ANKRD50 (NM_001167882) Human Tagged ORF Clone

CAT#: RC230682

ANKRD50 (Myc-DDK-tagged)-Human ankyrin repeat domain 50 (ANKRD50), transcript variant 2

Product Images

Circular map for RC230682

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANKRD50
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230682 representing NM_001167882
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCCTCCCCAGCAAAGCCTATACCTGCTTGTTGATTCTGTTGATGAAGGGTGTAACATTACTGAAG
  GTGAACAAACGTCTACCAGCTTATCTGGGACTGTTGCAGCACTTTTAGCTGGTCACCATGAGTTCTTTCC
  ACCATGGCTATTGCTTCTCTGTTCTGCCCGAAAGCAGAGTAAGGCTGTTACTAAAATGTTTACTGGTTTT
  CGAAAAATAAGTTTAGATGACCTTCGGAAGGCATATATCGTCAAGGATGTTCAGCAGTACATTCTTCATC
  GTTTAGATCAAGAAGAAGCTTTGCGACAACACCTCACAAAAGAAACTGCAGAGATGTTAAATCAACTGCA
  CATTAAAAGCAGTGGATGCTTTCTTTACCTAGAACGAGTTTTAGATGGAGTTGTAGAAAATTTTATTATG
  TTAAGAGAAATTCGTGACATCCCAGGAACTCTAAATGGTTTATATCTCTGGCTGTGCCAAAGACTTTTTG
  TAAGAAAACAATTTGCAAAGGTTCAGCCTATTTTGAATGTGATTCTTGCAGCCTGCCGACCTTTGACCAT
  AACGGAATTATATCACGCAGTATGGACCAAAAACATGTCGTTAACTTTGGAAGATTTTCAACGCAAGTTA
  GATATCCTCTCCAAACTTCTTGTTGATGGACTAGGAAATACAAAAATACTGTTTCATTATAGTTTTGCCG
  AGTGGCTTCTGGATGTGAAACACTGTACTCAGAAGTATTTATGTAATGCAGCAGAAGGACACAGAATGTT
  GGCTATGAGTTATACCTGTCAAGCCAAGAATTTAACACCATTGGAAGCACAAGAATTTGCATTGCACTTA
  ATTAACTCAAACTTACAATTAGAGACAGCGGAGTTAGCTCTGTGGATGATATGGAATGGTACACCTGTCA
  GAGATTCCCTTTCTACTTTGATACCCAAGGAACAAGAAGTGCTACAGCTGTTGGTTAAAGCTGGGGCTCA
  TGTCAACAGTGAAGACGATCGCACATCATGCATAGTTCGACAAGCCTTAGAAAGAGAGGATTCCATTCGG
  ACATTATTAGATAATGGAGCTTCAGTAAATCAGTGTGATTCAAATGGGAGAACATTATTGGCTAATGCTG
  CATATAGTGGCAGTCTTGATGTAGTCAATTTACTTGTCTCTAGGGGAGCAGATTTAGAGATAGAAGATGC
  TCATGGACATACACCACTCACTCTAGCGGCTAGACAGGGACATACCAAGGTGGTTAATTGTTTGATTGGG
  TGTGGAGCAAATATTAATCATACTGATCAAGATGGTTGGACAGCATTAAGATCTGCTGCTTGGGGTGGCC
  ATACTGAGGTAGTTTCTGCACTACTTTATGCTGGCGTAAAAGTGGATTGTGCAGATGCTGATAGCCGAAC
  AGCTTTGAGAGCAGCAGCATGGGGAGGACACGAGGATATTGTACTGAATTTGCTACAACATGGCGCTGAA
  GTGAACAAAGCTGATAATGAAGGTAGAACTGCTTTGATAGCAGCAGCATACATGGGACATAGAGAGATTG
  TGGAACACCTACTGGACCATGGAGCAGAAGTAAATCATGAGGATGTTGATGGCAGGACTGCACTCTCTGT
  AGCTGCACTTTGTGTGCCTGCAAGTAAAGGGCACGCATCAGTTGTTAGCCTTTTAATTGATCGAGGTGCT
  GAAGTAGATCATTGTGATAAAGATGGCATGACTCCACTGCTGGTAGCTGCCTATGAAGGACATGTTGATG
  TGGTTGACTTGCTTCTAGAAGGGGGAGCAGATGTAGATCACACAGATAACAATGGCCGTACACCCCTCTT
  AGCAGCAGCGTCTATGGGTCATGCATCAGTTGTAAATACACTTTTGTTTTGGGGTGCAGCTGTGGATAGT
  ATTGATAGTGAAGGTAGGACAGTCCTCAGTATAGCTTCAGCACAAGGAAATGTTGAGGTGGTACGTACTC
  TACTGGATAGAGGGTTAGATGAAAATCACAGAGATGATGCTGGATGGACACCTTTGCACATGGCAGCTTT
  TGAAGGGCACAGATTGATATGTGAAGCACTTATTGAACAAGGTGCTAGAACAAATGAGATTGACAATGAT
  GGACGAATCCCTTTCATATTAGCTTCACAAGAGGGTCATTATGATTGTGTTCAAATATTACTGGAAAACA
  AATCCAACATTGATCAAAGAGGTTATGATGGAAGAAATGCACTGCGGGTTGCTGCATTAGAAGGGCACAG
  GGACATTGTTGAATTGCTTTTTAGCCATGGTGCTGATGTTAACTGCAAAGATGCTGATGGTCGGCCTACA
  CTTTATATCTTGGCCTTAGAAAATCAGCTTACAATGGCCGAATATTTTTTAGAAAATGGTGCAAACGTAG
  AAGCAAGTGATGCTGAAGGAAGGACAGCACTTCATGTGTCTTGTTGGCAAGGCCATATGGAAATGGTGCA
  GGTCCTGATAGCATACCATGCTGACGTCAATGCTGCAGACAATGAAAAGCGCTCTGCTTTGCAGTCTGCA
  GCCTGGCAGGGCCATGTAAAAGTGGTTCAGCTTCTGATTGAGCATGGTGCTGTAGTTGACCATACATGTA
  ACCAAGGTGCAACTGCACTCTGTATTGCAGCCCAGGAAGGGCACATTGATGTTGTTCAGGTCTTATTAGA
  GCATGGTGCTGATCCAAACCATGCTGATCAATTTGGACGCACTGCTATGCGTGTTGCAGCCAAAAATGGA
  CATTCTCAGATAATTAAATTATTAGAAAAATATGGTGCATCTAGTTTGAATGGCTGTTCCCCATCTCCTG
  TTCACACAATGGAGCAAAAACCTCTACAGTCATTGTCTTCAAAAGTGCAGTCATTAACAATTAAATCAAA
  TAGCTCTGGTAGTACTGGTGGAGGGGATATGCAGCCTTCGTTACGTGGTTTACCTAATGGGCCTACTCAT
  GCTTTTAGTTCTCCTTCAGAATCTCCAGATTCTACAGTTGACCGGCAGAAGTCATCACTGTCAAATAATT
  CCCTGAAAAGCTCAAAAAATTCATCTTTGAGAACTACTTCATCTACAGCAACGGCTCAAACAGTGCCAAT
  TGATAGCTTTCATAACTTGTCATTTACAGAACAAATTCAGCAGCATTCATTGCCACGCAGTAGAAGTCGA
  CAGTCAATTGTTTCCCCATCTTCCACAACACAGTCCTTAGGACAGAGTCATAATTCACCAAGTAGTGAAT
  TTGAGTGGAGTCAAGTAAAGCCCAGTTTGAAGTCAACTAAAGCAAGTAAAGGGGGGAAATCAGAAAATTC
  TGCCAAGTCTGGATCAGCTGGGAAAAAAGCGAAACAAAGTAATTCTTCACAGCCAAAGGTTTTAGAATAT
  GAAATGACTCAGTTTGATAGAAGAGGACCTATAGCCAAATCCGGGACTGCTGCACCACCTAAACAAATGC
  CAGCAGAATCTCAATGCAAAATTATGATACCTTCAGCTCAGCAGGAAATTGGTCGATCTCAACAGCAGTT
  TCTTATTCACCAACAAAGTGGGGAACAGAAGAAGAGAAATGGAATAATGACAAATCCAAATTATCATCTT
  CAGAGCAACCAGGTTTTTCTTGGTAGGGTTTCAGTCCCACGAACAATGCAAGATAGAGGGCATCAGGAAG
  TGTTGGAGGGATACCCTTCCTCAGAGACAGAATTAAGCCTTAAACAAGCTCTGAAGCTTCAGATTGAAGG
  TTCTGACCCTAGCTTCAACTATAAAAAGGAAACACCATTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230682 representing NM_001167882
  Red=Cloning site Green=Tags(s)
  
