Myeloid leukemia factor 1 (MLF1) (NM_001195434) Human Tagged ORF Clone
CAT#: RC231106
- TrueORF®
MLF1 (Myc-DDK-tagged)-Human myeloid leukemia factor 1 (MLF1), transcript variant 6
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001195434" in other vectors (3)
USD 198.00
USD 447.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Myeloid leukemia factor 1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC231106 representing NM_001195434
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGACAGATGATAAGAAGTTTTTCTGAACCCTTTGGAAGAGACTTGCTCAGTATCTCTGATGGTAGAG GGAGAGCTCATAATCGTAGAGGACATAATGATGGTGAAGATTCTTTGACTGCAACGAGTTGTTCTCTTGT GCCTTTTGGCGATTTTGGTGGTATGCATACAGATGTCAGCTCTTTCCAGACAATGGACCAAATGGTGTCA AATATGAGAAACTATATGCAGAAATTAGAAAGAAACTTCGGTCAACTTTCAGTGGATCCAAATGGACATT CATTTTGTTCTTCCTCAGTTATGACTTATTCCAAAATAGGAGATGAACCGCCAAAGGTTTTTCAGGCCTC AACTCAAACTCGTCGAGCTCCAGGAGGAATAAAGGAAACCAGGAAAGCAATGAGAGATTCTGACAGTGGA CTAGAAAAAATGGCTATTGGTCATCATATCCATGACCGAGCTCATGTCATTAAAAAGTCAAAGAACAAGA AGACTGGAGATGAAGAGGTCAACCAGGAGTTCATCAATATGAATGAAAGTGATGCTCATGCTTTTGATGA GGAGTGGCAAAGTGAGGTTTTGAAGTACAAACCAGGACGACACAATCTAGGAAACACTAGAATGAGAAGT GTTGGCCATGAGAATCCTGGCTCCCGAGAACTTAAAAGAAGGGAGAAACCTCAACAAAGTCCAGCCATTG AACATGGAAGGAGATCAAATGTTTTGGGGGACAAACTCCACATCAAAGGCTCATCTGTGAAAAGCAACAA AAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC231106 representing NM_001195434
Red=Cloning site Green=Tags(s) MRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTATSCSLVPFGDFGGMHTDVSSFQTMDQMVS NMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSG LEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRS VGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001195434 |
ORF Size | 774 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001195434.2 |
RefSeq ORF | 777 bp |
Locus ID | 4291 |
UniProt ID | P58340 |
Cytogenetics | 3q25.32 |
Protein Families | Druggable Genome |
MW | 29.5 kDa |
Gene Summary | This gene encodes an oncoprotein which is thought to play a role in the phenotypic determination of hemopoetic cells. Translocations between this gene and nucleophosmin have been associated with myelodysplastic syndrome and acute myeloid leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC231106L3 | Lenti-ORF clone of MLF1 (Myc-DDK-tagged)-Human myeloid leukemia factor 1 (MLF1), transcript variant 6 |
USD 630.00 |
|
RC231106L4 | Lenti-ORF clone of MLF1 (mGFP-tagged)-Human myeloid leukemia factor 1 (MLF1), transcript variant 6 |
USD 630.00 |
|
RG231106 | MLF1 (tGFP-tagged) - Human myeloid leukemia factor 1 (MLF1), transcript variant 6 |
USD 530.00 |
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