VARS2 (NM_001167733) Human Tagged ORF Clone
CAT#: RC230585
- TrueORF®
VARS2 (Myc-DDK-tagged)-Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), transcript variant 3
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001167733" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | VARS2 |
Synonyms | COXPD20; VALRS; VARS2L; VARSL |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC230585 representing NM_001167733
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTATCCCACCTCCCAATGTCACTGGCTCCCTGCACATTGGCCACGCACTCACGGTGGCCATACAGG ATGCCCTCGTGCGCTGGCACCGGATGCGTGGGGATCAAGTGCTGTGGGTCCCTGGTTCAGATCATGCAGG AATTGCTACACAAGCTGTGGTGGAGAAACAACTGTGGAAGGAACGGGGAGTGAGGAGACATGAGCTGAGC CGGGAGGCCTTCCTTAGGGAGGTGTGGCAGTGGAAGGAGGCGAAAGGTGGAGAGATCTGTGAGCAGCTGC GAGCTCTGGGTGCCTCCCTGGACTGGGATCGAGAGTGTTTTACCATGGATGTTGGCTCCTCAGTGGCTGT GACTGAAGCTTTTGTGCGGCTCTACAAGGCGGGGTTGCTGTACCGGAACCATCAGCTTGTCAACTGGTCA TGTGCTTTAAGATCAGCCATCTCGGACATTGAGGTGGAGAACCGGCCCCTGCCTGGCCACACACAGCTTC GACTGCCTGGCTGCCCCACCCCCGTGTCTTTTGGCCTCCTATTTTCTGTTGCCTTCCCCGTGGATGGAGA GCCTGATGCAGAGGTTGTGGTAGGAACCACAAGGCCAGAGACGCTGCCTGGAGATGTGGCTGTGGCCGTT CATCCAGACGACTCGCGATACACACATCTACACGGGCGACAGCTTCGTCACCCCTTGATGGGGCAGCCTC TTCCCCTCATCACAGACTATGCTGTTCAGCCACATGTGGGCACGGGGGCAGTGAAGGTGACTCCAGCTCA CAGTCCTGCCGATGCTGAGATGGGGGCCCGACATGGCTTGAGCCCCTTGAATGTCATTGCGGAGGATGGG ACCATGACCTCCCTCTGCGGGGACTGGCTGCAGGGTCTTCACCGGTTTGTGGCCCGGGAAAAGATAATGT CTGTGCTGAGTGAATGGGGCCTGTTCCGGGGCCTCCAGAACCACCCCATGGTACTGCCCATCTGCAGCCG TTCTGGGGATGTGATAGAATACCTGCTGAAGAACCAGTGGTTTGTCCGCTGCCAGGAAATGGGGGCCCGA GCTGCCAAGGCTGTGGAGTCGGGGGCCCTGGAGCTCAGTCCCTCCTTCCACCAGAAGAACTGGCAGCACT GGTTTTCCCATATTGGGGACTGGTGTGTCTCCCGGCAGCTGTGGTGGGGCCATCAGATTCCAGCCTACCT GGTTGTAGAGGACCATGCGCAGGGAGAAGAGGACTGTTGGGTGGTTGGGCGGTCAGAGGCTGAGGCCAGA GAGGTAGCAGCGGAACTGACAGGGAGGCCAGGGGCAGAGCTGACCCTGGAGAGGGATCCTGATGTCCTAG ACACATGGTTTTCTTCTGCCCTGTTCCCCTTTTCTGCCCTGGGCTGGCCCCAAGAGACCCCAGACCTTGC TCGTTTCTACCCCCTGTCACTTTTGGAAACGGGCAGCGACCTTCTGCTGTTCTGGGTGGGCCGCATGGTC ATGTTGGGGACCCAGCTCACAGGGCAGCTGCCCTTCAGCAAGGTGCTTCTTCATCCCATGGTTCGGGACA GGCAGGGCCGGAAGATGAGCAAGTCCCTGGGGAATGTGCTGGACCCAAGAGACATCATCAGTGGGGTGGA GATGCAGGTGCTGCAGGAAAAGCTGAGAAGCGGAAATTTGGACCCTGCAGAGCTGGCCATTGTGGCTGCA GCACAGAAAAAGGACTTTCCTCACGGGATCCCTGAGTGTGGGACAGATGCCCTGAGATTCACACTCTGCT CCCATGGAGTTCAGGCGGGCGACTTGCACCTGTCAGTCTCTGAGGTCCAGAGCTGCCGACATTTCTGCAA CAAGATCTGGAATGCTCTTCGCTTTATCCTCAATGCTTTAGGGGAGAAATTTGTGCCACAGCCTGCTGAG GAGCTGTCTCCCTCCTCCCCGATGGATGCCTGGATCCTGAGCCGCCTTGCCCTGGCTGCCCAGGAGTGTG AGCGGGGCTTCCTCACCCGAGAGCTCTCGCTCGTCACTCATGCCCTGCACCACTTCTGGCTTCACAACCT CTGTGACGTCTACCTGGAGGCTGTGAAGCCCGTGCTGTGGCACTCGCCCCGCCCCCTGGGGCCCCCTCAG GTCCTGTTCTCCTGCGCTGACCTCGGCCTCCGCCTCCTGGCCCCACTGATGCCCTTCCTGGCTGAAGAGC TCTGGCAGAGGCTGCCCCCCAGGCCTGGTTGCCCCCCTGCCCCCAGCATCTCGGTTGCCCCCTACCCCAG CGCCTGCAGCTTGGAGCACTGGCGCCAGCCAGAGCTGGAGCGGCGCTTCTCCCGGGTCCAAGAGGTCGTG CAGGTGCTAAGGGCTCTCCGAGCCACGTACCAGCTCACCAAAGCCCGGCCCCGAGTGCTGCTGCAGAGCT