  MKPPQQSLYLLVDSVDEGCNITEGEQTSTSLSGTVAALLAGHHEFFPPWLLLLCSARKQSKAVTKMFTGF
  RKISLDDLRKAYIVKDVQQYILHRLDQEEALRQHLTKETAEMLNQLHIKSSGCFLYLERVLDGVVENFIM
  LREIRDIPGTLNGLYLWLCQRLFVRKQFAKVQPILNVILAACRPLTITELYHAVWTKNMSLTLEDFQRKL
  DILSKLLVDGLGNTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMLAMSYTCQAKNLTPLEAQEFALHL
  INSNLQLETAELALWMIWNGTPVRDSLSTLIPKEQEVLQLLVKAGAHVNSEDDRTSCIVRQALEREDSIR
  TLLDNGASVNQCDSNGRTLLANAAYSGSLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIG
  CGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAE
  VNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGA
  EVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDS
  IDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDND
  GRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDADGRPT
  LYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQSA
  AWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNG
  HSQIIKLLEKYGASSLNGCSPSPVHTMEQKPLQSLSSKVQSLTIKSNSSGSTGGGDMQPSLRGLPNGPTH
  AFSSPSESPDSTVDRQKSSLSNNSLKSSKNSSLRTTSSTATAQTVPIDSFHNLSFTEQIQQHSLPRSRSR
  QSIVSPSSTTQSLGQSHNSPSSEFEWSQVKPSLKSTKASKGGKSENSAKSGSAGKKAKQSNSSQPKVLEY
  EMTQFDRRGPIAKSGTAAPPKQMPAESQCKIMIPSAQQEIGRSQQQFLIHQQSGEQKKRNGIMTNPNYHL
  QSNQVFLGRVSVPRTMQDRGHQEVLEGYPSSETELSLKQALKLQIEGSDPSFNYKKETPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001167882
• ORF Size: 3750 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001167882.2
• RefSeq ORF: 3753 bp
• Locus ID: 57182
• UniProt ID: Q9ULJ7
• Cytogenetics: 4q28.1
• MW: 136.9 kDa
• Gene Summary: Involved in the endosome-to-plasma membrane trafficking and recycling of SNX27-retromer-dependent cargo proteins, such as GLUT1 (PubMed:25278552).[UniProtKB/Swiss-Prot Function]