FAM59B (GAREM2) (NM_001168241) Human Tagged ORF Clone

CAT#: RC230553

GAREML (Myc-DDK-tagged)-Human family with sequence similarity 59, member B (FAM59B), transcript variant 1

Product Images

Circular map for RC230553

• 

  Western blot validation of overexpression lysate (Cat# LY433553) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC230553 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FAM59B
• Synonyms: FAM59B; GAREML
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230553 representing NM_001168241
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAGCTGGCGGCCGGGCTGGCCGGCCTGCGCTGGAGCATGGGCGCCTTCCCGCTCGACCTCATCG
  TCAGCCGCTGCCGCCTGCCCACGCTCGCCTGCCTTGGGCCAGGGGAGTACGCCGAGGGCGTCAGTGAGCG
  AGACATCCTGCTCATCCACTCCTGCCGGCAGTGGACAACGGTGACAGCTCATACCCTGGAGGAGGGCCAC
  TATGTCATCGGGCCCAAGATCGACATCCCCCTGCAGTACCCAGGGAAGTTCAAGCTCCTGGAACAGGCCC
  GGGATGTGCGGGAGCCAGTGAGGTACTTCAGCAGCGTGGAGGAGGTGGCCAGTGTCTTCCCTGACCGCAT
  CTTCGTGATGGAAGCCATCACCTTCAGCGTCAAGGTGGTGTCGGGCGAGTTCAGCGAGGACAGCGAGGTG
  TACAACTTCACGCTGCATGCGGGCGACGAGCTCACTCTTATGGGCCAGGCGGAGATCCTGTGCGCCAAGA
  CCACCAAGGAGCGCTCGCGCTTCACCACCCTCCTGCGAAAGCTGGGCCGGGCCGGGGCGCTGGCCGGGGT
  GGGCGGCGGCGGCCCAGCGAGCGCGGGGGCCGCGGGAGGCACTGGCGGCGGGGGCGCCAGGCCGGTCAAA
  GGCAAGATGCCCTGCCTCATCTGCATGAACCACCGCACCAACGAAAGCCTGAGCCTGCCCTTTCAGTGCC
  AGGGCCGCTTCAGCACTCGCAGCCCGCTGGAGCTGCAGATGCAAGAGGGCGAGCACACGGTGCGCGCCAT
  CATCGAGCGCGTGAGGCTGCCGGTGAACGTGCTGGTGCCCAGCCGGCCGCCGCGCAACCCCTACGACCTG
  CACCCGGTGCGGGAGGGTCATTGCTACAAGCTGGTTAGCATCATCTCCAAGACGGTGGTGCTGGGGCTGG
  CGCTGCGCCGCGAGGGCCCGGCGCCGCTGCACTTCCTGCTGCTCACGGACACGCCGCGCTTCGCGCTGCC
  GCAGGGCCTGCTGGCCGGGGACCCGCGCGTCGAGCGCCTGGTGCGCGACAGCGCCTCCTACTGCCGCGAG
  CGCTTCGACCCCGACGAGTACTCCACGGCCGTGCGCGAGGCGCCAGCGGAGCTCGCCGAAGACTGCGCCA
  GCCCGCGCCGCGCGCGCCTCTGCCTGCCCGCGCCGCGCGCCCCCGGGCTCGCCCGCGCCCCCGGCCCGCT
  AGCGCCGGCTCCCGCCGGCGAGGGCGACCAGGAGTACGTGAGCCCCGACTGGGCAGCCGCGCCCGAGCCC
  GCCGCGCCGCCCGCCGAGATCCCCTACGAGGAGTTGTGGGCGCACCAGGGGCCCGAGGGCCTCGTCCGGC
  CGCCCCCAGGGCTCGATCTCATCTCCTTCGGGGCCGCGGGACCGCCGCGTCGGGAGCCGGAAGCGCCGCC
  GCCTCCAGTCCCTCCCAAATCCGAGGCGGTGAAGGAGGAGTGCCGCCTGCTCAATGCCCCTCCAGTGCCT
  CCCCGGGGTGGCAATGGCAGCGGCCGGCTCTCCAGCAGCCCCCCGGTTCCCCCTCGCTTCCCCAAGCTGC
  AGCCGGTACATTCCCCCAGCTCCAGCCTCTCCTACTACTCCTCTGGCCTCCAGGATGGGGCGGGTTCCCG
  CAGTGGCAGTGGCTCCCCATCGCCGGACACCTACTCCCTCTATTGCTACCCATGCACCTGGGGAGACTGC
  AAGGTGGGCGAGTCCTCTAGCCGCCCAGCCCCCGGTCCCCTACCCTCAACCACACAGCCCAGCCAGGCCT
  CCCGGGCCCTCACAGAGCCTCTGAGCGGTCGAGCCGCCTCCCTTCTGGGGGCTGACACCCCTGTTAAGAC
  CTACCACAGCTGCCCTCCTCTATTCAAGCCCTCACATCCCCAGAAGCGCTTTGCTCCGTTTGGAGCTCTC
  AACCCTTTTTCCGGGCCTGCCTACCCCTCAGGCCCTTCAGCGGCCTTGTCTTCTGGGCCCAGAACCACCT
  CGGGTCCTGTGGCTACCTCTGGCCCTGCGTATTCCCCAGGCCCAGCCTCGCCAGGCCAGGCCTATTCAGC
  TGCTCCCCCCTCCTCCTGCGCCCCCTCCTCCTCCTCTTCTTCTGAATGGCAGGAACCAGTCCTGGAGCCC
  TTCGATCCCTTTGAGCTGGGGCAGGGCAGTTCTCCAGAGCCTGAGCTGCTGCGTTCTCAGGAGCCCAGAG
  CAGTGGGGACACCTGGGCCTGGACCCCGCCTTTCACCACTTGGCCCCTCCAAGGCCTTTGAGCCTGAAGG
  TTTGGTGCTGCACCAGGTCCCCACCCCACTGTCACCAGCTGCTCTGCAGGGACCCGAGGCGGGAGGAGCA
  CTTTTTCTAACCCAAGGGCGCCTGGAAGGGCCTCCTGCCAGTCCCCGGGATGGAGCCACAGGCTTTGGAG
  TCCGAGATGCCTCCTCCTGGCAGCCCCCTGCTGACCTGTCTGCACTCTCCCTGGAGGAGGTCTCTCGCAG
  TCTGCGTTTCATCGGGCTCTCAGAGGATGTGGTGAGCTTCTTTGCCCGAGAACGCATCGATGGTAGCATC
  TTTGTGCAGCTCAGTGAGGACATCCTGGCAGATGACTTCCACCTCACCAAGCTGCAGGTCAAGAAGATCA
  TGCAGTTCATCAAAGGCTGGAGGCCCAAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230553 representing NM_001168241
  Red=Cloning site Green=Tags(s)
  