CAGAGCCTGGGGACCAGGGCCTCTTCGAGGCCTTCTTGGAGCCCCTGGGCACCCTGGGCTACTGTGGGGC TGTGGGCCTGTTACCCCCAGGCGCAGCAGCTCCCTCCGGCTGGGCCCAGGCTCCACTCAGTGACACGGCT CAAGTCTACATGGAGCTGCAGGGCCTGGTGGACCCGCAGATCCAGCTACCTCTGTTAGCCGCCCGAAGGT ACAAGTTGCAGAAGCAGCTTGACAGCCTCACAGCCAGGACCCCATCAGAAGGGGAGGCAGGGACTCAGAG GCAACAAAAGCTTTCTTCCCTCCAGCTGGAATTGTCAAAACTGGACAAGGCAGCCTCTCACCTCCGGCAG CTGATGGATGAGCCTCCAGCCCCAGGGAGCCCGGAGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230585 representing NM_001167733
Red=Cloning site Green=Tags(s) MCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDQVLWVPGSDHAGIATQAVVEKQLWKERGVRRHELS REAFLREVWQWKEAKGGEICEQLRALGASLDWDRECFTMDVGSSVAVTEAFVRLYKAGLLYRNHQLVNWS CALRSAISDIEVENRPLPGHTQLRLPGCPTPVSFGLLFSVAFPVDGEPDAEVVVGTTRPETLPGDVAVAV HPDDSRYTHLHGRQLRHPLMGQPLPLITDYAVQPHVGTGAVKVTPAHSPADAEMGARHGLSPLNVIAEDG TMTSLCGDWLQGLHRFVAREKIMSVLSEWGLFRGLQNHPMVLPICSRSGDVIEYLLKNQWFVRCQEMGAR AAKAVESGALELSPSFHQKNWQHWFSHIGDWCVSRQLWWGHQIPAYLVVEDHAQGEEDCWVVGRSEAEAR EVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPQETPDLARFYPLSLLETGSDLLLFWVGRMV MLGTQLTGQLPFSKVLLHPMVRDRQGRKMSKSLGNVLDPRDIISGVEMQVLQEKLRSGNLDPAELAIVAA AQKKDFPHGIPECGTDALRFTLCSHGVQAGDLHLSVSEVQSCRHFCNKIWNALRFILNALGEKFVPQPAE ELSPSSPMDAWILSRLALAAQECERGFLTRELSLVTHALHHFWLHNLCDVYLEAVKPVLWHSPRPLGPPQ VLFSCADLGLRLLAPLMPFLAEELWQRLPPRPGCPPAPSISVAPYPSACSLEHWRQPELERRFSRVQEVV QVLRALRATYQLTKARPRVLLQSSEPGDQGLFEAFLEPLGTLGYCGAVGLLPPGAAAPSGWAQAPLSDTA QVYMELQGLVDPQIQLPLLAARRYKLQKQLDSLTARTPSEGEAGTQRQQKLSSLQLELSKLDKAASHLRQ LMDEPPAPGSPEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001167733 |
ORF Size | 2769 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001167733.2, NP_001161205.1 |
RefSeq Size | 3394 bp |
RefSeq ORF | 2772 bp |
Locus ID | 57176 |
UniProt ID | Q5ST30 |
Cytogenetics | 6p21.33 |
Protein Families | Druggable Genome, Transcription Factors |
Protein Pathways | Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis |
MW | 102.4 kDa |
Gene Summary | This gene encodes a mitochondrial aminoacyl-tRNA synthetase, which catalyzes the attachment of valine to tRNA(Val) for mitochondrial translation. Mutations in this gene cause combined oxidative phosphorylation deficiency-20, and are also associated with early-onset mitochondrial encephalopathies. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC230585L3 | Lenti-ORF clone of VARS2 (Myc-DDK-tagged)-Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), transcript variant 3 |
USD 1,229.00 |
|
RC230585L4 | Lenti-ORF clone of VARS2 (mGFP-tagged)-Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), transcript variant 3 |
USD 1,229.00 |
|
RG230585 | VARS2 (tGFP-tagged) - Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), transcript variant 3 |
USD 1,129.00 |
|
SC329223 | VARS2 (untagged)-Human valyl-tRNA synthetase 2 mitochondrial (putative) (VARS2) transcript variant 3 |
USD 931.00 |
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