTTTCAATCTGGGGAATCTCCTCAAGTCC
  CAGGAGAAAAAGGAAGAAGCTATCACCTTACTGAAGGATTCCATCAAATATGGTCCAGAGTTTGCAGATG
  CATATTCAAGCTTAGCTTCGTTATTGGCTGAGCAGGAGCGGTTTAAAGAAGCTGAAGAAATATACCAAAC
  TGGAATAAAGAACTGTCCAGACAGCTCAGATTTACACAACAACTATGGGGTTTTCTTAGTTGATACTGGC
  TTACCAGAAAAGGCAGTGGCCCATTACCAGCAGGCCATCAAACTTAGCCCCAGTCATCACGTGGCCATGG
  TGAACTTGGGAAGACTCTACAGGTCACTGGGAGAGAACAGCATGGCTGAAGAATGGTACAAGCGCGCCCT
  GCAGGTGGCACACAAAGCTGAGATATTGTCACCTTTGGGAGCACTGTATTACAACACTGGCCGATACGAA
  GAGGCTTTGCAGATTTACCAGGAAGCTGCAGCACTTCAGCCTTCTCAGAGGGAGCTCCGCTTGGCACTGG
  CTCAGGTTTTGGCCGTGATGGGTCAGACAAAAGAAGCTGAAAAGATGACCAATCACATTGTGTCAGAGGA
  GACCGGATGCCTTGAATGCTATCGCCTCTTGTCAGCCATCTATAGCAAGCAGGAGAACCACGACAAGGCA
  CTTGATGCTATAGACAAGGCTCTCCAGCTGAAACCAAAGGACCCAAAAGTCATTTCTGAACTTTTTTTCA
  CAAAAGGAAACCAATTAAGAGAGCAGAACCTTCTCGACAAAGCTTTTGAGAGCTATAGAGTGGCTGTGCA
  ACTAAACCCAGACCAAGCACAGGCCTGGATGAACATGGGTGGCATCCAACACATCAAGGGAAAATATGTG
  TCTGCAAGAGCTTATTATGAGAGAGCCTTACAGCTGGTTCCAGACAGCAAACTGCTGAAGGAAAATCTTG
  CCAAATTGGATCGCCTAGAAAAACGATTACAAGAAGTTCGAGAAAAGGATCAAACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231432 representing NM_001193451
  Red=Cloning site Green=Tags(s)
  
  MVVTTSARGGGGDRTPSRRRGCGLAPAGAAALLAGASCLCYGRSLQGEFVHDDVWAIVNNPDVRPGAPLR
  WGIFTNDFWGKGMAENTSHKSYRPLCVLTFKLNIFLTGMNPFYFHAVNIILHCLVTLVLMYTCDKTVFKN
  RGLAFVTALLFAVHPIHTEAVAGIVGRADVLACLLFLLAFLSYNRSLDQGCVGGSFPSTVSPFFLLLSLF
  LGTCAMLVKETGITVFGVCLVYDLFSLSNKQDKSSNGALCPRSPQQPGSPQPSSLPGHPHRENGKQQRFP
  HKGAWGGCHSPLPPEPKSSGFPVSPRAVWSMMRFLTYSYLLAFNVWLLLAPVTLCYDWQVGSIPLVETIW
  DMRNLATIFLAVVMALLSLHCLAAFKRLEHKEVLVGLLFLVFPFIPASNLFFRVGFVVAERVLYMPSMGY
  CILFVHGLSKLCTWLNRCGATTLIVSTVLLLLLFSWKTVKQNEIWLSRESLFRSGVQTLPHNAKVHYNYA
  NFLKDQGRNKEAIYHYRTALKLYPRHASALNNLGTLTRDTAEAKMYYQRALQLHPQHNRALFNLGNLLKS
  QEKKEEAITLLKDSIKYGPEFADAYSSLASLLAEQERFKEAEEIYQTGIKNCPDSSDLHNNYGVFLVDTG
  LPEKAVAHYQQAIKLSPSHHVAMVNLGRLYRSLGENSMAEEWYKRALQVAHKAEILSPLGALYYNTGRYE
  EALQIYQEAAALQPSQRELRLALAQVLAVMGQTKEAEKMTNHIVSEETGCLECYRLLSAIYSKQENHDKA
  LDAIDKALQLKPKDPKVISELFFTKGNQLREQNLLDKAFESYRVAVQLNPDQAQAWMNMGGIQHIKGKYV
  SARAYYERALQLVPDSKLLKENLAKLDRLEKRLQEVREKDQT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001193451
• ORF Size: 2646 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001193451.2
• RefSeq ORF: 2649 bp
• Locus ID: 83857
• UniProt ID: Q8IUR5
• Cytogenetics: 12p11.22
• Protein Families: Transmembrane
• MW: 99.3 kDa
• Gene Summary: Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. The 4 members of the TMTC family are O-mannosyl-transferases dedicated primarily to the cadherin superfamily, each member seems to have a distinct role in decorating the cadherin domains with O-linked mannose glycans at specific regions. Also acts as O-mannosyl-transferase on other proteins such as PDIA3.[UniProtKB/Swiss-Prot Function]