  MEKLAAGLAGLRWSMGAFPLDLIVSRCRLPTLACLGPGEYAEGVSERDILLIHSCRQWTTVTAHTLEEGH
  YVIGPKIDIPLQYPGKFKLLEQARDVREPVRYFSSVEEVASVFPDRIFVMEAITFSVKVVSGEFSEDSEV
  YNFTLHAGDELTLMGQAEILCAKTTKERSRFTTLLRKLGRAGALAGVGGGGPASAGAAGGTGGGGARPVK
  GKMPCLICMNHRTNESLSLPFQCQGRFSTRSPLELQMQEGEHTVRAIIERVRLPVNVLVPSRPPRNPYDL
  HPVREGHCYKLVSIISKTVVLGLALRREGPAPLHFLLLTDTPRFALPQGLLAGDPRVERLVRDSASYCRE
  RFDPDEYSTAVREAPAELAEDCASPRRARLCLPAPRAPGLARAPGPLAPAPAGEGDQEYVSPDWAAAPEP
  AAPPAEIPYEELWAHQGPEGLVRPPPGLDLISFGAAGPPRREPEAPPPPVPPKSEAVKEECRLLNAPPVP
  PRGGNGSGRLSSSPPVPPRFPKLQPVHSPSSSLSYYSSGLQDGAGSRSGSGSPSPDTYSLYCYPCTWGDC
  KVGESSSRPAPGPLPSTTQPSQASRALTEPLSGRAASLLGADTPVKTYHSCPPLFKPSHPQKRFAPFGAL
  NPFSGPAYPSGPSAALSSGPRTTSGPVATSGPAYSPGPASPGQAYSAAPPSSCAPSSSSSSEWQEPVLEP
  FDPFELGQGSSPEPELLRSQEPRAVGTPGPGPRLSPLGPSKAFEPEGLVLHQVPTPLSPAALQGPEAGGA
  LFLTQGRLEGPPASPRDGATGFGVRDASSWQPPADLSALSLEEVSRSLRFIGLSEDVVSFFARERIDGSI
  FVQLSEDILADDFHLTKLQVKKIMQFIKGWRPKI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001168241
• ORF Size: 2622 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001168241.2
• RefSeq ORF: 2625 bp
• Locus ID: 150946
• UniProt ID: Q75VX8
• Cytogenetics: 2p23.3
• MW: 93.3 kDa
• Gene Summary: Probable adapter protein that may provide a link between cell surface epidermal growth factor receptor and the MAPK/ERK signaling pathway.[UniProtKB/Swiss-Prot Function]