KF1 (RNF103) (NM_001198951) Human Tagged ORF Clone

CAT#: RC231371

RNF103 (Myc-DDK-tagged)-Human ring finger protein 103 (RNF103), transcript variant 2

Product Images

Circular map for RC231371

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KF1
• Synonyms: HKF-1; KF-1; KF1; ZFP-103; ZFP103
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231371 representing NM_001198951
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCGGCTCCGGCCGCGGCCGCCGCTGATACACGGTTGTGGAGGGTTTTTTGAGGCCATTGTGTGGT
  ATGAAACTGGCATCTTTGCCACCCAGCTGGTGGATCCGGTGGCGCTGAGCTTCAAGAAGCTGAAGACCAT
  TTTGGAGTGCCGGGGGTTGGGCTACTCAGGGTTGCCCGAGAAGAAGGATGTCCGGGAGCTGGTGGAAAAG
  TCAGGTGACTTGATGGAGGGTGAGCTCTATTCTGCTCTCAAGGAAGAAGAAGCATCCGAATCGGTTTCTA
  GTACCAATTTCAGTGGTGAAATGCACTTCTATGAGCTTGTGGAAGACACAAAAGATGGCATCTGGCTGGT
  TCAGGTCATAGCAAATGACAGAAGTCCCTTGGTGGGCAAAATTCACTGGGAGAAAATGGTTAAAAAGGTG
  TCAAGATTTGGAATACGTACAGGCACATTTAACTGTTCCAGTGATCCCAGATATTGCAGGAGAAGAGGCT
  GGGTCCGATCCACACTCATTATGTCTGTTCCACAAACAAGTACTTCAAAAGGGAAAGTCATGCTTAAAGA
  ATACAGTGGACGCAAGATTGAAGTAGAGCACATTTTTAAATGGATAACTGCTCATGCAGCTTCTCGGATC
  AAAACCATTTATAATGCTGAACACTTGAAAGAAGAATGGAATAAAAGTGATCAGTATTGGTTAAAAATAT
  ACCTATTTGCAAACCTTGACCAGCCCCCAGCTTTCTTCTCTGCACTAAGTATAAAGTTTACTGGAAGAGT
  TGAGTTTATTTTTGTTAATGTAGAAAATTGGGACAACAAGAGTTATATGACAGATATTGGCATATATAAT
  ATGCCATCATACATACTTAGAACTCCTGAAGGAATTTACAGGTATGGAAACCACACAGGCGAATTTATAT
  CCCTTCAGGCCATGGATTCATTTTTGCGCTCATTACAACCCGAGGTAAATGATCTGTTTGTTTTGAGCTT
  GGTTCTAGTTAATCTTATGGCTTGGATGGACTTATTTATTACACAAGGAGCTACCATAAAGCGATTTGTG
  GTTCTCATAAGCACTTTAGGGACATATAATTCTCTATTAATTATTTCCTGGCTACCTGTGTTGGGCTTTT
  TACAGCTACCTTACTTAGATAGCTTTTATGAATATAGCTTAAAATTGTTGAGATATTCCAATACAACCAC
  ACTGGCTTCATGGGTAAGGGCAGACTGGATGTTTTACTCTTCACACCCAGCCCTGTTTCTCAGTACATAC
  CTTGGTCATGGTTTACTAATTGATTACTTTGAGAAGAAGAGAAGGCGCAACAACAACAATGATGAAGTCA
  ATGCCAATAACTTAGAATGGTTATCAAGTCTGTGGGACTGGTACACCAGCTACCTCTTCCACCCGATTGC
  TTCTTTTCAGAACTTTCCTGTAGAATCTGATTGGGACGAAGACCCTGACTTATTCTTGGAGCGCTTAGCT
  TTCCCTGACCTTTGGCTTCACCCTCTGATACCAACTGATTATATTAAAAACTTACCAATGTGGCGATTTA
  AATGTCTTGGAGTCCAGTCTGAAGAGGAAATGTCGGAGGGGTCTCAAGATACTGAAAATGACTCGGAAAG
  TGAGAACACAGACACTTTGAGTAGTGAGAAGGAAGTATTTGAAGATAAGCAAAGCGTACTTCACAATTCT
  CCAGGAACAGCAAGTCACTGTGATGCTGAGGCTTGTTCATGTGCCAATAAATATTGTCAGACCAGCCCAT
  GTGAAAGGAAGGGGAGGTCATATGGATCATATAACACTAATGAAGATATGGAACCTGATTGGTTAACTTG
  GCCTGCTGATATGCTGCACTGTACTGAATGTGTTGTTTGCCTAGAGAATTTTGAAAATGGATGTTTGCTA
  ATGGGGTTGCCTTGTGGTCATGTGTTTCATCAGAATTGCATTGTGATGTGGTTGGCTGGGGGCCGACATT
  GTTGCCCTGTTTGCCGGTGGCCTTCTTATAAAAAAAAGCAGCCATATGCACAACACCAGCCCTTGTCAAA
  TGATGTCCCATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231371 representing NM_001198951
  Red=Cloning site Green=Tags(s)
  
