Semaphorin 3E (SEMA3E) (NM_001178129) Human Tagged ORF Clone

CAT#: RC230452

SEMA3E (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (SEMA3E), transcript variant 2

Product Images

Circular map for RC230452

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Semaphorin 3E
• Synonyms: coll-5; M-SEMAH; M-SemaK; SEMAH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230452 representing NM_001178129
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTGGATGAATATCAAGAGAGGCTCTTCGTGGGAGGCAGGGACCTTGTATATTCCCTCAGCTTGG
  AGAGAATCAGTGACGGCTATAAAGAGATACACTGGCCGAGTACAGCTCTAAAAATGGAAGAATGCATAAT
  GAAGGGAAAAGATGCGGGTGAATGTGCAAATTATGTTCGGGTTTTGCATCACTATAACAGGACACACCTT
  CTGACCTGTGGTACTGGAGCTTTTGATCCAGTTTGTGCCTTCATCAGAGTTGGATATCATTTGGAGGATC
  CTCTGTTTCACCTGGAATCACCCAGATCTGAGAGAGGAAGGGGCAGATGTCCTTTTGACCCCAGCTCCTC
  CTTCATCTCCACTTTAATTGGTAGTGAATTGTTTGCTGGACTCTACAGTGACTACTGGAGCAGAGACGCT
  GCGATCTTCCGCAGCATGGGGCGACTGGCCCATATCCGCACTGAGCATGACGATGAGCGTCTGTTGAAAG
  AACCAAAATTTGTAGGTTCATACATGATTCCTGACAATGAAGACAGAGATGACAACAAAGTATATTTCTT
  TTTTACTGAGAAGGCACTGGAGGCAGAAAACAATGCTCACGCAATTTACACCAGGGTCGGGCGACTCTGT
  GTGAATGATGTAGGAGGGCAGAGAATACTGGTGAATAAGTGGAGCACTTTCCTAAAAGCGAGACTCGTTT
  GCTCAGTACCAGGAATGAATGGAATTGACACATATTTTGATGAATTAGAGGACGTTTTTTTGCTACCTAC
  CAGAGATCATAAGAATCCAGTGATATTTGGACTCTTTAACACTACCAGTAATATTTTTCGAGGGCATGCT
  ATATGTGTCTATCACATGTCTAGCATTCGGGCAGCCTTCAACGGACCATATGCACATAAGGAAGGACCTG
  AATACCACTGGTCAGTCTATGAAGGAAAAGTCCCTTATCCAAGGCCTGGTTCTTGTGCCAGCAAAGTAAA
  TGGAGGGAGATACGGAACCACCAAGGACTATCCTGATGATGCCATCCGATTTGCAAGAAGTCATCCACTA
  ATGTACCAGGCCATAAAACCTGCCCATAAAAAACCAATATTGGTAAAAACAGATGGAAAATATAACCTGA
  AACAAATAGCAGTAGATCGAGTGGAAGCTGAGGATGGCCAATATGACGTCTTGTTTATTGGGACAGATAA
  TGGAATTGTGCTGAAAGTAATCACAATTTACAACCAAGAAATGGAATCAATGGAAGAAGTAATTCTAGAA
  GAACTTCAGATATTCAAGGATCCAGTTCCTATTATTTCTATGGAGATTTCTTCAAAGCGGCAACAGCTGT
  ATATTGGATCTGCTTCTGCTGTGGCTCAAGTCAGATTCCATCACTGTGACATGTATGGAAGTGCTTGTGC
  TGACTGCTGCCTGGCTCGAGACCCTTACTGTGCCTGGGATGGCATATCCTGCTCCCGGTATTACCCAACA
  GGCACACATGCAAAAAGGCGTTTCCGGAGACAAGATGTTCGACATGGAAATGCAGCTCAGCAGTGCTTTG
  GACAACAGTTTGTTGGGGATGCTTTGGATAAGACTGAAGAACATCTGGCTTATGGCATAGAGAACAACAG
  TACTTTGCTGGAATGTACCCCACGATCTTTACAAGCGAAAGTTATCTGGTTTGTACAGAAAGGACGTGAG
  ACAAGAAAAGAGGAGGTGAAGACAGATGACAGAGTGGTTAAGATGGACCTTGGTTTACTCTTCCTAAGGT
  TACACAAATCAGATGCTGGGACCTATTTTTGCCAGACAGTAGAGCATAGCTTTGTCCATACGGTCCGTAA
  AATCACCTTGGAGGTAGTGGAAGAGGAGAAAGTCGAGGATATGTTTAACAAGGACGATGAGGAGGACAGG
  CATCACAGGATGCCTTGTCCTGCTCAGAGTAGCATCTCGCAGGGAGCAAAACCATGGTACAAGGAATTCT
  TGCAGCTGATCGGTTATAGCAACTTCCAGAGAGTGGAAGAATACTGCGAGAAAGTATGGTGCACAGATAG
  AAAGAGGAAAAAGCTTAAAATGTCACCCTCCAAGTGGAAGTATGCCAACCCTCAGGAAAAGAAGCTCCGT
  TCCAAACCTGAGCATTACCGCCTGCCCAGGCACACGCTGGACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230452 representing NM_001178129
  Red=Cloning site Green=Tags(s)
  
  MLLDEYQERLFVGGRDLVYSLSLERISDGYKEIHWPSTALKMEECIMKGKDAGECANYVRVLHHYNRTHL
  LTCGTGAFDPVCAFIRVGYHLEDPLFHLESPRSERGRGRCPFDPSSSFISTLIGSELFAGLYSDYWSRDA
  AIFRSMGRLAHIRTEHDDERLLKEPKFVGSYMIPDNEDRDDNKVYFFFTEKALEAENNAHAIYTRVGRLC
  VNDVGGQRILVNKWSTFLKARLVCSVPGMNGIDTYFDELEDVFLLPTRDHKNPVIFGLFNTTSNIFRGHA
  ICVYHMSSIRAAFNGPYAHKEGPEYHWSVYEGKVPYPRPGSCASKVNGGRYGTTKDYPDDAIRFARSHPL
  MYQAIKPAHKKPILVKTDGKYNLKQIAVDRVEAEDGQYDVLFIGTDNGIVLKVITIYNQEMESMEEVILE
  ELQIFKDPVPIISMEISSKRQQLYIGSASAVAQVRFHHCDMYGSACADCCLARDPYCAWDGISCSRYYPT
  GTHAKRRFRRQDVRHGNAAQQCFGQQFVGDALDKTEEHLAYGIENNSTLLECTPRSLQAKVIWFVQKGRE
  TRKEEVKTDDRVVKMDLGLLFLRLHKSDAGTYFCQTVEHSFVHTVRKITLEVVEEEKVEDMFNKDDEEDR
  HHRMPCPAQSSISQGAKPWYKEFLQLIGYSNFQRVEEYCEKVWCTDRKRKKLKMSPSKWKYANPQEKKLR
  SKPEHYRLPRHTLDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001178129
• ORF Size: 2145 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001178129.1, NP_001171600.1
• RefSeq ORF: 2148 bp
• Locus ID: 9723
• UniProt ID: O15041
• Cytogenetics: 7q21.11
• Protein Families: Secreted Protein
• Protein Pathways: Axon guidance
• MW: 82.9 kDa
• Gene Summary: Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene encodes a class 3 semaphorin. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]