  MSRLRPRPPLIHGCGGFFEAIVWYETGIFATQLVDPVALSFKKLKTILECRGLGYSGLPEKKDVRELVEK
  SGDLMEGELYSALKEEEASESVSSTNFSGEMHFYELVEDTKDGIWLVQVIANDRSPLVGKIHWEKMVKKV
  SRFGIRTGTFNCSSDPRYCRRRGWVRSTLIMSVPQTSTSKGKVMLKEYSGRKIEVEHIFKWITAHAASRI
  KTIYNAEHLKEEWNKSDQYWLKIYLFANLDQPPAFFSALSIKFTGRVEFIFVNVENWDNKSYMTDIGIYN
  MPSYILRTPEGIYRYGNHTGEFISLQAMDSFLRSLQPEVNDLFVLSLVLVNLMAWMDLFITQGATIKRFV
  VLISTLGTYNSLLIISWLPVLGFLQLPYLDSFYEYSLKLLRYSNTTTLASWVRADWMFYSSHPALFLSTY
  LGHGLLIDYFEKKRRRNNNNDEVNANNLEWLSSLWDWYTSYLFHPIASFQNFPVESDWDEDPDLFLERLA
  FPDLWLHPLIPTDYIKNLPMWRFKCLGVQSEEEMSEGSQDTENDSESENTDTLSSEKEVFEDKQSVLHNS
  PGTASHCDAEACSCANKYCQTSPCERKGRSYGSYNTNEDMEPDWLTWPADMLHCTECVVCLENFENGCLL
  MGLPCGHVFHQNCIVMWLAGGRHCCPVCRWPSYKKKQPYAQHQPLSNDVPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198951
• ORF Size: 2043 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198951.1, NP_001185880.1
• RefSeq ORF: 2046 bp
• Locus ID: 7844
• UniProt ID: O00237
• Cytogenetics: 2p11.2
• Protein Families: Druggable Genome, Transcription Factors, Transmembrane
• MW: 79.1 kDa
• Gene Summary: The protein encoded by this gene contains a RING-H2 finger, a motif known to be involved in protein-protein and protein-DNA interactions. This gene is highly expressed in normal cerebellum, but not in the cerebral cortex. The expression of the rat counterpart in the frontal cortex and hippocampus was shown to be induced by elctroconvulsive treatment (ECT) as well as chronic antidepressant treatment, suggesting that this gene may be a molecular target for ECT and antidepressants. The protein is a ubiquitin ligase that functions in the endoplasmic reticulum-associated degradation pathway. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream CHMP3 (charged multivesicular body protein 3) gene. [provided by RefSeq, Oct 2011]